Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1157
Aptamer sequence: CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT
Target unique ID: Q9H0M0
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1101 C3A Protein WWP1_HUMAN 1.9 μM 50 mM Tris/0.05% Tween-20 29518962
Structure information of aptamer
Aptamer Sequence: CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT
The optimal secondary structure in dot-bracket notation: .(((...)))..........((((...((((.(((.....))))))).))))...((((....)))).......
The centroid secondary structure in dot-bracket notation: .((.....))..........((((...((((.(((.....))))))).))))......................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1101 Description C3A
Aptamer chemistry DNA Length 74 nt
GC content 50.0% Molecular weight 22,735.57 Da
Molarity of 1 μg/μl solution 43.98 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence CCGTAATACGACTCACTATAGGTATGTGACGCGCGTCAATCGCTGTCCTACCAAGCTTTGCAGAGAGGATCCTT
Applications Osteoporosis therapeutic
Target information
Type Detail Type Detail
Uniprot ID Q9H0M0 Protein name NEDD4-like E3 ubiquitin-protein ligase WWP1 (EC 2.3.2.26) (Atrophin-1-interacting protein 5) (AIP5) (HECT-type E3 ubiquitin transferase WWP1) (TGIF-interacting ubiquitin ligase 1) (Tiul1) (WW domain-containing protein 1)
Gene name(s) WWP1 Organism NEDD4-like E3 ubiquitin-protein ligase WWP1 (EC 2.3.2.26) (Atrophin-1-interacting protein 5) (AIP5) (HECT-type E3 ubiquitin transferase WWP1) (TGIF-interacting ubiquitin ligase 1) (Tiul1) (WW domain-containing protein 1)
Status reviewed in uniprot database Length 922
Mass 105,202 Activity regulation Activated by NDFIP1- and NDFIP2-binding
Involvement in disease N/A Catalytic activity Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
PDB ID(s)
1ND7;2OP7;5HPS;5HPT;6J1X;6J1Y;
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Activity data
Interaction ID 1157
Target type Protein
Target unique ID Q9H0M0
Activity 1.9 μM
Binding Conditions/Buffer

50 mM Tris/0.05% Tween-20
Assay

Michaelis-Menten plot of two different C3A concentrations for determination of mode of inhibition from representative gel data
PubMed ID 29518962
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_281 DNA CCGTAATACGACTCACTATAGGGGAGCTCGGTACCGAATTCATGTTGGTAGTTGGCTTGTGTTCGTGTGTTAAGCTTTCAGAGAGGATCCTT 65.22%
Apta_952 DNA TCACCTCATACGACTCACTATAGCGGACCCCACTCACTGCTCGGACTCTCCCCCTCCCCGCCTGGCTCGAACAAGCTTGC 56.25%
Apta_573 DNA CGTACAGTGGGCTATATTGCTGACGTAAGAGCTGCTTTCAATGCATTGGCATAGATGTCA 55.41%
Apta_495 DNA GGGAATGGATCCACATCTACGTATTACTGCTGGTGACGAGGGCTAGACGACGTACCTTCACTGCAGACTTGACGAAGCTT 55.00%
Apta_550 DNA GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT 55.00%
Apta_530 DNA ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT 54.05%
Apta_1102 DNA TACGACTCACTATAGGGATCCGTGACTGTACGGGCTCAGTCGTTACTTGAGAGTTGAATTCCCTTTAGTGAGGGTT 53.95%
Apta_1114 DNA TAATACGACTCACTATAGGGAGACCCAAGCCGATTTATTTTGTGCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT 53.95%
Apta_1120 DNA GGGAATGGATCCACATCTACGAATTCGGAATGAGTGCCCTGCAAGCGAGGGCTAGCTTCACTGCAGACTTGACGAAGCTT 53.75%
Apta_953 DNA TCACCTCATACGACTCACTATAGCGGATCCGAGCCGGGGTGTTCTGTTGGCGGGGGCGGTGGGCGGGCTGGCTCGAACAAGCTTGC 53.49%
Apta_950 DNA CACCTAATACGACTCACTATAGCGGATCCGAAGGGGGCGCGAGGTGTAAGGGTGTGGGGTGGTGGGTGGGCCTGGCTCGAACAAGCTTGC 53.33%
Apta_951 DNA CACCTAATACGACTCACTATAGCGGATCCGAGGGGGTAGCGGGTGGGCCGGTGGATGCGGGGCGCCGGCGCCTGGCTCGAACAAGCTTGC 53.33%
Apta_932 DNA ATACGACTCACTATTAGGGATGTCCACCATATAGATCGATTTAAGTCCCTCGTTATTAATGCTAGACTACTGACTACAA 53.16%
Apta_587 DNA GGAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTT 52.94%
Apta_567 DNA CCTAAGGGGGGACTCAGCATTTTGTGCGGGCGGCGCTAACACAATCAGATAGAGCGGGGTT 52.70%
Apta_694 DNA ACTCACTATGGAAGAGATGGCGACATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT 52.70%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 52.70%
Apta_1105 DNA TACGACTCACTATAGGGATCCAATGGCACAGCGCCTGGAACGTACTCTGTACCTGGAATTCCCTTTAGTGAGGGTT 52.63%
Apta_1118 DNA GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT 52.50%
Apta_1119 DNA GGGAATGGATCCACATCTACGAATTCGGTTGTGAAGATTGGGAGCGTCGTGGCTACTTCACTGCAGACTTGACGAAGCTT 52.50%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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