AptaDB: Result

Interaction ID: 232

Aptamer sequence: ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG

Target unique ID: O14786

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1036	SL12	Protein	NRP1_HUMAN	5 nM	PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer	22359573

Aptamer Sequence:	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG
The optimal secondary structure in dot-bracket notation:	...(((((.......))))).((((.((....))))))
The centroid secondary structure in dot-bracket notation:	...(((((.......))))).((((.((....))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1036	Description	SL12
Aptamer chemistry	DNA	Length	38 nt
GC content	52.6%	Molecular weight	11,700.51 Da
Molarity of 1 μg/μl solution	85.47 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG
Applications	Identifying high affinity aptamer sequences for the biological molecules and targeting them as antagonist for cancer cell detection

Type	Detail	Type	Detail
Uniprot ID	O14786	Protein name	Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)
Gene name(s)	NRP1 NRP VEGF165R	Organism	Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)
Status	reviewed in uniprot database	Length	923
Mass	103,134	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1KEX;2QQI;2QQM;2QQN;3I97;4DEQ;4RN5;5C7G;5IJR;5IYY;5J1X;5JGI;5JGQ;5JHK;5L73;6FMC;6FMF;6TKK;7JJC;7O1N;
Function	Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system.

No relevant experimental diagram	Interaction ID	232
	Target type	Protein
	Target unique ID	O14786
	Activity	5 nM
	Binding Conditions/Buffer	PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer
	Assay	N/A
	PubMed ID	22359573

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1040	DNA	CAATTGGGCCCGTCCGTATGGTGGGT	63.16%
Apta_744	DNA	GTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTG	57.78%
Apta_31	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGGTGTGCTGGCCAGATAGTATGTGCAATCA	57.58%
Apta_476	DNA	CCTATCTTATTCGTTGCGGACTCCCCGGTCGGTATGTATG	55.00%
Apta_853	DNA	TGACCGAGTGTCTGGTCATTTTCGATGTCTGTTGTTAGGC	55.00%
Apta_1162	DNA	AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT	53.06%
Apta_677	DNA	CCAAATGCGACGGGGCCTGTTTTAATGGGG	52.63%
Apta_917	DNA	TCGATTTCCTTAGTTGTCTTCCTTAGTGAG	52.63%
Apta_1037	DNA	ATACCAGTCTATTCAATTGG	52.63%
Apta_1206	DNA	GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG	52.63%
Apta_283	DNA	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA	52.50%
Apta_850	DNA	CACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCA	52.50%
Apta_340	DNA	TACTCAGGGCACTGCAAGCAATTGTGGTCCCAATGGGCTGAGTA	52.27%
Apta_1023	DNA	CTCCTTCAACGAAGTGGGTCTCCTTCAACGAAGTGGGTCTC	51.22%
Apta_968	DNA	TAACACGACAGACGTTCGGAGGTCGAACCCTGACAGCGTGGGC	51.16%
Apta_640	DNA	CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG	51.11%
Apta_833	DNA	ATTTCGCCCCCGTGTTCCGACTGGTATCTTCACGTCTTCGAGTGT	51.11%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	51.06%
Apta_884	DNA	ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT	50.82%
Apta_76	DNA	CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG	50.00%

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