Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1099
Aptamer sequence: TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT
Target unique ID: Other-44
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1014 | G-NB3 | Other | Activated protein C (APC) | 1.6 nM | 1X PBS | 32486960 |
Structure information of aptamer
| Aptamer Sequence: | TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT |
| The optimal secondary structure in dot-bracket notation: | .................((((.((......)).))))... |
| The centroid secondary structure in dot-bracket notation: | .................(((..((......))..)))... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1014 | Description | G-NB3 |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 67.5% | Molecular weight | 12,617.04 Da |
| Molarity of 1 μg/μl solution | 79.26 μM | Number of G-quadruplexes | 2 |
| G-Score | 20 | Function | Targeted therapy |
| Sequence | TGGGGGTTGGGTGGATAGGCTGGCGTCGGGGCAGGTCAGT | ||
| Applications | G-NB3 inhibits the inactivation of activated cofactors V and VIII with IC50 values of 11.6 and 13.1 nM | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-44 | Name | Activated protein C (APC) |
Activity data
 |
Interaction ID | 1099 |
| Target type | Other | |
| Target unique ID | Other-44 | |
| Activity | 1.6 nM | |
| Binding Conditions/Buffer | 1X PBS |
|
| Assay | Influence of APC aptamers on the anticoagulant activity of endogenously generated APC in human plasma. Pooled normal citrated human plasma was spiked with rabbit thrombomodulin (2 U/mL) and increasing concentrations of APC aptamers. Subsequently, thrombin formation was measured using the CAT method |
|
| PubMed ID | 32486960 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_565 | DNA | GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG | |
| Apta_942 | DNA | AGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGA | |
| Apta_486 | DNA | GGGAGGGATTGGGTGGGCACAGCTCGCTGATAGGTCCTGGTCTCAC | |
| Apta_1186 | DNA | AGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT | |
| Apta_10 | DNA | GGGGGGGGTGGCTGGGCGGGGTGGG | |
| Apta_255 | DNA | TAGCGATACTGCGTGGGTTGGGGCGGGTAGGGCCAGCAGTCTCGT | |
| Apta_746 | DNA | AGAGTGGGGGGGTGGGTGGATTTGACAGGTGGCATGCTGGAGAGT | |
| Apta_1085 | DNA | TGGGGTGGTGGTGTGGGTGGGGGTTTGTTC | |
| Apta_1185 | DNA | CAGGGGGTTTTGGTGGGGGGGGCTGGGTTGTCTTGGGGGTGGGCT | |
| Apta_488 | DNA | CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG | |
| Apta_13 | DNA | AGGGGGGGGGGAGGGGTCGGGGTGG | |
| Apta_18 | DNA | TGGGGGGTTGGACGGGCGGGGCTGC | |
| Apta_25 | DNA | TGTGGGGGTGGTTGGGCGGGGCTGC | |
| Apta_27 | DNA | AGGGGGAGGGGTGGGGCCGGGCAGA | |
| Apta_69 | DNA | AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT | |
| Apta_70 | DNA | AGGTCGTAGGTTGGTGTGGTTGGGGCGACCT | |
| Apta_73 | DNA | GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC | |
| Apta_263 | DNA | AGAGGGGAGGGTCGGGTATCGGCGTGTTCGGGGGATCTGC | |
| Apta_266 | DNA | TGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCT | |
| Apta_478 | DNA | CTTCACTTTCGTGGACGGTGGCGTCGGGGCGGTTTGTATG |
DEV TOOL
 |
 |
 |
 |