Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1128
Aptamer sequence: CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT
Target unique ID: P01112
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1074 aptamer2 for h-Ras Protein RASH_HUMAN 900 nM 50 mM Tris-HCl 16448086
Structure information of aptamer
Aptamer Sequence: CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT
The optimal secondary structure in dot-bracket notation: ..((((((((.((((((.......((((((.((...)).))))))...........)))))).)).....))))))....
The centroid secondary structure in dot-bracket notation: ...(((((..............................................................))))).....
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1074 Description aptamer2 for h-Ras
Aptamer chemistry DNA Length 80 nt
GC content 50.0% Molecular weight 24,524.71 Da
Molarity of 1 μg/μl solution 40.78 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT
Applications Applications in cancer research, development of cancer diagnostics, and treatment of cancer
Target information
Type Detail Type Detail
Uniprot ID P01112 Protein name GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed]
Gene name(s) HRAS HRAS1 Organism GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed]
Status reviewed in uniprot database Length 189
Mass 21,298 Activity regulation Alternates between an inactive form bound to GDP and an active form bound to GTP
Involvement in disease Costello syndrome (CSTLO) Catalytic activity Reaction=GTP + H2O = GDP + H(+) + phosphate
PDB ID(s)
121P;1AA9;1AGP;1BKD;1CLU;1CRP;1CRQ;1CRR;1CTQ;1GNP;1GNQ;1GNR;1HE8;1IAQ;1IOZ;1JAH;1JAI;1K8R;1LF0;1LF5;1LFD;1NVU;1NVV;1NVW;1NVX;1P2S;1P2T;1P2U;1P2V;1PLJ;1PLK;1PLL;1Q21;1QRA;1RVD;1WQ1;1XCM;1XD2;1XJ0;1ZVQ;1ZW6;221P;2C5L;2CE2;2CL0;2CL6;2CL7;2CLC;2CLD;2EVW;2LCF;2LWI;2N42;2N46;2Q21;2QUZ;2RGA;2RGB;2RGC;2RGD;2RGE;2RGG;2UZI;2VH5;2X1V;3DDC;3I3S;3K8Y;3K9L;3K9N;3KKM;3KKN;3KUD;3L8Y;3L8Z;3LBH;3LBI;3LBN;3LO5;3OIU;3OIV;3OIW;3RRY;3RRZ;3RS0;3RS2;3RS3;3RS4;3RS5;3RS7;3RSL;3RSO;3TGP;421P;4DLR;4DLS;4DLT;4DLU;4DLV;4DLW;4DLX;4DLY;4DLZ;4DST;4DSU;4EFL;4EFM;4EFN;4G0N;4G3X;4K81;4L9S;4L9W;4NYI;4NYJ;4NYM;4Q21;4RSG;4URU;4URV;4URW;4URX;4URY;4URZ;4US0;4US1;4US2;4XVQ;4XVR;521P;5B2Z;5B30;5E95;5P21;5VBE;5VBZ;5WDO;5WDP;5WDQ;5WFO;5WFP;5WFQ;5WFR;5WPL;5X9S;5ZC6;621P;6AMB;6AXG;6BVI;6BVJ;6BVK;6BVL;6BVM;6CUO;6CUP;6CUR;6D55;6D56;6D59;6D5E;6D5G;6D5H;6D5J;6D5L;6D5M;6D5V;6D5W;6DZH;6E6C;6E6P;6MQT;6NTC;6NTD;6Q21;6V94;6V9F;6V9J;6V9L;6V9M;6V9N;6V9O;6ZJ0;6ZL3;721P;7DPH;7DPJ;7JHP;7JIF;7JIG;7JIH;7JII;7L0F;7L0G;7OG9;7OGA;7OGB;7OGC;7OGD;7OGE;7OGF;821P;
Function Involved in the activation of Ras protein signal transduction.
Activity data

No relevant experimental diagram

 Interaction ID 1128
Target type Protein
Target unique ID P01112
Activity 900 nM
Binding Conditions/Buffer

50 mM Tris-HCl
Assay

N/A
PubMed ID 16448086
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1028 DNA CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT 73.75%
Apta_1030 DNA CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT 72.50%
Apta_1075 DNA CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT 71.25%
Apta_1073 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT 70.00%
Apta_1029 DNA CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT 68.75%
Apta_1076 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT 68.75%
Apta_907 DNA ATACGGGAGCCAACACCACAACAAGATAAAGTATCAATCTGGTTGACGGACGTGAGAGCAGGTGTGACGGAT 55.00%
Apta_897 DNA ATACGGGAGCCAACACCACTCTTATTTCCGGAAGTGGTTCTTCAACAGAGGAATAGAGCAGGTGTGACGGAT 53.75%
Apta_902 DNA ATACGGGAGCCAACACCACCAACATTATCAGAGTATGTTACTTATAGTGGTGGCAGAGCAGGTGTGACGGAT 53.75%
Apta_525 DNA ATCCGTCACACCTGCTCTCAATCTGAAATATTGCAAGCAGTGCTCACAATTTGTTGGTGTTGGCTCCCGTAT 52.50%
Apta_531 DNA ATACGGGAGCCAACACCACATCGACTTACGAATCAACGCGTTTATTATTGGTTCAGAGCAGGTGTGACGGAT 52.50%
Apta_884 DNA ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT 52.50%
Apta_901 DNA ATACGGGAGCCAACACCACAAGTTGACTTGGAGGAGGGTCTAACTAGTTCGAGGAGAGCAGGTGTGACGGAT 52.50%
Apta_316 DNA CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA 51.25%
Apta_524 DNA ATCCGTCACACCTGCTCTGGCATTAGTATTCCATAGCCGGCCAAGTCTATGTAGTGGTGTTGGCTCCCGTAT 51.25%
Apta_533 DNA ATACGGGAGCCAACACCAAAGTAAGATCATCACCCGGACGCGGACATAATAGGAGAGCAGGTGTGACGGAT 51.25%
Apta_537 DNA ATCCGTCACACCTGCTCTCCTCGAACTAGTTAGACCCTCCTCCAAGTCAACTTGTGGTGTTGGCTCCCGTAT 51.25%
Apta_826 DNA ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGATGGTGTTGGCTCCCGTAT 51.25%
Apta_899 DNA ATCCGTCACACCTGCTCTCGAATAGAGTATTCGATACTGACTTTATTTACCGTCTGGTGTTGGCTCCCGTAT 51.25%
Apta_533 DNA ATACGGGAGCCAACACCAAAGTAAGATCATCACCCGGACGCGGACATAATAGGAGAGCAGGTGTGACGGAT 51.25%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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