AptaDB: Result

Interaction ID: 377

Aptamer sequence: GGCAGACCAUACGGGAGAGAAACUUGCC

Target unique ID: P25098

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_371	C13.28	Protein	ARBK1_HUMAN	1.2 nM	20 mM Tris (pH 7.0), 50 mM NaCl, 5 mM MgCl2, 2 mM DTT, and 0.1% Lubrol (without nonspecific RNA)	22727813

Aptamer Sequence:	GGCAGACCAUACGGGAGAGAAACUUGCC
The optimal secondary structure in dot-bracket notation:	(((((..................)))))
The centroid secondary structure in dot-bracket notation:	((((....................))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_371	Description	C13.28
Aptamer chemistry	RNA	Length	28 nt
GC content	57.1%	Molecular weight	9,062.51 Da
Molarity of 1 μg/μl solution	110.34 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	GGCAGACCAUACGGGAGAGAAACUUGCC
Applications	Trap a novel conformational state of a protein kinase

Type	Detail	Type	Detail
Uniprot ID	P25098	Protein name	Beta-adrenergic receptor kinase 1 (Beta-ARK-1) (EC 2.7.11.15) (G-protein coupled receptor kinase 2)
Gene name(s)	GRK2 ADRBK1 BARK BARK1	Organism	Beta-adrenergic receptor kinase 1 (Beta-ARK-1) (EC 2.7.11.15) (G-protein coupled receptor kinase 2)
Status	reviewed in uniprot database	Length	689
Mass	79,574	Activity regulation	In contrast to other AGC family kinases, the catalytic activity is solely regulated by the binding of substrates and ligands
Involvement in disease	N/A	Catalytic activity	Reaction=[beta-adrenergic receptor] + ATP = [beta-adrenergic receptor]-phosphate + ADP + H(+)
PDB ID(s)	1BAK;3CIK;3KRW;3KRX;3V5W;4MK0;4PNK;5HE1;5UKK;5UKL;5UUU;5UVC;5WG3;5WG4;5WG5;6C2Y;6U7C;7K7L;7K7Z;7PWD;
Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.

No relevant experimental diagram	Interaction ID	377
	Target type	Protein
	Target unique ID	P25098
	Activity	1.2 nM
	Binding Conditions/Buffer	20 mM Tris (pH 7.0), 50 mM NaCl, 5 mM MgCl2, 2 mM DTT, and 0.1% Lubrol (without nonspecific RNA)
	Assay	N/A
	PubMed ID	22727813

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_372	RNA	GACCAUACGGGAGAGAAACU	71.43%
Apta_373	RNA	CCAUACGGGAGAGAAACU	64.29%
Apta_323	DNA	GGCTATAGCACATGGGTAAAACGAC	60.71%
Apta_323	DNA	GGCTATAGCACATGGGTAAAACGAC	60.71%
Apta_323	DNA	GGCTATAGCACATGGGTAAAACGAC	60.71%
Apta_560	RNA	GGAAGAGAUGGCGACUAAAACGACUUGUCGC	58.06%
Apta_138	RNA	GGAGGUGCUCCGAAAGGAACUCC	57.14%
Apta_1292	RNA	GGCACGUGUAUUACCCUAGUGGUCGACGUGCC	56.25%
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	54.29%
Apta_231	RNA	GGGAGGACGAUGCGGGUGUGGGGCG	53.57%
Apta_630	DNA	GACCAGGGCAAACGGTAGGGAGTGGTC	53.57%
Apta_1167	RNA	GGCGCGAGGAAGGAGGUCUGAGGAGGUCACUGCGCC	52.78%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	52.63%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	52.63%
Apta_633	RNA	GGCAUCGGAAAGUGGGUUGAUGUAAGUAACAGGCGAUGCC	52.50%
Apta_711	RNA	GGCGAUACCAGCCGAAAGGCCCUUGGCAGCGUC	51.52%
Apta_717	RNA	GGCGCAGCCCGCCGAAAGGCACGUGGAACCGUC	51.52%
Apta_343	DNA	AACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGAC	51.28%
Apta_98	RNA	CGAUGCGGUCUCAUGCGUCGAGUGUGAGUUUACCUUCG	50.00%
Apta_360	DNA	GGGAACGCATACGCCGCAGTATTTCCC	50.00%

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