AptaDB: Result

Interaction ID: 328

Aptamer sequence: ACCCUGAUGGUAGACGCCGGGGUG

Target unique ID: P15692

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_319	NX-178	Protein	VEGFA_HUMAN	2.4 nM	PBS: 10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl and 2.7 mM KCl (pH 7.4) with 0.01 % human serum albumin	9383475

Aptamer Sequence:	ACCCUGAUGGUAGACGCCGGGGUG
The optimal secondary structure in dot-bracket notation:	((((((.((.....)).)))))).
The centroid secondary structure in dot-bracket notation:	((((((.((.....)).)))))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_319	Description	NX-178
Aptamer chemistry	RNA	Length	24 nt
GC content	66.7%	Molecular weight	8,002.61 Da
Molarity of 1 μg/μl solution	124.96 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	ACCCUGAUGGUAGACGCCGGGGUG
Applications	Binds to VPF/VEGF with high affinity and specificity

Type	Detail	Type	Detail
Uniprot ID	P15692	Protein name	Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Gene name(s)	VEGFA VEGF	Organism	Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Status	reviewed in uniprot database	Length	232
Mass	27,042	Activity regulation	N/A
Involvement in disease	Microvascular complications of diabetes 1 (MVCD1)	Catalytic activity	N/A
PDB ID(s)	1BJ1;1CZ8;1FLT;1KAT;1KMX;1MJV;1MKG;1MKK;1QTY;1TZH;1TZI;1VGH;1VPF;1VPP;2FJG;2FJH;2QR0;2VGH;2VPF;3BDY;3P9W;3QTK;3S1B;3S1K;3V2A;4DEQ;4GLN;4GLS;4KZN;4QAF;4WPB;4ZFF;5DN2;5FV1;5FV2;5HHC;5HHD;5O4E;5T89;6BFT;6D3O;6T9D;6V7K;6Z13;6Z3F;6ZBR;6ZCD;6ZFL;7KEZ;7KF0;7KF1;7LL8;7LL9;
Function	Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth.

No relevant experimental diagram	Interaction ID	328
	Target type	Protein
	Target unique ID	P15692
	Activity	2.4 nM
	Binding Conditions/Buffer	PBS: 10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl and 2.7 mM KCl (pH 7.4) with 0.01 % human serum albumin
	Assay	N/A
	PubMed ID	9383475

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_855	RNA	UCACUGUUAUCCGAUAGCAGCGCGGGAUGA	60.00%
Apta_927	RNA	UUACAAUGAGGUGGGACGCGGGUUGUGGUG	60.00%
Apta_342	RNA	GCGACUGGUUACCCGGUCG	54.17%
Apta_924	RNA	GCCUUUUCGGGAUACGCGGAGGGUGGGCAA	53.33%
Apta_13	DNA	AGGGGGGGGGGAGGGGTCGGGGTGG	52.00%
Apta_27	DNA	AGGGGGAGGGGTGGGGCCGGGCAGA	52.00%
Apta_63	DNA	GACCCGTGGTAGGGTAGGATGGGGTGGTC	51.72%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	50.00%
Apta_245	DNA	ACAGGGGTGTGGGGACAGGGGTGTGGGG	50.00%
Apta_378	RNA	UGCUCUUAGAAUGGGACUAAGACACGGGAC	50.00%
Apta_14	DNA	AGCGGGGGGGGCTGGGCTGGGTGGG	48.00%
Apta_28	DNA	AGTGGGGGGGGTGGAGCCGGGTAGA	48.00%
Apta_29	DNA	AGAGGGGGGGGACGGCCCGGGTAGA	48.00%
Apta_126	DNA	TCCTCATATAGAGTGCGGGGCGTGT	48.00%
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	47.73%
Apta_1166	RNA	GAUGUAACUGAAUGAAAUGGUGAAGGACGGGUCCAGUAGGCUGC	47.73%
Apta_172	RNA	GGAGACAUCCCUCUUGUUGGUGGUGCCGUGUGGAUGUCUC	47.50%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	47.37%
Apta_848	RNA	CACCACGAUCACGGUUUCCCUCGCAGGUAAGGUGUAGA	47.37%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	47.37%

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