Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 822
Aptamer sequence: ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC
Target unique ID: CRM-CCL-185
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_740 | TV04 | Cell | vaccinia virus–infected and–uninfected lung cancer A549 cells | 6.5 nM | Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA | 19246617 |
Structure information of aptamer
| Aptamer Sequence: | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC |
| The optimal secondary structure in dot-bracket notation: | .......((..((.((.....(((((((((.((((......)))).)).))))...)))....)).)).)) |
| The centroid secondary structure in dot-bracket notation: | .......((..((.((.....(((((((((.((((......)))).)).))))...)))....)).)).)) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_740 | Description | TV04 |
| Aptamer chemistry | DNA | Length | 71 nt |
| GC content | 45.1% | Molecular weight | 21,839.99 Da |
| Molarity of 1 μg/μl solution | 45.79 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | ATCGTCTGCTCCGTCCAATAAACCTGCATAATTTATAAGTCTAGACTGCTGCATTTGGTGTGAGGTCGTGC | ||
| Applications | Detection of A549 cells | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRM-CCL-185 | Cell name | vaccinia virus-infected and-uninfected lung cancer A549 cells |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | Certified reference material | Cell type | epithelial cell |
| Morphology | epithelial | Tissue | Lung |
| Disease | Carcinoma | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | N/A |
|
|
| Application(s) | Assay development | ||
Activity data
 |
Interaction ID | 822 |
| Target type | Cell | |
| Target unique ID | CRM-CCL-185 | |
| Activity | 6.5 nM | |
| Binding Conditions/Buffer | Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA |
|
| Assay | Flow cytometry assay for the binding capacity of the Cy5-labeled sequences TV04 |
|
| PubMed ID | 19246617 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_736 | DNA | ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_741 | DNA | ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_738 | DNA | ATCGTCTGCTCCGTCCAATACCTGCATATACACTTTGCATGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_739 | DNA | ATCGTCTGCTCCGTCCAATAGCGTGCATTGGTTTACTGCATCCGTGAAACTGGGCTTTGGTGTGAGGTCGTGC | |
| Apta_737 | DNA | ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC | |
| Apta_743 | DNA | ATCGTCTGCTCCGTCCAATATCCGAGCAAGAACTCATATTGCATTATTTATAGCTACGCGCTGCATTTGGTGTGAGGTCGTGC | |
| Apta_791 | DNA | ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC | |
| Apta_742 | DNA | ATCGTCTGCTCCGTCCAATAGGACCGATAGGAACCACGGACTGCATGTTTCTGCATTTGACGTGGTTTGGTGTGAGGTCGTGC | |
| Apta_532 | DNA | ATCCGTCACACCTGCTCTAACTTACACATAATTCTAGGTTACATCTTGCTCATATGGTGTTGGCTCCCGTAT | |
| Apta_972 | DNA | TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC | |
| Apta_525 | DNA | ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT | |
| Apta_906 | DNA | ATCCGTCACACCTGCTCTCCCCGGCGTCCGTTTATTAGCAGACTTTGGCGGAATTGGTGTTGGCTCCCGTAT | |
| Apta_1231 | DNA | ACCACCGGCCCGAGATAGTCTAGACCACTATGTTGTTGTGCTTACTGCTGTGTGGTGTGG | |
| Apta_657 | DNA | CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_529 | DNA | ATCCGTCACACCTGCTCTACACTCCGCACGATCTTTTTTAGAACGCGTACCCGTTGGTGTTGGCTCCCGTAT | |
| Apta_800 | DNA | AAGGAGCAGCGTGGAGGATATACACCGTCATCAGAGGGAGCATCTCTAGTCAGGACTGTGATGAATTAGGGTGTGTCGTCGTGGT | |
| Apta_592 | DNA | CTCTCGGGACGACCACTACGCATAGTTTCTATCGCCAGGAAGGGTCGTCCC | |
| Apta_970 | DNA | TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC | |
| Apta_530 | DNA | ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT | |
| Apta_944 | DNA | ATACGACTCACTATTAGGGACAAAGCTGACAACCCTTTCATAATCTAACTACATTTATGTGCTAGACTACTGACTAC |
DEV TOOL
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