Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1291
Aptamer sequence: CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG
Target unique ID: 5353447
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1229 | BT 5 | Molecule | Brevetoxin 2 | 311 nM | 50 mM Tris, pH 7.5, and 10 mM MgCl2 | 25725463 |
Structure information of aptamer
| Aptamer Sequence: | CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG |
| The optimal secondary structure in dot-bracket notation: | (((((((.........((((........)))).(((((((....))))))))))..)))). |
| The centroid secondary structure in dot-bracket notation: | .................................(((((((....))))))).......... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1229 | Description | BT 5 |
| Aptamer chemistry | DNA | Length | 61 nt |
| GC content | 67.2% | Molecular weight | 19,142.17 Da |
| Molarity of 1 μg/μl solution | 52.24 μM | Number of G-quadruplexes | 3 |
| G-Score | 58 | Function | Aptasensor |
| Sequence | CACGGGCAGAGGGATAGGTTGTTGACGGGGCTGGTGGGTGGGTGCGCTCGCGCTATCGTGG | ||
| Applications | Detection of BTX-2 in food samples | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| PubChem ID | 5353447 | Molecular name | Brevetoxin 2 |
| Molecular formula | C50H70O14 | Molecular weight | 895.1 |
| IUPAC name | 2-[[(21Z)-12-hydroxy-1,3,11,24,31,41,44-heptamethyl-39-oxo-2,6,10,15,19,25,29,34,38,43,47-undecaoxaundecacyclo[26.22.0.03,26.05,24.07,20.09,18.011,16.030,48.033,46.035,44.037,42]pentaconta-21,40-dien-14-yl]methyl]prop-2-enal | InChIKey | LYTCVQQGCSNFJU-KTKRTIGZSA-N |
| LogP | 5.472 | Topological polar surface area | 155.9 |
| Hydrogen bond acceptor | 14 | Hydrogen bond donor | 1 |
Synonym(s): Brevetoxin 2; brevetoxin pbtx-2; 79580-28-2; Brevetoxin T 34; 2-[[(21Z)-12-hydroxy-1,3,11,24,31,41,44-heptamethyl-39-oxo-2,6,10,15,19,25,29,34,38,43,47-undecaoxaundecacyclo[26.22.0.03,26.05,24.07,20.09,18.011,16.030,48.033,46.035,44.037,42]pentaconta-21,40-dien-14-yl]methyl]prop-2-enal; Brevetoxin PbTx 2; GB 2 toxin; T-34 toxin; GB 2 (Gymnodinium); Toxin T-34; T 4-7 toxin; Ptychodiscus brevis toxin 2; GB 2; T 4-7
Activity data
|
No relevant experimental diagram |
Interaction ID | 1291 |
| Target type | Molecule | |
| Target unique ID | 5353447 | |
| Activity | 311 nM | |
| Binding Conditions/Buffer | 50 mM Tris, pH 7.5, and 10 mM MgCl2 |
|
| Assay | N/A |
|
| PubMed ID | 25725463 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_109 | DNA | AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA | |
| Apta_650 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC | |
| Apta_236 | DNA | AGCAGCACAGAGGTCAGATGAGATGGTTGACCGGTTGATGTGATTAAGGAGACGTCGATTCCTATGCGTGCTACCGTGAA | |
| Apta_771 | DNA | GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC | |
| Apta_76 | DNA | CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG | |
| Apta_223 | DNA | CATTGAGATAGCTAGTTGTAGCTGCGTCATAGGCTGGGTTGGGTCTAGTGGTTGGGTGTG | |
| Apta_391 | DNA | ACCGTGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCACGGT | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_940 | DNA | TGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGT | |
| Apta_942 | DNA | AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA | |
| Apta_773 | DNA | GAATTCAGTCGGACAGCGGTGGTGCTTGTGTATGGGGGTGGTTGGTGGGTTTTATGCTGCTGGATGGACGAATATCGTCTCCC | |
| Apta_708 | DNA | CGTACGGAATTCGCTAGCGGGCGGGGGTGCTGGGGGAATGGAGTGCTGCGTGCTGCGGGGATCCGAGCTCCACGTG | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_110 | DNA | AGCAGCACAGAGGTCAGATGGTTAGGAAGGTGAATAGGTACGACTGCAGGTCTATCGCAGCCTATGCGTGCTACCGTGAA | |
| Apta_173 | DNA | AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA | |
| Apta_832 | DNA | CCGGAGGTGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGACCGGAGGGGGGTGAGGTCTGCGGCAGGCTGTGTGGGTGGA | |
| Apta_1117 | DNA | CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG | |
| Apta_79 | DNA | ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT | |
| Apta_282 | DNA | ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT |
DEV TOOL
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