Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_251 | 2mHNE-3 | Protein | ELNE_HUMAN | 120 nM | 20 mM HEPES, pH 7.5, 150 mM NaCL, 6 mM KCL, 2 mM MgCl2 | 29568464 |
Structure information of aptamer
| Aptamer Sequence: | GCCCTACATTCTGTGCGACTCCCCCCCCCGC |
| The optimal secondary structure in dot-bracket notation: | ..............(((...........))) |
| The centroid secondary structure in dot-bracket notation: | ..............(((...........))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_251 | Description | 2mHNE-3 |
| Aptamer chemistry | DNA | Length | 31 nt |
| GC content | 71.0% | Molecular weight | 9,504.88 Da |
| Molarity of 1 μg/μl solution | 105.21 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | GCCCTACATTCTGTGCGACTCCCCCCCCCGC | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P08246 | Protein name | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Gene name(s) | ELANE ELA2 | Organism | Neutrophil elastase (EC 3.4.21.37) (Bone marrow serine protease) (Elastase-2) (Human leukocyte elastase) (HLE) (Medullasin) (PMN elastase) |
| Status | reviewed in uniprot database | Length | 267 |
| Mass | 28,518 | Activity regulation | N/A |
| Involvement in disease | Cyclic haematopoiesis (CH) | Catalytic activity | Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa |
| PDB ID(s) | 1B0F;1H1B;1HNE;1PPF;1PPG;2RG3;2Z7F;3Q76;3Q77;4NZL;4WVP;5A09;5A0A;5A0B;5A0C;5A8X;5A8Y;5A8Z;5ABW;6E69;6F5M;6SMA;7CBK; |
 |
Function | Modifies the functions of natural killer cells, monocytes and granulocytes. |
Activity data
|
No relevant experimental diagram |
Interaction ID | 256 |
| Target type | Protein | |
| Target unique ID | P08246 | |
| Activity | 120 nM | |
| Binding Conditions/Buffer | 20 mM HEPES, pH 7.5, 150 mM NaCL, 6 mM KCL, 2 mM MgCl2 |
|
| Assay | N/A |
|
| PubMed ID | 29568464 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_252 | DNA | CCCTTACCTACTCTGCTCCCCCCCCCCCTCGGCTGC | |
| Apta_1084 | DNA | TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC | |
| Apta_1269 | DNA | TAGCACACAACCCTTTGATTGGGATATCTCATCCCTCG | |
| Apta_871 | DNA | CCAACCACACATCCTTCCATCGACATGGACCCACCGTTCC | |
| Apta_1214 | DNA | GTCCATTATTCTGGTAGCGTTGAACAACATTCAACACGCC | |
| Apta_253 | DNA | CCCTGTTCGCTATCCCCCATCCCCCGATTGC | |
| Apta_434 | DNA | ACACGACGGGAATACTGACTCTCCCCCATGT | |
| Apta_1086 | DNA | GCAGTTGATCCTTTGGATACCCTGG | |
| Apta_1146 | DNA | GGGACAGGGAGTGCGCTGCTCCCC | |
| Apta_1027 | DNA | GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC | |
| Apta_42 | DNA | GCTGGTCTCTGCGGGTTGTTGCGCCGCGGCACCCTTGGCA | |
| Apta_222 | DNA | AGCGACTGACGATCTTGAGTAAACCGCTCATCCACGTAGT | |
| Apta_386 | DNA | GGAAAAGATGGTATGGGCCCTAGAAAGTCCACCCGGCGCC | |
| Apta_472 | DNA | CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA | |
| Apta_473 | DNA | CCCTGCACTCGCAATGGCCCCTCTACACCACAAACGGACA | |
| Apta_476 | DNA | CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG | |
| Apta_857 | DNA | GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC | |
| Apta_875 | DNA | GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG | |
| Apta_937 | DNA | TTGCCCACAGATCTGTGGAAGCCGAACCGCGTGCTAGTCGTG | |
| Apta_939 | DNA | TTGCCCACCGTACTGTGCAGGTCGAACTACAGGCACGTCGTG |
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