Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1059
Aptamer sequence: GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC
Target unique ID: Other-41
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_976 | H3.12 | Other | HIV reverse transcriptase (HIV RT) | 69.9 nM | 20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1 mM CaCl2 | 32298104 |
Structure information of aptamer
| Aptamer Sequence: | GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC |
| The optimal secondary structure in dot-bracket notation: | .....(((((((.....(((((((....)))))))......))))))).. |
| The centroid secondary structure in dot-bracket notation: | .....(((((((.....(((((((....)))))))......))))))).. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_976 | Description | H3.12 |
| Aptamer chemistry | DNA | Length | 50 nt |
| GC content | 32.0% | Molecular weight | 15,510.02 Da |
| Molarity of 1 μg/μl solution | 64.47 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC | ||
| Applications | Binding to target proteins | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-41 | Name | HIV reverse transcriptase (HIV RT) |
Activity data
|
No relevant experimental diagram |
Interaction ID | 1059 |
| Target type | Other | |
| Target unique ID | Other-41 | |
| Activity | 69.9 nM | |
| Binding Conditions/Buffer | 20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1 mM CaCl2 |
|
| Assay | N/A |
|
| PubMed ID | 32298104 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_972 | DNA | TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC | |
| Apta_973 | DNA | TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC | |
| Apta_975 | DNA | AGACATAGTACATAACCGATCAATGAACATTTAATTAAATGGTGGTATCC | |
| Apta_970 | DNA | TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC | |
| Apta_977 | DNA | CAATCTGCTAAAACTTAAAGCCTATTAATGAAATTGTTAAGGTGGTATCC | |
| Apta_407 | DNA | GTGAATTGCGTTGAATTATGTTTTTTCAGTCATCACT | |
| Apta_385 | DNA | ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC | |
| Apta_578 | DNA | CACCAAGCGCAGGGAATTACATTGAAGTGTGGGATTGGCT | |
| Apta_929 | DNA | GAGTGGGATGGATAGAGAATAAGTGTGGAGTGGAAGGTGA | |
| Apta_988 | DNA | AAGGATGACTGACGACGATGGGAATGGAGAGGAAGGGGATATAGGTATGTTGGATGTAG | |
| Apta_991 | DNA | AGCAGTGAAGAGGCGAGAGATTTATGAGGGTAGCGTCCACATATGCCAGGATCGTGTTG | |
| Apta_884 | DNA | ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT | |
| Apta_267 | DNA | GAGTGCGTAATGGTACGATTTGGGAAGTGGCTTGGGGTGG | |
| Apta_274 | DNA | GATACTGAGCATCGTACATGATCCCGCAACGGGCAGTATT | |
| Apta_642 | DNA | ATCCAATACCTCAGAACTCAGTTCGAGTCGTAAAGGGGAATCGCA | |
| Apta_974 | DNA | CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC | |
| Apta_986 | DNA | CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG | |
| Apta_1065 | DNA | CCTCGCTATGATGGAGTGCGTTTAGATCAGGGAACGGGTT | |
| Apta_1286 | DNA | CTATACGTATTCTAACTTGTTACGTATTGGTCTCTTATTG | |
| Apta_422 | DNA | GGGAGCTCAGAATAAACGCTCAATTCGGGAATGATTATCAAATTTATGCCCTCTGATTTCGACATGAGGCCCGGATC |
DEV TOOL
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