AptaDB: Result

Interaction ID: 917

Aptamer sequence: CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG

Target unique ID: 25922

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_831	P12-52	Cell	Escherichia Coli (E.Coli)	11.97 nM	PBS containing 1.4 mM MgCl2	27104834

Aptamer Sequence:	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG
The optimal secondary structure in dot-bracket notation:	((((((.((....)).))).))).(((((......)))))
The centroid secondary structure in dot-bracket notation:	((((((.((....)).)))).)).(((((......)))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_831	Description	P12-52
Aptamer chemistry	DNA	Length	40 nt
GC content	77.5%	Molecular weight	12,494.98 Da
Molarity of 1 μg/μl solution	80.03 μM	Number of G-quadruplexes	2
G-Score	19	Function	Aptasensor
Sequence	CCGCCCAGCGGGGGTAGGGCCGGACGTAGGAGGAGCTGCG
Applications	Diagnosis of MNEC-borne pathologies

Type	Detail	Type	Detail
ATCC ID	25922	Cell name	Escherichia Coli (E.Coli)
Category	Bacteria	Organism	N/A
Product type	Preceptrol culture	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Freeze-dried
Antigen expression	N/A	Genes expressed	N/A
Cell description	This strain does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
Application(s)	Bioinformatics; Food testing; Media testing; Quality control

	Interaction ID	917
	Target type	Cell
	Target unique ID	25922
	Activity	11.97 nM
	Binding Conditions/Buffer	PBS containing 1.4 mM MgCl2
	Assay	Binding affinity of selected pool 12 aptamers to E coli ATCC 25922
	PubMed ID	27104834

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_706	DNA	CCGGCCAAGGGTGGGAGGGAGGGGGCCGG	62.50%
Apta_1080	DNA	CTTACGACCCAGGGGGGTGGACAGGCGGGGGTTAGGGGGGTCGTAAG	59.57%
Apta_666	DNA	TCGACCTCGCGCGAGGAGGGTGGAGGGTCGTAGAGCGCGTA	58.54%
Apta_255	DNA	TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT	57.78%
Apta_63	DNA	GACCCGTGGTAGGGTAGGATGGGGTGGTC	57.50%
Apta_150	DNA	CCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGG	57.50%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	57.50%
Apta_648	DNA	GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC	57.50%
Apta_862	DNA	TGTGCCAAAGAGAGTGGTGGGGGGGTGGGCGGAACTCGCG	57.50%
Apta_1094	DNA	GGAATCGTGGGTGGTAGGGTAGGGGATGCA	57.50%
Apta_1189	DNA	GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG	57.50%
Apta_1035	DNA	ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC	56.67%
Apta_1047	DNA	TAGTCCCAAGGCACATAAGGGCCGGAGGTCTAGCGTCAGGGATACAGAGA	56.00%
Apta_488	DNA	CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG	55.81%
Apta_143	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAGTTTCG	55.10%
Apta_14	DNA	AGCGGGGGGGGCTGGGCTGGGTGGG	55.00%
Apta_15	DNA	TGAGGGGGGGGGAGGGCGGGGCTGC	55.00%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	55.00%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	55.00%
Apta_245	DNA	ACAGGGGTGTGGGGACAGGGGTGTGGGG	55.00%

ABOUT