AptaDB: Result

Interaction ID: 1137

Aptamer sequence: TGCTGTTGTCACCTGCCTCGTCTCCCTCGT

Target unique ID: P09758

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1083	Tac-B1	Protein	TACD2_HUMAN	153 nM	1 mM MgCl2 in PBS Buffer, Binding-20 mM Tris-HCl buffer [pH 7.4], 10 mM NaCl, 1 mM MgCl2, and 0.05% Tween 20	34204006

Aptamer Sequence:	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT
The optimal secondary structure in dot-bracket notation:	..............................
The centroid secondary structure in dot-bracket notation:	..............................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1083	Description	Tac-B1
Aptamer chemistry	DNA	Length	30 nt
GC content	60.0%	Molecular weight	9,042.79 Da
Molarity of 1 μg/μl solution	110.59 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	TGCTGTTGTCACCTGCCTCGTCTCCCTCGT
Applications	Capacity for internalization by cells

Type	Detail	Type	Detail
Uniprot ID	P09758	Protein name	Tumor-associated calcium signal transducer 2 (Cell surface glycoprotein Trop-2) (Membrane component chromosome 1 surface marker 1) (Pancreatic carcinoma marker protein GA733-1)
Gene name(s)	TACSTD2 GA733-1 M1S1 TROP2	Organism	Tumor-associated calcium signal transducer 2 (Cell surface glycoprotein Trop-2) (Membrane component chromosome 1 surface marker 1) (Pancreatic carcinoma marker protein GA733-1)
Status	reviewed in uniprot database	Length	323
Mass	35,709	Activity regulation	N/A
Involvement in disease	Corneal dystrophy, gelatinous drop-like (GDLD)	Catalytic activity	N/A
PDB ID(s)	2MAE;2MVK;2MVL;7E5M;7E5N;7PEE;
Function	May function as a growth factor receptor.

	Interaction ID	1137
	Target type	Protein
	Target unique ID	P09758
	Activity	153 nM
	Binding Conditions/Buffer	1 mM MgCl2 in PBS Buffer, Binding-20 mM Tris-HCl buffer [pH 7.4], 10 mM NaCl, 1 mM MgCl2, and 0.05% Tween 20
	Assay	Evaluation of the internalization of each aptamer obtained via CE-SELEX and hybrid-type SELEX
	PubMed ID	34204006

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_539	DNA	CGTGTTGTCTCTCTGTGTCTCG	63.33%
Apta_540	DNA	ACGTGTTGTCTCTCTGTGTCTCGT	63.33%
Apta_147	DNA	TGCGGTTGTAGTACTCGTGGCCG	60.00%
Apta_538	DNA	GTGTTGTCTCTCTGTGTCTC	60.00%
Apta_541	DNA	CACGTGTTGTCTCTCTGTGTCTCGTG	60.00%
Apta_254	DNA	CCCGCTGTTCTCCCCCCCCCGCTGAGATCCCTGT	58.82%
Apta_542	DNA	GCACGTGTTGTCTCTCTGTGTCTCGTGC	56.67%
Apta_1192	DNA	CTTTCTGTCCTTCCGTCACATCCCACGCATTCTCCACAT	56.41%
Apta_167	DNA	TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG	55.00%
Apta_912	DNA	TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT	55.00%
Apta_392	DNA	GGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	54.55%
Apta_249	DNA	CCCTGTTCCCCCTCCGCCGCTTGACTCTCC	53.33%
Apta_543	DNA	GGCACGTGTTGTCTCTCTGTGTCTCGTGCC	53.33%
Apta_620	DNA	TTCTTTCTTCCCCTTGTTTGTT	53.33%
Apta_1086	DNA	GCAGTTGATCCTTTGGATACCCTGG	53.33%
Apta_1282	DNA	TCCTCTCACACATCTTATCCCCTAGGG	53.33%
Apta_160	DNA	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA	52.50%
Apta_222	DNA	AGCGACTGACGATCTTGAGTAAACCGCTCATCCACGTAGT	52.50%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	52.50%
Apta_966	DNA	GGCCTGGTCTGTTAGCCGGGTAGCAGCCCCGGCACCTATT	52.50%

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