PS14

The dose-dependent cell viability of PS14-treated cells are as follows: 5 µM, 94.4 ± 2.66%; 10 µM, 87.31 ± 4%; 15 µM, 87.25 ± 7.21%; 20 µM, 84.44 ± 3.89% and 25 µM, 89.57± 2.39% (Figure 1).

The dose-dependent cell viability of PS14-treated cells are as follows: 5 µM, 94.4 ± 2.66%; 10 µM, 87.31 ± 4%; 15 µM, 87.25 ± 7.21%; 20 µM, 84.44 ± 3.89% and 25 µM, 89.57± 2.39% (Figure 1).

The dose-dependent cell viability of PS14-treated cells are as follows: 5 µM, 94.4 ± 2.66%; 10 µM, 87.31 ± 4%; 15 µM, 87.25 ± 7.21%; 20 µM, 84.44 ± 3.89% and 25 µM, 89.57± 2.39% (Figure 1).

The dose-dependent cell viability of PS14-treated cells are as follows: 5 µM, 94.4 ± 2.66%; 10 µM, 87.31 ± 4%; 15 µM, 87.25 ± 7.21%; 20 µM, 84.44 ± 3.89% and 25 µM, 89.57± 2.39% (Figure 1).

The dose-dependent cell viability of PS14-treated cells are as follows: 5 µM, 94.4 ± 2.66%; 10 µM, 87.31 ± 4%; 15 µM, 87.25 ± 7.21%; 20 µM, 84.44 ± 3.89% and 25 µM, 89.57± 2.39% (Figure 1).

The hatching rate of PS14 exposed group was 97.53 ± 1.9% at 5 µM followed by 93.47 ± 1.5% at 10 µM, 91.78 ± 1.7% at 15 µM, 89.43 ± 1.9% at 20 µM and 89.14 ± 1.5% at 25 µM.

The hatching rate of PS14 exposed group was 97.53 ± 1.9% at 5 µM followed by 93.47 ± 1.5% at 10 µM, 91.78 ± 1.7% at 15 µM, 89.43 ± 1.9% at 20 µM and 89.14 ± 1.5% at 25 µM.

The hatching rate of PS14 exposed group was 97.53 ± 1.9% at 5 µM followed by 93.47 ± 1.5% at 10 µM, 91.78 ± 1.7% at 15 µM, 89.43 ± 1.9% at 20 µM and 89.14 ± 1.5% at 25 µM.

The hatching rate of PS14 exposed group was 97.53 ± 1.9% at 5 µM followed by 93.47 ± 1.5% at 10 µM, 91.78 ± 1.7% at 15 µM, 89.43 ± 1.9% at 20 µM and 89.14 ± 1.5% at 25 µM.

The hatching rate of PS14 exposed group was 97.53 ± 1.9% at 5 µM followed by 93.47 ± 1.5% at 10 µM, 91.78 ± 1.7% at 15 µM, 89.43 ± 1.9% at 20 µM and 89.14 ± 1.5% at 25 µM.

Table 1: PS14 5 µM, 24 h, Percentage of Inhibition = 21.32 ± 3.2 *

Table 1: PS14 10 µM, 24 h, Percentage of Inhibition = 27.25 ± 1.89 *

Table 1: PS14 15 µM, 24 h, Percentage of Inhibition = 34.37 ± 2.45 *

Table 1: PS14 20 µM, 24 h, Percentage of Inhibition = 48.70 ± 2.1 *

Table 1: PS14 25 µM, 24 h, Percentage of Inhibition = 59.75 ± 1.7 *

Table 1: PS14 5 µM, 48 h, Percentage of Inhibition = 19.22 ± 1.5 *

Table 1: PS14 10 µM, 48 h, Percentage of Inhibition = 25.35 ± 0.92 *

Table 1: PS14 15 µM, 48 h, Percentage of Inhibition = 39.93 ± 3.24 *

Table 1: PS14 20 µM, 48 h, Percentage of Inhibition = 58.60 ± 1.7 *

Table 1: PS14 25 µM, 48 h, Percentage of Inhibition = 57.41 ± 1.8 *

Table 1: PS14 5 µM, 24 h, Viable Cell Count Percentage = 79.35 ± 0.94 *

Table 1: PS14 10 µM, 24 h, Viable Cell Count Percentage = 73.24 ± 4.25 *

Table 1: PS14 15 µM, 24 h, Viable Cell Count Percentage = 61.52 ± 3.8 *

Table 1: PS14 20 µM, 24 h, Viable Cell Count Percentage = 54.75 ± 4 *

Table 1: PS14 25 µM, 24 h, Viable Cell Count Percentage = 41.25 ± 5 *

Table 1: PS14 5 µM, 24 h, Dead Cell Count Percentage = 23.86 ± 0.98 *

Table 1: PS14 10 µM, 24 h, Dead Cell Count Percentage = 28.78 ± 2.3 *

Table 1: PS14 15 µM, 24 h, Dead Cell Count Percentage = 36.48 ± 0.80 *

Table 1: PS14 20 µM, 24 h, Dead Cell Count Percentage = 48.25 ± 1.32 *

Table 1: PS14 25 µM, 24 h, Dead Cell Count Percentage = 66.32 ± 2.45 *

Table 1: PS14 5 µM, 24 h, Percentage of LDH Release = 9.74 ± 1.54

Table 1: PS14 10 µM, 24 h, Percentage of LDH Release = 19.31 ± 1.09 *

Table 1: PS14 15 µM, 24 h, Percentage of LDH Release = 34.31 ± 1.6 *

Table 1: PS14 20 µM, 24 h, Percentage of LDH Release = 45.56 ± 2 *

Table 1: PS14 25 µM, 24 h, Percentage of LDH Release = 69.05 ± 1.6 *

Results of the proteinase inhibition assay showed that PS14, dose-dependently and significantly increased the proteinase inhibition, as: 25.45 ± 1% (5 µM), 39.21 ± 1.5% (10 µM), 49.10 ± 1.89% (15 µM), 69 ± 1.3% (20 µM) and 77 ± 2.8% (25 µM).

Results of the proteinase inhibition assay showed that PS14, dose-dependently and significantly increased the proteinase inhibition, as: 25.45 ± 1% (5 µM), 39.21 ± 1.5% (10 µM), 49.10 ± 1.89% (15 µM), 69 ± 1.3% (20 µM) and 77 ± 2.8% (25 µM).

Results of the proteinase inhibition assay showed that PS14, dose-dependently and significantly increased the proteinase inhibition, as: 25.45 ± 1% (5 µM), 39.21 ± 1.5% (10 µM), 49.10 ± 1.89% (15 µM), 69 ± 1.3% (20 µM) and 77 ± 2.8% (25 µM).

Results of the proteinase inhibition assay showed that PS14, dose-dependently and significantly increased the proteinase inhibition, as: 25.45 ± 1% (5 µM), 39.21 ± 1.5% (10 µM), 49.10 ± 1.89% (15 µM), 69 ± 1.3% (20 µM) and 77 ± 2.8% (25 µM).

Results of the proteinase inhibition assay showed that PS14, dose-dependently and significantly increased the proteinase inhibition, as: 25.45 ± 1% (5 µM), 39.21 ± 1.5% (10 µM), 49.10 ± 1.89% (15 µM), 69 ± 1.3% (20 µM) and 77 ± 2.8% (25 µM).

PS14 challenge showed a dose-dependent inhibition at 5 µM (19.71 ± 1%) followed by the other higher concentration and the results are significantly different from the control (p < 0.05).

The concentration of PS14 which inhibits 50% of cell proliferation (IC50) at 24 h was 21 µM, and at 48 h, it was 18.75 µM.

The concentration of PS14 which inhibits 50% of cell proliferation (IC50) at 24 h was 21 µM, and at 48 h, it was 18.75 µM.