AptaDB: Result

Interaction ID: 947

Aptamer sequence: CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG

Target unique ID: Other-9

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_861	HMBA	Other	6X His-tag	4.2 nM	1X DPBS without Ca and Mg (pH 7.1-7.5), 20 °C	22916953

Aptamer Sequence:	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG
The optimal secondary structure in dot-bracket notation:	(((((((((......((((...))))......)).)))))))
The centroid secondary structure in dot-bracket notation:	(((((((((.......................)).)))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_861	Description	HMBA
Aptamer chemistry	DNA	Length	42 nt
GC content	57.1%	Molecular weight	12,990.29 Da
Molarity of 1 μg/μl solution	76.98 μM	Number of G-quadruplexes	2
G-Score	21	Function	Target detection
Sequence	CAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACGCAACCTG
Applications	Direct and universal quantitation of recombinant proteins

Type	Detail	Type	Detail
Target ID	Other-9	Name	6X His-tag

	Interaction ID	947
	Target type	Other
	Target unique ID	Other-9
	Activity	4.2 nM
	Binding Conditions/Buffer	1X DPBS without Ca and Mg (pH 7.1-7.5), 20 °C
	Assay	Direct quantification of recombinant protein from cell lysate
	PubMed ID	22916953

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_858	DNA	GGCTTCAGGTTGGTCTGGTTGGGTTTGGCTCCTGTGTACG	71.43%
Apta_857	DNA	GCTATGGGTGGTCTGGTTGGGATTGGCCCCGGGAGCTGGC	64.29%
Apta_70	DNA	AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT	61.90%
Apta_75	DNA	CACTGGTAGGTTGGTGTGGTTGGGGCCAGTG	59.52%
Apta_1237	DNA	GGGGTTGTGTGGGGAGTTTGTCCTGTTGTG	59.52%
Apta_491	DNA	TTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCT	57.14%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	57.14%
Apta_239	DNA	CCCAGATTACTGTGGAGTGGTTGTCTGCGAATCCTTCGTCCACCCAATAG	56.00%
Apta_882	DNA	AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG	56.00%
Apta_484	DNA	CAGGGAGGGACAGGGCGGGAATTTGTATGATGGTTCGTTTGCTCG	55.56%
Apta_41	DNA	CGGTCGCTCCGTGTGGCTTGGGTTGGGTGTGGCAGTGAC	54.76%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	54.76%
Apta_148	DNA	CCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGG	54.76%
Apta_260	DNA	GACGTTAAGCTAGCAGGTGTTAGGTCCCGTGGTGATGAAT	54.76%
Apta_266	DNA	TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT	54.76%
Apta_393	DNA	AGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCT	54.76%
Apta_565	DNA	GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	54.76%
Apta_851	DNA	CCATGTTTTGTAGGTGTAATAGGTCATGTTAGGGTTTCTG	54.76%
Apta_986	DNA	CGTGTATCCCCTGTGTGTTTGTACTCGGCTACTGTATCCG	54.76%
Apta_1205	DNA	GGCAGGTGGTGTTGGTTGGTTGTGCGTGGAGTTGG	54.76%

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