Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1064
Aptamer sequence: TATGGAGAGTCAATGAGTACAGTCTCGTCTCAATAACGGAGCCTCGTCCGTGCCCCCG
Target unique ID: P04640
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_981 OC17 Protein OSTCN_RAT 95.96 nM 2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water 33379043
Structure information of aptamer
Aptamer Sequence: TATGGAGAGTCAATGAGTACAGTCTCGTCTCAATAACGGAGCCTCGTCCGTGCCCCCG
The optimal secondary structure in dot-bracket notation: ......(((...(((((......))))))))....(((((......))))).......
The centroid secondary structure in dot-bracket notation: ............(((((......))))).......(((((......))))).......
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_981 Description OC17
Aptamer chemistry DNA Length 58 nt
GC content 55.2% Molecular weight 17,818.42 Da
Molarity of 1 μg/μl solution 56.12 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Aptasensor
Sequence TATGGAGAGTCAATGAGTACAGTCTCGTCTCAATAACGGAGCCTCGTCCGTGCCCCCG
Applications Binding to target proteins
Target information
Type Detail Type Detail
Uniprot ID P04640 Protein name Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Gene name(s) Bglap Bglap2 Organism Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Status reviewed in uniprot database Length 99
Mass 10,927 Activity regulation N/A
Involvement in disease N/A Catalytic activity N/A
PDB ID(s)
N/A
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Activity data
Interaction ID 1064
Target type Protein
Target unique ID P04640
Activity 95.96 nM
Binding Conditions/Buffer

2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water
Assay

Determination of affinity/dissociation constant (Kd) of from the saturation binding curve
PubMed ID 33379043
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_585 DNA GATCGAGGGCAGCGATAGCTGGGCTAATAAGGTTAGCCCCATCGGTC 56.90%
Apta_992 DNA TCACTGACAGTAGGCCCATACGCACATTCAACAATCGGTAGTCCATACAACCGCGCCCCGTG 56.45%
Apta_1125 DNA TCGGGCGAGTCGTCTGGGGAGGTCAGAACGAAAGGCCCGCATCGTCCTCCC 55.17%
Apta_1283 DNA TACTGATGCAGGGTAATGCTTCTGTGTTTGTTCTTCATCATTAACGGCCGCGTCTGGCGG 55.00%
Apta_278 DNA AGCAGCACAGAGGTCAGATGAGGCCTGGTGATCGTGCCCAGGCCATATGAGCAAGGAACCCCTATGCGTGCTACCGTGAA 53.75%
Apta_486 DNA GGGAGGGATTGGGTGGGCACAGCTCGCTGATAGGTCCTGGTCTCAC 53.45%
Apta_591 DNA CTCTCGGGACGACGGCTGGCACGTTTGGTTCAAGAATGTGGGTGTCGTCCC 53.45%
Apta_639 DNA CTAAGCACAGGGAAACCAGCTAATGAGTTAGGCCTGTCCCCCACG 53.45%
Apta_1126 DNA TCGGGCGAGTCGTCTGCGGGCGGATAGACTACGACACCGCATCGTCCTCCC 53.45%
Apta_1127 DNA TCGGGCGAGTCGTCTGCGAGGCAACAAGCTCGTAATCCGCATCGTCCTCCC 53.45%
Apta_1232 DNA GGCCACACAAACAACATGACAACACGTCTCACATAACGCCCACGTGCTGCCGCCTCATCG 53.33%
Apta_1115 DNA CGTACGGAATTCGCTAGCGGTGACGGACGTGGGGTGCACGAAGGGAGGGGATCCGAGCTCCACGTG 53.03%
Apta_1117 DNA CGTACGGAATTCGCTAGCGGACTGGAGTCTAGACCGGGTAGCTGTGGTGGATCCGAGCTCCACGTG 53.03%
Apta_908 DNA ATACGGGAGCCAACACCAATTCCGCCAAAGTCTGCTAATAAACGGACGCCGGGGAGAGCAGGTGTGACGGAT 52.78%
Apta_61 DNA AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA 52.50%
Apta_105 DNA AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA 52.50%
Apta_105 DNA AGCAGCACAGAGGTCAGATGATGCTCTAGGCGAAAGACTTGGCGTAGAACGACGGGAGGCCCTATGCGTGCTACCGTGAA 52.50%
Apta_175 DNA AGCAGCACAGAGGTCAGATGAGGACTGTCGATAAGTATAATGGCAAGTAGCTCGATACATCCTATGCGTGCTACCGTGAA 52.50%
Apta_176 DNA AGCAGCACAGAGGTCAGATGTATGGTACATTCGAGCGAGCTAAACTAGGATTAAACTCACCCTATGCGTGCTACCGTGAA 52.50%
Apta_1017 DNA TAGGGAATTCGTCGACGGATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGGTCGACGCATGCGCCG 51.90%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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