AptaDB: Result

Interaction ID: 170

Aptamer sequence: TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA

Target unique ID: P04275

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_165	VWF R9.14	Protein	VWF_HUMAN	12 nM	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)	17854267

Aptamer Sequence:	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA
The optimal secondary structure in dot-bracket notation:	.........(((..(((((.((......)).))))).)))
The centroid secondary structure in dot-bracket notation:	.........(((..(((((.((......)).))))).)))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_165	Description	VWF R9.14
Aptamer chemistry	DNA	Length	40 nt
GC content	52.5%	Molecular weight	12,462.79 Da
Molarity of 1 μg/μl solution	80.24 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	TGGACGAACTGCCCTCAGCTACTTTCATGTTGCTGACGCA
Applications	Inhibit platelet aggregation in PFA-100 and ristocetin-induced platelet aggregation assays

Type	Detail	Type	Detail
Uniprot ID	P04275	Protein name	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Gene name(s)	VWF F8VWF	Organism	von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]
Status	reviewed in uniprot database	Length	2813
Mass	309,265	Activity regulation	N/A
Involvement in disease	von Willebrand disease 1 (VWD1)	Catalytic activity	N/A
PDB ID(s)	1AO3;1ATZ;1AUQ;1FE8;1FNS;1IJB;1IJK;1M10;1OAK;1SQ0;1U0N;1UEX;2ADF;2MHP;2MHQ;3GXB;3HXO;3HXQ;3PPV;3PPW;3PPX;3PPY;3ZQK;4C29;4C2A;4C2B;4DMU;4NT5;5BV8;6FWN;6N29;7EOW;7F49;7KWO;7PMV;7PNF;
Function	Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V.

	Interaction ID	170
	Target type	Protein
	Target unique ID	P04275
	Activity	12 nM
	Binding Conditions/Buffer	Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA); Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
	Assay	Binding affinities of VWF aptamers
	PubMed ID	17854267

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1215	DNA	GTCGAATCAGCACCTCTGCATAGGTTACGTTTATACTGCG	57.50%
Apta_308	DNA	TGGCGGTACGGGCCGTGCACCCACTTACCTGGGAAGTGA	55.00%
Apta_573	DNA	CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA	55.00%
Apta_853	DNA	TGACCGAGTGTCTGGTCATTTTCGATGTCTGTTGTTAGGC	55.00%
Apta_1287	DNA	GCCCGCATGCCGTCAAGCAATGTTAATCTTGTTTTGATTTCG	54.76%
Apta_1285	DNA	AGGAACCTTCTCTTCCTCTATTTTTTAATTCTGTCTACCCT	53.66%
Apta_1172	DNA	GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA	52.63%
Apta_34	DNA	TGTCACAATTTCCCCCCTCGCACATTTTGCAAGTAACTGA	52.50%
Apta_36	DNA	GTTAATGGGGGATCTCGCGGCCGTTCTTGTTGCTTATACA	52.50%
Apta_60	DNA	GGAATGAGTGCCCTGCAAGCGAGGGCTAGC	52.50%
Apta_361	DNA	GGGAACGCCCGTATTGCTCGGTTCCC	52.50%
Apta_362	DNA	GGGAACGCACCGATCGCAGGTTTCCC	52.50%
Apta_423	DNA	TGGGAGCTGATGTCGCATGGGTTTTGATCACATGA	52.50%
Apta_462	DNA	GGTATAGACCCCTGAGTCCTACCGAGGGCACGGCA	52.50%
Apta_602	DNA	GGGCGAGAACAGTGTCCCAGTCGCTAGTTTTCTCTGCCC	52.50%
Apta_877	DNA	GTGCGAAGCGCCTCCACTGTACATCCACTCCTTCTGCC	52.50%
Apta_917	DNA	TCGATTTCCTTAGTTGTCTTCCTTAGTGAG	52.50%
Apta_963	DNA	TGGCGCTGCCCCTAGTCTCCGGCTGATAGCTGCTTCTTGG	52.50%
Apta_1243	DNA	GGTGTTGTTGTTGGCTAGTTTGTTGTGGGC	52.50%
Apta_1244	DNA	GGAGATGTCAGTTGTGTGTGTGGTTATGGC	52.50%

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