Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 924
Aptamer sequence: TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC
Target unique ID: BAA-2587-PACK
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_838 Apt-3 Cell Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) 16.44 nM 1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2 29756774
Structure information of aptamer
Aptamer Sequence: TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC
The optimal secondary structure in dot-bracket notation: ...((((..(((...(((((((.......))))))).)))....))))(((((.((((........)))).)))))....
The centroid secondary structure in dot-bracket notation: .........((....(((((((.......)))))))..))........................................
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_838 Description Apt-3
Aptamer chemistry DNA Length 80 nt
GC content 60.0% Molecular weight 24,928.90 Da
Molarity of 1 μg/μl solution 40.11 μM Number of G-quadruplexes 2
G-Score 21 Function Targeted binding
Sequence TGAGCCCAAGCCCTGGTATGTAGTAATGGTGCGTACAGGCGACGGGGTCCAGGCTGGAGGGGCAGGTCTACTTTGGGATC
Applications Binding to Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Target information
Type Detail Type Detail
ATCC ID BAA-2587-PACK Cell name Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7)
Category Bacteria Organism N/A
Product type Reporter-labeled bacterium Cell type N/A
Morphology N/A Tissue N/A
Disease N/A Product format Frozen
Antigen expression N/A Genes expressed N/A
Cell description A kit containing reporter-labeled Escherichia coli designed to distinguish control strain cross-contamination from true contamination in test samples. This product is appropriate for use as a positive control in quality control assays for E. coli.
Application(s) Assay development; Food testing; High-throughput screening
Activity data
Interaction ID 924
Target type Cell
Target unique ID BAA-2587-PACK
Activity 16.44 nM
Binding Conditions/Buffer

1× BB, 50 mmol/L Tris-HCl (pH 7.4), 5 mmol/L KCl, 100 mmol/L NaCl, and 1 mmol/L MgCl2
Assay

Histogram of the percent gated fluorescence intensity above library background for individual aptamers
PubMed ID 29756774
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_837 DNA TGAGCCCAAGCCCTGGTATGTGATCGGTGACGAGGGTGCGGGGCGGGGGGTGAGGCACAGGGCAGGTCTACTTTGGGATC 75.00%
Apta_840 DNA TGAGCCCAAGCCCTGGTATGCGGATAACGAGGTATTCACGACTGGTCGTCAGGTATGGTTGGCAGGTCTACTTTGGGATC 75.00%
Apta_836 DNA TGAGCCCAAGCCCTGGTATGTTACAGTATGCTACCTCTACTTGAAGGTTGGTCGACGCGGGGCAGGTCTACTTTGGGATC 73.75%
Apta_841 DNA TGAGCCCAAGCCCTGGTATGGTGTGCCTGTCGTTGTATTGGTCGGTAGGGATCGGAGTGGGGCAGGTCTACTTTGGGATC 72.50%
Apta_844 DNA TGAGCCCAAGCCCTGGTATGTTGCGGGAGTCTAGCGGGCCACACTTTTATAGGTTCGCAGGGCAGGTCTACTTTGGGATC 72.50%
Apta_842 DNA TGAGCCCAAGCCCTGGTATGTGTGGGGTCCTGGATTATGTTTAGCGTCTTTCGCAGTGGGGGCAGGTCTACTTTGGGATC 71.25%
Apta_843 DNA TGAGCCCAAGCCCTGGTATGTCACATCCGGGTTCTTTGCGAACGCGTTTCCGCAGTCTCAGGCAGGTCTACTTTGGGATC 71.25%
Apta_839 DNA TGAGCCCAAGCCCTGGTATGAGCCCACGGAACACTGGTCGCGCCCACTGGTTTCTATATTGGCAGGTCTACTTTGGGATC 68.75%
Apta_568 DNA CCCAGCAGCACTGGTAGTGAGGCAGTTCACCGGTGGGGCGGTGAGTTTGGCTGCTATTTAT 55.00%
Apta_661 DNA GGACAACATAGGAAAAAGGCTCTGCTACCGGATCCCTGTTGTATGGGCATATCTGTTGAT 53.75%
Apta_76 DNA CAGCGCTAGGGCTTTTAGCGTAATGGGTAGGGTGGTGCGGTGCAGATATCGGAATTGGTG 52.50%
Apta_336 DNA CATGCTTCCCCAGGGAGATGACCGGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTGG 52.50%
Apta_508 DNA GCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCT 52.50%
Apta_656 DNA GCACACCGATGGCGGTCCTGTTTTACGGTAGCTTATATGCAGGGGGTGTGCGTGGGGAGGTGCGGTTGC 52.50%
Apta_1220 DNA TGGCGACAAGAAGACGTACAAACACGCACCAGGCCGGAGTGGAGTATTCTGAGGTCGG 52.50%
Apta_68 DNA AGATGCCTGTCGAGCATGCTGTGAATAGGTAGGGTCGGATGGGCTACGGTGTAGCTAAACTGCTTTGTCGACGGG 51.25%
Apta_524 DNA ATCCGTCACACCTGCTCTGGCATTAGTATTCCATAGCCGGCCAAGTCTATGTAGTGGTGTTGGCTCCCGTAT 51.25%
Apta_533 DNA ATACGGGAGCCAACACCAAAGTAAGATCATCACCCGGACGCGGACATAATAGGAGAGCAGGTGTGACGGAT 51.25%
Apta_670 DNA TTGCCTAACCGTGAAGGTAAAACGATTTAGTCAAACGTGGGAGGGCGGTGGTGTTGACTGATCGATTTTATTCCA 51.25%
Apta_888 DNA GGCGACCCCCGGGCTACCAGACAATGTACGCAGCAAGAGTGACGGTCGTACCTCGGAGTC 51.25%

ABOUT

AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

HELP CENTER

>Contact us
>LMMD Group
>School of Pharmacy

DEV TOOL
Page last updated at 2022-05-25 10:01:57 (Asia/Shanghai) | You are visitor No. 43982
Copyright © 2019-2020 Laboratory of Molecular Modeling and Design, School of Pharmacy, East China University of Science and Technology. All rights reserved.