Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 855
Aptamer sequence: GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC
Target unique ID: HTB-15
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_772 GBM11 Cell human glioblastoma cell line U118-MG 52 nM 25 μM glucose 4 μM yeast tRNA 15 μM BSA 5 mM MgCl2 in PBS (pH = 7.4) 1 hour incubation with cells 23056171
Structure information of aptamer
Aptamer Sequence: GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC
The optimal secondary structure in dot-bracket notation: ...((((((((((((((((.......)))..................)))))))))))))...((((((...))))))...
The centroid secondary structure in dot-bracket notation: ...(((((((((((((...............................)))))))))))))...((((((...))))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_772 Description GBM11
Aptamer chemistry DNA Length 81 nt
GC content 56.8% Molecular weight 25,285.11 Da
Molarity of 1 μg/μl solution 39.55 μM Number of G-quadruplexes 2
G-Score 20 Function Target detection
Sequence GAATTCAGTCGGACAGCGCGGTGTTGGTGGTGGGGTGGTTTGGGAATCTGTTCGGCTGGAGATGGACGAATATCGTCTCCC
Applications Early detection, Targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma
Target information
Type Detail Type Detail
ATCC ID HTB-15 Cell name human glioblastoma cell line U118-MG
Category Human cells Organism Homo sapiens, human
Product type N/A Cell type N/A
Morphology mixed Tissue Brain
Disease Glioblastoma; Grade IV Product format Frozen
Antigen expression Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47 Genes expressed N/A
Cell description U-118 MG is a cell line exhibiting mixed morphology that was isolated in 1966 from malignant gliomas from a 50-year-old, male with Grade IV glioblastoma.
Application(s) 3D cell culture; Neuroscience
Activity data

No relevant experimental diagram

 Interaction ID 855
Target type Cell
Target unique ID HTB-15
Activity 52 nM
Binding Conditions/Buffer

25 μM glucose 4 μM yeast tRNA 15 μM BSA 5 mM MgCl2 in PBS (pH = 7.4) 1 hour incubation with cells
Assay

N/A
PubMed ID 23056171
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_775 DNA GAATTCAGTCGGACAGCGCGGTGTTGGTGGCGGGGGTGGTTGGGGGATTTGGCTGTCCTGAGTGATGGACGAATATCGTCTCCC
85.71%
Apta_773 DNA GAATTCAGTCGGACAGCGGTGGTGCTTGTGTATGGGGGTGGTTGGTGGGTTTTATGCTGCTGGATGGACGAATATCGTCTCCC 79.52%
Apta_769 DNA GAATTCAGTCGGACAGCGAGTTGTTGTTAGGGTGTTGGGTTGGGGTTTTAGGGTCCCTGTCGTCGATGGACGAATATCGTCTCCC 76.47%
Apta_1183 DNA GAATTCAGTCGGACAGCGGCGTGAGTCTTTTGCGGTGGCTCGGGTTCGAATATCTACGACCTTGATGGACGAATATCGTCTCCC 73.81%
Apta_774 DNA GAATTCAGTCGGACAGCGAGGAAAATTTCGTTATTTTCGTTTCCGGGAACTCGGGCATTTAGAGATGGACGAATATCGTCTCCC 71.43%
Apta_771 DNA GAATTCCGTCGGACAGCGGTATGCATGGGAGGTTCTGGAGGGGGTGGGTTGGCGTGCGTTCCAGATGGACGAATATCGTCTCCC 70.24%
Apta_768 DNA GAATTCAGTCGGACAGCGGCACTTGCGACACGTTTGTCGGGTAAATGCGTGTATTTTCTTTTCGATGGACGAATATCGTCTCCC 69.05%
Apta_1182 DNA GAATTCAGTCGGACAGCGTCAGTCGTCCCTTTATGCTTGGTGCTCACCGTCTGAATTTCTTCAGATGGACGAATATCGTCTCCC 69.05%
Apta_767 DNA GAATTCAGTCGGACAGCGACGGTGGGAGCCCCAAATAATTCTTGCGATTATTAGTGTAAGCGGATGGACGAATATCGTCTCCC 68.67%
Apta_1180 DNA GAATTCAGTCGGACAGCGCAATTGTGGTTCTTACCCTATCCCTTGTGTTTGGCGTTCGTTGCGATGGACGAATATCGTCTCCC 68.67%
Apta_1184 DNA GAATTCAGTCGGACAGCGTAATCCCTTGTGTTTGACGTTCGAAGATTATTTCTATCTTTTAGGATGGACGAATATCGTCTCCC 68.67%
Apta_1181 DNA GAATTCAGTCGGACAGCGTAGTAAGAATCTGTTTCTGGTCCTAGTCTTGCTTGTGTTTCCTGATGGACGAATATCGTCTCCC 68.29%
Apta_770 DNA GAATTCAGTCGGACAGCGGCATTTACATTCCATGGGAAATTTCTCAAGCGGTATTTCATTTCGATGGACGAATATCGTCTCCC 66.27%
Apta_1179 DNA GAATTCAGTCGGACAGCGAAGTAGTTTTCCTTCTAACCTAAGAACCCGCGGCAGTTTAATGTAGATGGACGAATACGTCTCCC 63.86%
Apta_67 DNA AGATGCCTGTCGAGCATGCTGTTGTGGTAGGGTTAGGGATGGTAGCGGTTGTAGCTAAACTGCTTTGTCGACGGG 54.32%
Apta_127 DNA GGTATTGAGGGTCGCATCCACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGTGATGGCTCTAACTCTCCTCT 54.32%
Apta_128 DNA GGTATTGAGGGTCGCATCCCGTAGTGTGTTCTTATTCGTGTCTGTGTGTGTTCTGTCGGATGGCTCTAACTCTCCTCT 54.32%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 54.32%
Apta_589 DNA GGGAATTCGAGCTCGGTACCATCTGTGTAAGGGGTAAGGGGTGGGGGTGGGTACGTCTAGCTGCAGGCATGCAAGCTTGG 54.32%
Apta_456 DNA AGCTCCAGAAGATAAATTACAGGTGGGTGGGTGGGCGGTGGAATTTAGCGGCGGAGCTCTGTGTGTGTTAGGGCAACTAGGATACTATGACCCC 54.26%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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