AptaDB: Result

Interaction ID: 413

Aptamer sequence: GTGAATTGCGTTGAATTATGTTTTTTCAGTCATCACT

Target unique ID: P51654

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_407	G12msi	Protein	GPC3_HUMAN	0.17 nM	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL bovine serum albumin	33080969

Aptamer Sequence:	GTGAATTGCGTTGAATTATGTTTTTTCAGTCATCACT
The optimal secondary structure in dot-bracket notation:	((((..((..(((((.........))))).)))))).
The centroid secondary structure in dot-bracket notation:	((((.......((((.........))))....)))).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_407	Description	G12msi
Aptamer chemistry	DNA	Length	37 nt
GC content	32.4%	Molecular weight	11,365.33 Da
Molarity of 1 μg/μl solution	87.99 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	GTGAATTGCGTTGAATTATGTTTTTTCAGTCATCACT
Applications	Binding to G12msi

Type	Detail	Type	Detail
Uniprot ID	P51654	Protein name	Glypican-3 (GTR2-2) (Intestinal protein OCI-5) (MXR7) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Gene name(s)	GPC3 OCI5	Organism	Glypican-3 (GTR2-2) (Intestinal protein OCI-5) (MXR7) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Status	reviewed in uniprot database	Length	580
Mass	65,563	Activity regulation	N/A
Involvement in disease	Simpson-Golabi-Behmel syndrome 1 (SGBS1)	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Cell surface proteoglycan that bears heparan sulfate.

	Interaction ID	413
	Target type	Protein
	Target unique ID	P51654
	Activity	0.17 nM
	Binding Conditions/Buffer	4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL bovine serum albumin
	Assay	Etermination of binding affinity of G12msi aptamers to GPC3 protein. E
	PubMed ID	33080969

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1176	DNA	AGTATGATATCGGTGTTTATGGTGTCTGTCTTCATACT	57.89%
Apta_987	DNA	GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC	57.45%
Apta_564	DNA	GGGGACTGTTGTGGTATTATTTTTGGTTGTGCAGT	56.76%
Apta_1285	DNA	AGGAACCTTCTCTTCCTCTATTTTTTAATTCTGTCTACCCT	56.10%
Apta_976	DNA	GATGAATATCGCAATGAAATTATATATGTATAATTGGAACGGTGGTATCC	56.00%
Apta_519	DNA	GTATTGCGGAGGAAGGTTTTTAACCTTCGGGG	54.05%
Apta_1236	DNA	GGACTGTTGTGGTATTATTTTTGGTTGTGC	54.05%
Apta_1238	DNA	GGTTTTGTGTTTGTGTTTTGTGGTTGTTGT	54.05%
Apta_1247	DNA	GGTGGTTGCTGTTGTTTGTGGTTGGTGTGC	54.05%
Apta_646	DNA	TGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC	52.63%
Apta_183	DNA	TAGCACTGTGCGGCGCGTATTGTTCTAATCTGATTACATT	52.50%
Apta_220	DNA	ATCAACTCACCGTAGGTCTAGTGAGAACTTCAAGTCTACT	52.50%
Apta_507	DNA	GGCGGGGCTACGAAGTAGTGATTTTTTCCGATGGCCCGTG	52.50%
Apta_912	DNA	TAGTATGGACTTGTCTTCTTGTCAGAGTCTGGCAGCACGT	52.50%
Apta_1260	DNA	GAAAGGGACGTTCCAAGTTCGTATAAGCAGTCCTGTGCGT	52.50%
Apta_972	DNA	TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC	52.00%
Apta_69	DNA	AGCGAGTAGGTTGGTGTGGTTGGGGCTCGCT	51.35%
Apta_72	DNA	AGTCAGTAGGTTGGTGTGGTTGGGGCTGACT	51.35%
Apta_214	DNA	AGGAAGGCTTTAGGTTTCCTAAAGCCTTCC	51.35%
Apta_216	DNA	TGGTTGGTTTTTGGTTTTTTTTTTTGGTTT	51.35%

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