AptaDB: Result

Interaction ID: 164

Aptamer sequence: CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA

Target unique ID: P04183

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_160	TK1_apta37	Protein	KITH_HUMAN	570 pM	PBS	30900869

Aptamer Sequence:	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA
The optimal secondary structure in dot-bracket notation:	(((((((.((....)))))..))))...(((...)))...
The centroid secondary structure in dot-bracket notation:	((((((..((....)).))..))))...(((...)))...

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_160	Description	TK1_apta37
Aptamer chemistry	DNA	Length	40 nt
GC content	57.5%	Molecular weight	12,601.88 Da
Molarity of 1 μg/μl solution	79.35 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA
Applications	Diagnostic assays

Type	Detail	Type	Detail
Uniprot ID	P04183	Protein name	Thymidine kinase, cytosolic (EC 2.7.1.21)
Gene name(s)	TK1	Organism	Thymidine kinase, cytosolic (EC 2.7.1.21)
Status	reviewed in uniprot database	Length	234
Mass	25,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	Reaction=ATP + thymidine = ADP + dTMP + H(+)
PDB ID(s)	1W4R;1XBT;2ORV;2WVJ;
Function	Cell-cycle-regulated enzyme of importance in nucleotide metabolism.

No relevant experimental diagram	Interaction ID	164
	Target type	Protein
	Target unique ID	P04183
	Activity	570 pM
	Binding Conditions/Buffer	PBS
	Assay	N/A
	PubMed ID	30900869

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	57.50%
Apta_1024	DNA	GTCCAGGGTTCCAAGGTGCTTCGTGGAC	57.50%
Apta_1156	DNA	TTCTGGTGTGGTAACTCATGTATCGGTCGTGGGGGTCGTGGCATT	55.56%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	55.00%
Apta_1204	DNA	GGCACGATGTGGCTACATCGATCGCGGTACTGGTG	55.00%
Apta_1127	DNA	TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC	54.90%
Apta_144	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGAGGTAG	54.55%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	54.35%
Apta_395	DNA	AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA	54.00%
Apta_943	DNA	GAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCT	54.00%
Apta_974	DNA	CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC	54.00%
Apta_1045	DNA	GGTGCTCAGGGAACTGTCTGAGGGATCAGGCTTAAGCCTGTCGAGCAGTT	54.00%
Apta_910	DNA	TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA	52.73%
Apta_125	DNA	TGGTGGATGGCGCAGTCGGCGACAA	52.50%
Apta_145	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCGATAGA	52.50%
Apta_161	DNA	GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC	52.50%
Apta_181	DNA	CCGTGTAAGGTTTCAGTGGCTTATCCGAAGTAACCCGAGA	52.50%
Apta_229	DNA	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC	52.50%
Apta_302	DNA	ATCCTGTGAGGAATGCTCATGCATAGCAAGGGCT	52.50%
Apta_497	DNA	GTTCGATTGGATTGTGCCGGAAGTGCTGGCTCGAAC	52.50%

ABOUT