International Journal of Molecular Sciences · 2024
Sequence evidence
KKCHFWPFPW
KKCHFWPFPW
Experimental observations
Section 2.2: SPR analysis. The affinity (equilibrium dissociation constant, KD) between the KKCH peptide and the ACE2 protein was 6.24 ± 0.35 × 10^−7 M (association (Ka) = 1.54 ± 0.15 × 10^5 1/Ms; dissociation (Kd) = 9.58 ± 0.76 × 10^−2 1/s).
Section 2.2: SPR analysis. The affinity KD between the SARS-CoV-2 S1 protein and the ACE2 protein was 1.54 ± 0.55 × 10^−7 M (Ka = 2.24 ± 0.18 × 10^5 1/Ms, Kd = 3.46 ± 0.22 × 10^−2 1/s).
Section 2.3: ELISA. At concentration of 10 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 6.80%.
Section 2.3: ELISA. At concentration of 90 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 10.93%.
Section 2.3: ELISA. At concentration of 180 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 17.01%.
Section 2.3: ELISA. At concentration of 360 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 34.00%.
Section 2.3: ELISA. At concentration of 720 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 89.67%.
Section 2.3: ELISA. At concentration of 1440 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 94.12%.
Section 2.3: ELISA. According to the ELISA inhibition curve, the IC50 value was determined to be 413.2 μM.
Section 2.4: CCK-8 assay. At KKCH concentration of 40 μM, the viability rate of BEAS-2B cells was 99.80%.
Section 2.4: CCK-8 assay. At KKCH concentration of 80 μM, the viability rate of BEAS-2B cells was 99.77%.
Section 2.4: CCK-8 assay. At KKCH concentration of 160 μM, the viability rate of BEAS-2B cells was 99.37%.
Section 2.4: CCK-8 assay. At KKCH concentration of 240 μM, the viability rate of BEAS-2B cells was 99.63%.
Section 2.4: CCK-8 assay. At KKCH concentration of 320 μM, the viability rate of BEAS-2B cells was 98.07%.
Section 2.4: CCK-8 assay. At KKCH concentration of 400 μM, the viability rate of BEAS-2B cells was 95.87%.
Section 2.4: CCK-8 assay. At KKCH concentration of 480 μM, the viability rate of BEAS-2B cells was 82.95%.
Section 2.4: CCK-8 assay. At KKCH concentration of 40 μM, the viability rate of HEK293T cells was 98.28%.
Section 2.4: CCK-8 assay. At KKCH concentration of 80 μM, the viability rate of HEK293T cells was 95.28%.
Section 2.4: CCK-8 assay. At KKCH concentration of 160 μM, the viability rate of HEK293T cells was 94.74%.
Section 2.4: CCK-8 assay. At KKCH concentration of 240 μM, the viability rate of HEK293T cells was 92.39%.
Section 2.4: CCK-8 assay. At KKCH concentration of 320 μM, the viability rate of HEK293T cells was 90.77%.
Section 2.4: CCK-8 assay. At KKCH concentration of 400 μM, the viability rate of HEK293T cells was 92.69%.
Section 2.4: CCK-8 assay. At KKCH concentration of 480 μM, the viability rate of HEK293T cells was 81.66%.
Section 2.6: Pseudovirus assay. At 20 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 21.74%.
Section 2.6: Pseudovirus assay. At 60 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 31.50%.
Section 2.6: Pseudovirus assay. At 180 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 31.30%.
Section 2.6: Pseudovirus assay. At 360 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 42.30%.
Section 2.6: Pseudovirus assay. At 450 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 63.15%.
Section 2.6: Pseudovirus assay. The IC50 was calculated to be 398.1 μM.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 40 μM, the survival rate of Vero cells was 97.81%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 80 μM, the survival rate of Vero cells was 96.97%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 160 μM, the survival rate of Vero cells was 98.23%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 240 μM, the survival rate of Vero cells was 96.21%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 320 μM, the survival rate of Vero cells was 95.85%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 400 μM, the survival rate of Vero cells was 92.64%.
Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 480 μM, the survival rate of Vero cells was 90.44%.
Section 2.6: Live virus neutralization. At 10 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 4.97%.
Section 2.6: Live virus neutralization. At 25 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 11.32%.
Section 2.6: Live virus neutralization. At 50 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 15.40%.
Section 2.6: Live virus neutralization. At 100 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 17.91%.
Section 2.6: Live virus neutralization. At 150 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 22.41%.
Section 2.6: Live virus neutralization. At 200 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 32.01%.
Section 2.6: Live virus neutralization. At 350 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 38.31%.
Section 2.6: Live virus neutralization. At 450 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 54.80%.
Section 2.6: Live virus neutralization. The IC50 was calculated to be 462.4 μM.
Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.
