AptaDB: Result

Interaction ID: 895

Aptamer sequence: GGGTACGTATTTGGTTTATAGATAGTAA

Target unique ID: 43895

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_809	short arm	Cell	Escherichia coli(E.coli)O157:H7	110 nM	30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA	22166202

Aptamer Sequence:	GGGTACGTATTTGGTTTATAGATAGTAA
The optimal secondary structure in dot-bracket notation:	...(((.((((((.....))))))))).
The centroid secondary structure in dot-bracket notation:	...(((.((((((.....))))))))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_809	Description	short arm
Aptamer chemistry	DNA	Length	28 nt
GC content	32.1%	Molecular weight	8,712.64 Da
Molarity of 1 μg/μl solution	114.78 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Aptasensor
Sequence	GGGTACGTATTTGGTTTATAGATAGTAA
Applications	Diagnostic ligand against the pathogen-related food borne illness

Type	Detail	Type	Detail
ATCC ID	43895	Cell name	Escherichia coli(E.coli) O157:H7
Category	Bacteria	Organism	N/A
Product type	N/A	Cell type	N/A
Morphology	N/A	Tissue	N/A
Disease	N/A	Product format	Freeze-dried
Antigen expression	N/A	Genes expressed	N/A
Cell description	Produces Shiga-like toxins I and II. Quality control strain for Difco products.
Application(s)	Media testing; Quality control

	Interaction ID	895
	Target type	Cell
	Target unique ID	43895
	Activity	110 nM
	Binding Conditions/Buffer	30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA
	Assay	Comparison of binding ability of aptamer I-1 and its truncated aptamers to O157:H7 LPS versus K12 LPS
	PubMed ID	22166202

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	57.14%
Apta_186	DNA	AGGAACGCTTTAGGTCTGAGATCTCGGAAT	56.67%
Apta_216	DNA	TGGTTGGTTTTTGGTTTTTTTTTTTGGTTT	56.67%
Apta_1238	DNA	GGTTTTGTGTTTGTGTTTTGTGGTTGTTGT	56.67%
Apta_1240	DNA	GGAGTAGTGGGGATTGTGGTGTATGTTGTC	56.67%
Apta_916	DNA	GGCGTATGCAGTTTTGTAGGGTTATCAAGGCTTGACGATT	55.00%
Apta_196	DNA	AGGAAGGCTTTAGGTCTGAGATCTC	53.57%
Apta_328	DNA	GGGTTATATTACTCGGCCGGTGTAA	53.57%
Apta_757	DNA	GGTGGTGGTGGTTGTGGTGGTGG	53.57%
Apta_184	DNA	AGGAAGGCTTTAGGTCTGAGATCTCGGAAT	53.33%
Apta_190	DNA	AGGAACCCTTTAGGTCTGAGATCTCGGAAT	53.33%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	53.33%
Apta_1236	DNA	GGACTGTTGTGGTATTATTTTTGGTTGTGC	53.33%
Apta_1239	DNA	GGTGGATGGGGATGCTTTGTTAGTTTGTGC	53.33%
Apta_1244	DNA	GGAGATGTCAGTTGTGTGTGTGGTTATGGC	53.33%
Apta_195	DNA	AGGAAGGCTTTAGGGGTCTGAGATCTCGGAAT	53.12%
Apta_338	DNA	GGGCGCGTTCTTCGTGGTTACTTTTAGTCCCG	53.12%
Apta_443	DNA	GGCCATGACGATGTCGTTACGTAGATGCAGAGACTCCTAA	52.50%
Apta_445	DNA	AGCCATGACGATGTCGTTACGTAGATGTAGAGACTCCTAA	52.50%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	52.50%

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