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Primary-literature peptidemedium evidence

PEPDIA-PLP-001493

KKCH

Aliases: KKCH

Normalized sequence

KKCHFWPFPW

10 amino acids

Release PepDiana-V1-20260620

45

Activity records

1

Source papers

45

Positive

0

Negative / threshold

Versioned scientific descriptors

Physicochemical properties and standard representations

calculated sequence and structure

Reported properties

No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.

Computed properties

physchem-20260620

Molecular weight

1375.6399 Da

Molecular formula

C71H90N16O11S

Theoretical pI

9.3145

Net charge at pH 7

1.8354

GRAVY

-0.79

Instability index

66.87

Aromaticity

0.4

Topological polar surface area

419.94 Å²

cLogP

1.8609

Hydrogen-bond donors

15

Hydrogen-bond acceptors

15

Rotatable bonds

36

Method: PepDiana sequence/structure descriptor pipeline

Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6

Calculated: 2026-06-20T11:01:38.358971+00:00

Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200

Sequence representations

representations-20260620

ProForma 2.0

validated simple linear

KKCHFWPFPW

HELM 2.0

validated simple linear

PEPTIDE1{K.K.C.H.F.W.P.F.P.W}$$$$V2.0

Canonical SMILES

validated

NCCCCC(N)C(=O)NC(CCCCN)C(=O)NC(CS)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(Cc1ccccc1)C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)N1CCCC1C(=O)NC(Cc1ccccc1)C(=O)N1CCCC1C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)O

Isomeric SMILES

validated

NCCCC[C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O

Paper-level observations

Experimental evidence grouped by activity

Antiviral 24 records

PMC11277083

24 observations

AntiviralGeneral AntiviralPositive observation

Section 2.2: SPR analysis. The affinity (equilibrium dissociation constant, KD) between the KKCH peptide and the ACE2 protein was 6.24 ± 0.35 × 10^−7 M (association (Ka) = 1.54 ± 0.15 × 10^5 1/Ms; dissociation (Kd) = 9.58 ± 0.76 × 10^−2 1/s).

Mention PMC11277083::PEP000001PMC11277083text

Measure: other
Value: 6.24 ± 0.35 × 10^−7 M (KD)
Target: ACE2
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.2: SPR analysis. The affinity KD between the SARS-CoV-2 S1 protein and the ACE2 protein was 1.54 ± 0.55 × 10^−7 M (Ka = 2.24 ± 0.18 × 10^5 1/Ms, Kd = 3.46 ± 0.22 × 10^−2 1/s).

Mention PMC11277083::PEP000001PMC11277083text

Measure: other
Value: 1.54 ± 0.55 × 10^−7 M (KD)
Target: ACE2
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 10 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 6.80%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 6.80%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 90 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 10.93%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 10.93%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 180 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 17.01%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 17.01%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 360 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 34.00%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 34.00%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 720 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 89.67%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 89.67%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. At concentration of 1440 μM, the inhibitory rate of KKCH on S1-RBD/ACE2 was 94.12%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 94.12%
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.3: ELISA. According to the ELISA inhibition curve, the IC50 value was determined to be 413.2 μM.

Mention PMC11277083::PEP000001PMC11277083text

Measure: other
Value: 413.2 μM
Target: ACE2 / S1-RBD
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. At 20 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 21.74%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 21.74%
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. At 60 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 31.50%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 31.50%
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. At 180 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 31.30%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 31.30%
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. At 360 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 42.30%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 42.30%
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. At 450 μM, the inhibition rate of KKCH on SARS-CoV-2 pseudovirus was 63.15%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 63.15%
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Pseudovirus assay. The IC50 was calculated to be 398.1 μM.

Mention PMC11277083::PEP000001PMC11277083text

Measure: other
Value: 398.1 μM
Target: 293T
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 10 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 4.97%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 4.97%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 25 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 11.32%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 11.32%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 50 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 15.40%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 15.40%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 100 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 17.91%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 17.91%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 150 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 22.41%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 22.41%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 200 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 32.01%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 32.01%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 350 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 38.31%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 38.31%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. At 450 μM, the inhibitory rate of KKCH against SARS-CoV-2 was 54.80%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: percent_inhibition
Value: 54.80%
Target: Vero
Assay: Not reported by source

Source publication

AntiviralGeneral AntiviralPositive observation

Section 2.6: Live virus neutralization. The IC50 was calculated to be 462.4 μM.

Mention PMC11277083::PEP000001PMC11277083text

Measure: other
Value: 462.4 μM
Target: Vero
Assay: Not reported by source

Source publication

Toxicity 21 records

PMC11277083

21 observations

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 40 μM, the viability rate of BEAS-2B cells was 99.80%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 99.80%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 80 μM, the viability rate of BEAS-2B cells was 99.77%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 99.77%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 160 μM, the viability rate of BEAS-2B cells was 99.37%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 99.37%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 240 μM, the viability rate of BEAS-2B cells was 99.63%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 99.63%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 320 μM, the viability rate of BEAS-2B cells was 98.07%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 98.07%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 400 μM, the viability rate of BEAS-2B cells was 95.87%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 95.87%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 480 μM, the viability rate of BEAS-2B cells was 82.95%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 82.95%
Target: BEAS-2B
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 40 μM, the viability rate of HEK293T cells was 98.28%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 98.28%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 80 μM, the viability rate of HEK293T cells was 95.28%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 95.28%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 160 μM, the viability rate of HEK293T cells was 94.74%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 94.74%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 240 μM, the viability rate of HEK293T cells was 92.39%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 92.39%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 320 μM, the viability rate of HEK293T cells was 90.77%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 90.77%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 400 μM, the viability rate of HEK293T cells was 92.69%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 92.69%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.4: CCK-8 assay. At KKCH concentration of 480 μM, the viability rate of HEK293T cells was 81.66%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 81.66%
Target: HEK293T
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 40 μM, the survival rate of Vero cells was 97.81%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 97.81%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 80 μM, the survival rate of Vero cells was 96.97%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 96.97%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 160 μM, the survival rate of Vero cells was 98.23%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 98.23%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 240 μM, the survival rate of Vero cells was 96.21%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 96.21%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 320 μM, the survival rate of Vero cells was 95.85%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 95.85%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 400 μM, the survival rate of Vero cells was 92.64%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 92.64%
Target: Vero
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 2.6: CCK-8 assay on Vero cells. At KKCH concentration of 480 μM, the survival rate of Vero cells was 90.44%.

Mention PMC11277083::PEP000001PMC11277083text

Measure: cell_viability
Value: 90.44%
Target: Vero
Assay: Not reported by source

Source publication

Citation and release provenance

Cite this identity as PepDiana record PEPDIA-PLP-001493, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.

Record updated: 2026-06-22T07:14:26.229247+00:00