Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 928
Aptamer sequence: TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_842 Apt-7 Cell Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) 17.11 nM 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 29756774
Structure information of aptamer
Aptamer Sequence: TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC
The optimal secondary structure in dot-bracket notation: ....((((((((((....((((.((((..((((((...))))))..)))).))))...))))).........)))))...
The centroid secondary structure in dot-bracket notation: ....((((((((((.(..((((.((((..((((((...))))))..)))).)))).).))))).........)))))...
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_842 Description Apt-7
Aptamer chemistry DNA Length 80 nt
GC content 55.0% Molecular weight 24,832.82 Da
Molarity of 1 μg/μl solution 40.27 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted binding
Sequence TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC
Applications Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Target information
Type Detail Type Detail
ATCC ID BAA-2587-PACK Cell name Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Category Bacteria Organism N/A
Product type Reporter-labeled bacterium Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli.
Application(s) Assay development; Food testing; High-throughput screening
Activity data
Interaction ID 928
Target type Cell
Target unique ID BAA-2587-PACK
Activity 17.11 nM
Binding Conditions/Buffer

1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2
Assay

Histogram of the percent gated fluorescence intensity above library background for individual aptamers
PubMed ID 29756774
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_841 DNA TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC 76.25%
Apta_843 DNA TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC 75.00%
Apta_844 DNA TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC 72.50%
Apta_836 DNA TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_838 DNA TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_840 DNA TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC 71.25%
Apta_837 DNA TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC 70.00%
Apta_839 DNA TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC 67.50%
Apta_656 DNA GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 53.75%
Apta_1235 DNA TGTACCGTCTGAGCGATTCGTACGATCGGGTCGGGGTCTATTTTAGGGCCATTTGGCAGCCAGTCAGTGTTAAGGAGTGC 53.75%
Apta_657 DNA CCTGCACCTCGTCCAGCATGCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 53.41%
Apta_607 DNA TGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTC 52.50%
Apta_650 DNA GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 52.50%
Apta_654 DNA GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTG 52.50%
Apta_109 DNA AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA 51.25%
Apta_109 DNA AGCAGCACAGAGGTCAGATGTGCGACCACCTGGATAGACGAGGGTAGTAAGGAGTGTGTTCCTATGCGTGCTACCGTGA 51.25%
Apta_140 DNA GGCAGGAAGACAAACACGATGGGGGGGTATGATTTGATGTGGTTGTTGCATGATCGTGGTCTGTGGTGCTGT 51.25%
Apta_405 DNA TCTAGCCCACTGCATAGAGGTAGCCACGTATGTGGGTGTTACCCATTCGTAGCTACCGGTG 51.25%
Apta_530 DNA ATCCGTCACACCTGCTCTGTGTACGCCCGTATTTACGTTCCTAGCAATTGCTATGTGGTGTTGGCTCCCGTAT 51.25%
Apta_568 DNA CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT 51.25%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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