AptaDB: Result

Interaction ID: 1131

Aptamer sequence: CCCUCCUACAUAGGG

Target unique ID: O43490

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1077	CD133-A15	Protein	PROM1_HUMAN	83.2 nM	Dulbecco’s phosphate buffered saline containing 2.5 mM MgCl2	23196060

Aptamer Sequence:	CCCUCCUACAUAGGG
The optimal secondary structure in dot-bracket notation:	((((.......))))
The centroid secondary structure in dot-bracket notation:	((((.......))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1077	Description	CD133-A15
Aptamer chemistry	RNA	Length	15 nt
GC content	60.0%	Molecular weight	4,710.86 Da
Molarity of 1 μg/μl solution	212.28 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	CCCUCCUACAUAGGG
Applications	Aid future development of cancer stem cell targeted therapeutics and molecular imaging

Type	Detail	Type	Detail
Uniprot ID	O43490	Protein name	Prominin-1 (Antigen AC133) (Prominin-like protein 1) (CD antigen CD133)
Gene name(s)	PROM1 PROML1 MSTP061	Organism	Prominin-1 (Antigen AC133) (Prominin-like protein 1) (CD antigen CD133)
Status	reviewed in uniprot database	Length	865
Mass	97,202	Activity regulation	N/A
Involvement in disease	Retinitis pigmentosa 41 (RP41)	Catalytic activity	N/A
PDB ID(s)	N/A
Function	May play a role in cell differentiation, proliferation and apoptosis.

	Interaction ID	1131
	Target type	Protein
	Target unique ID	O43490
	Activity	83.2 nM
	Binding Conditions/Buffer	Dulbecco’s phosphate buffered saline containing 2.5 mM MgCl2
	Assay	Determination of equilibrium dissociation constants (KD) for the interaction of truncated clones of CD133 aptamers with CD133 positive or negative cell lines
	PubMed ID	23196060

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1038	DNA	CCCGTCCGTATGGTGGG	52.94%
Apta_1148	DNA	CCGTCTTCCAGACAAGAGTGCAGGG	48.00%
Apta_1039	DNA	GCCCGTCCGTATGGTGGGT	47.37%
Apta_318	DNA	CCCGTCTTCCAGACAAGAGTGCAGGG	46.15%
Apta_1282	DNA	TCCTCTCACACATCTTATCCCCTAGGG	44.44%
Apta_925	RNA	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG	43.33%
Apta_342	RNA	GCGACUGGUUACCCGGUCG	42.11%
Apta_1078	RNA	CAGAACGUAUACUAUUCUG	42.11%
Apta_313	RNA	UGCCCUGCCCUCACCCGUUAGCCUGAGCGCCCCGCA	41.67%
Apta_319	RNA	ACCCUGAUGGUAGACGCCGGGGUG	41.67%
Apta_634	RNA	GGUCUUACGUCGUUCGCGACUAUUGGGAGACC	40.62%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	40.00%
Apta_324	DNA	CAAACAATCAAACAGCAGTGGGGTG	40.00%
Apta_324	DNA	CAAACAATCAAACAGCAGTGGGGTG	40.00%
Apta_588	RNA	CCGGUGCGCAUAACCACCUCAGUGCGAGCAAGGAA	40.00%
Apta_855	RNA	UCACUGUUAUCCGAUAGCAGCGCGGGAUGA	40.00%
Apta_1037	DNA	ATACCAGTCTATTCAATTGG	40.00%
Apta_1058	RNA	GUAAGGCAGCUUACAAACAUUAAGGCACAG	40.00%
Apta_152	RNA	UUACCACGCGCUCUUAACUGCUAGCGCCAUGGC	39.39%
Apta_373	RNA	CCAUACGGGAGAGAAACU	38.89%

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