AptaDB: Result

Interaction ID: 810

Aptamer sequence: CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG

Target unique ID: CRL-1739

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_728	cy-apt 20	Cell	carcinoma cell	N/A	20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, and 0.05% [v/v] Tween 20	25248985

Aptamer Sequence:	CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG
The optimal secondary structure in dot-bracket notation:	.....((((......((((.........................))))))))
The centroid secondary structure in dot-bracket notation:	.....(((.......((((.........................)))).)))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_728	Description	cy-apt 20
Aptamer chemistry	DNA	Length	52 nt
GC content	55.8%	Molecular weight	15,856.20 Da
Molarity of 1 μg/μl solution	63.07 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	CGACCCGGCACAAACCCAGAACCATATACACGATCATTAGTCTCCTGGGCCG
Applications	Detection of gastric cancer cell

Type	Detail	Type	Detail
ATCC ID	CRL-1739	Cell name	carcinoma cell
Category	Human cells	Organism	Homo sapiens, human
Product type	N/A	Cell type	N/A
Morphology	epithelial	Tissue	Stomach
Disease	Gastric Adenocarcinoma	Product format	Frozen
Antigen expression	N/A	Genes expressed	N/A
Cell description	N/A
Application(s)	3D cell culture

	Interaction ID	810
	Target type	Cell
	Target unique ID	CRL-1739
	Activity	N/A
	Binding Conditions/Buffer	20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, and 0.05% [v/v] Tween 20
	Assay	The specific binding capacity of aptamer cy-apt 20 to gastric carcinoma cells was also assessed with flow cytometry by varying the concentration of FITC-cy-apt 20
	PubMed ID	25248985

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_663	DNA	CACGCACAGAAGACACCTACAGGGCCAGATCACAATCGGTTAGTGAACTCGTACGGCGCG	61.67%
Apta_1218	DNA	CGACACCACACAGCAGTAAGCACTACAACATAGTGGTCTAGACTATCTCGGGCCGGTG	58.62%
Apta_689	DNA	CCACACAACAGCCTACGTGGATACACCACATACATCCCATAACCCCGTGTCATGTGTCG	55.93%
Apta_221	DNA	GTCCTAGCAGACACTCGGCCATCACGCCCATTGGTTTGCA	55.77%
Apta_868	DNA	CCCAGAAACAGCACCCTTACATCGACTGGACCTTCCGCG	55.77%
Apta_960	DNA	CCCGAGGCACGAGGGGGAACGTATCGTCATGTGCCGGCCA	55.77%
Apta_684	DNA	GGCGGACCAAGGGGACACCACAGATGAATGTACAGTACCATGTTACTGCGCCCGTAGGTG	55.00%
Apta_888	DNA	GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC	55.00%
Apta_1265	DNA	CACGCACACAACAGCCAATAATGTATAACGCTGTCCACTGTGTGGTGTCCCCCGCGTCG	54.24%
Apta_664	DNA	CACGCAACAACACAACATGCCCAGCGCCTGGAACATATCCTATGAGTTAGTCCGCCCACA	53.33%
Apta_993	DNA	AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG	53.33%
Apta_1228	DNA	GGCCACCAAACCACACCGTCGCAACCGCGAGAACCGAAGTAGTGATCATGTCCCTGCGTG	53.33%
Apta_992	DNA	TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG	53.23%
Apta_1172	DNA	GACGCCACTGACTACCCCAGTATCTCTTCAACGCGTATTGAAGTCAGTCGGTCGTCA	52.63%
Apta_1219	DNA	GCGCAGAACCGACAGCAAAACGGAAGTCCACCACGACTATTGGTGCCCAATCTACCGTA	52.54%
Apta_158	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG	51.92%
Apta_339	DNA	CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTGT	51.92%
Apta_735	DNA	AGGCCCCCAGGCTCGGTGGATGCAAACACATGACTATGGGCCCGT	51.92%
Apta_847	DNA	CACTAGGATCTAGGGAAAAATCTCGTAAATACTATACTCCTGAGC	51.92%
Apta_873	DNA	CCACCGAGCCTACCACATGTGACATCCCAGGACATAGCTG	51.92%

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