AptaDB: Result

Interaction ID: 292

Aptamer sequence: ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA

Target unique ID: P0DOF8

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_283	HA68	Protein	M2_I72A8	7.1 nM	50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour	23077124

Aptamer Sequence:	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA
The optimal secondary structure in dot-bracket notation:	.....(((((.....)))))....................
The centroid secondary structure in dot-bracket notation:	.....(((((.....)))))....................

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_283	Description	HA68
Aptamer chemistry	DNA	Length	40 nt
GC content	50.0%	Molecular weight	12,397.75 Da
Molarity of 1 μg/μl solution	80.66 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Target detection
Sequence	ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA
Applications	Be used in rapid detection and typing protocols for fifield applications

Type	Detail	Type	Detail
Uniprot ID	P0DOF8	Protein name	Matrix protein 2 (Proton channel protein M2)
Gene name(s)	M	Organism	Matrix protein 2 (Proton channel protein M2)
Status	reviewed in uniprot database	Length	97
Mass	11,186	Activity regulation	The M2 protein from most influenza A strains is inhibited by amantadine and rimantadine, resulting in viral uncoating incapacity
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry.

	Interaction ID	292
	Target type	Protein
	Target unique ID	P0DOF8
	Activity	7.1 nM
	Binding Conditions/Buffer	50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour
	Assay	The dissociation constant (Kd) of each DNA aptamer was used to describe the strength of the binding affinity between aptamer and recombinant swH3 protein. The Kd was calculated from the dot blot chemiluminescence intensity based on a nonlinear regression equation
	PubMed ID	23077124

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	57.50%
Apta_286	DNA	TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG	57.50%
Apta_284	DNA	CACTCCGTCACTTTAGTGGATCTTTATAAAACCGATGCTG	55.00%
Apta_472	DNA	CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA	55.00%
Apta_287	DNA	CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA	54.90%
Apta_644	DNA	TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA	53.33%
Apta_421	DNA	ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA	53.06%
Apta_571	DNA	ACCTGCGATAGGTAGTTTGTTTCTGTTGCCCTAGCGTCCGTCGTAACAAGTCA	52.83%
Apta_338	DNA	GGGCGCGTTCTTCGTGGTTACTTTTAGTCCCG	52.50%
Apta_503	DNA	ACTTTTGAATGTGGCAACAAATTCGACAGG	52.50%
Apta_675	DNA	ACTGTCCGTCGGGTTTAGGGTGGCGTTCGG	52.50%
Apta_1036	DNA	ATACCAGTCTATTCAATTGGGCCCGTCCGTATGGTGGG	52.50%
Apta_1018	DNA	ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG	52.17%
Apta_335	DNA	ACCGGGCGTACACCGTCGCGGCACATGTCTGAATGCGTTTAGTCTCTGTG	52.00%
Apta_819	DNA	GCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTAT	51.11%
Apta_1278	DNA	CCATGATTCAACTTTACTGGTCTTGTCTTGGCTAGTCGTGTGTCATTCCCTAAGG	50.91%
Apta_1274	DNA	GACTTCGTCGTCTTTAGACTAGCAGTTAGGGTTTACGATTGTGGAGTTAGCCCCCAGGG	50.85%
Apta_7	DNA	CCAGTCTCCCGTTTACCGCGCCTACACATGTCTGAATGCC	50.00%
Apta_48	DNA	ACTGGGCAGATTAACGGTCCTATATTTTCGGTTCTGGAAA	50.00%
Apta_49	DNA	CTTCCATGTTGAGGCGTTTATACCAGTTATCATCGGACTA	50.00%

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