Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 441
Aptamer sequence: CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA
Target unique ID: Q08830
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_435 | 85A | Protein | FGL1_HUMAN | 6 nM | 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6 | 18763762 |
Structure information of aptamer
| Aptamer Sequence: | CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA |
| The optimal secondary structure in dot-bracket notation: | ...(((.(((((((..((....))..))))))).)))...((((..(((..((.......))..)))....))))............... |
| The centroid secondary structure in dot-bracket notation: | ...(((.(((((((..((....))..))))))).)))..................................................... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_435 | Description | 85A |
| Aptamer chemistry | DNA | Length | 90 nt |
| GC content | 55.6% | Molecular weight | 27,523.61 Da |
| Molarity of 1 μg/μl solution | 36.33 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target recognition |
| Sequence | CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA | ||
| Applications | Recognition of other glycoproducts | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | Q08830 | Protein name | Fibrinogen-like protein 1 (HP-041) (Hepassocin) (HPS) (Hepatocyte-derived fibrinogen-related protein 1) (HFREP-1) (Liver fibrinogen-related protein 1) (LFIRE-1) |
| Gene name(s) | FGL1 HFREP1 | Organism | Fibrinogen-like protein 1 (HP-041) (Hepassocin) (HPS) (Hepatocyte-derived fibrinogen-related protein 1) (HFREP-1) (Liver fibrinogen-related protein 1) (LFIRE-1) |
| Status | reviewed in uniprot database | Length | 312 |
| Mass | 36,379 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. |
Activity data
 |
Interaction ID | 441 |
| Target type | Protein | |
| Target unique ID | Q08830 | |
| Activity | 6 nM | |
| Binding Conditions/Buffer | 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6 |
|
| Assay | Binding curves of B-TTP-, TTP-, and M-TTP-derived 85A with fibrinogen (left), deglycosylated fibrinogen (middle), and periodated fibrinogen (right) |
|
| PubMed ID | 18763762 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_799 | DNA | GTTCGTGGTGTGCTGGATGTCCCGTGCACGGCGCCACGTTTTCCTCTCATCCCCGATGACACATCCAGCAGCACGA | |
| Apta_797 | DNA | GTTCGTGGTGTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAGCAGCACGA | |
| Apta_798 | DNA | GTTCGTGGTGTGCTGGATGTCGGAGATGGTCCGCTTCGTTCAACTACTACGCATTTTGACACATCCAGCAGCACGA | |
| Apta_558 | DNA | CCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCA | |
| Apta_860 | DNA | CACGGATCCTGACAAGGATGTGTGCGTTGTCGAGACCTGCGACCGGAACACTACACTGTGTGGGATGGATTTCTTTACAGTTGTGTGCAGCTCCGTCCGACTCTTCCTAGCTATGGTTCGATCAAGA | |
| Apta_1073 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1075 | DNA | CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1111 | DNA | GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC | |
| Apta_1113 | DNA | CCGCCCAAATCCCTAAGAGCACAAACACCAAACACAACCACCCCAACCAGACACACTACACACGCA | |
| Apta_1234 | DNA | TGTACCGTCTGAGCGATTCGTACTGTGGGCACCAATCGTACCCAATACTTGCGAATCAGCCAGTCAGTGTTAAGGAGTGC | |
| Apta_704 | DNA | GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA | |
| Apta_321 | DNA | AACCGCCCAAATCCCTAAGAGTCGGACTGCAACCTATGCTATCGTTGATGTCTGTCCAAGCAACACAGACACACTACACACGCACA | |
| Apta_1074 | DNA | CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT | |
| Apta_1076 | DNA | CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT | |
| Apta_1031 | DNA | GGAGACCGCTTAAGCTTAAAGGAGCGGTTACGAATGCGATGACTTAGGTACCATTGCACTACGGTACCTAAACAGTTCCTTTGGATCCGAATTCGCCGC | |
| Apta_582 | DNA | GTACCAGCTTATTCAATTGGGCGAAGGAACATACGGCAGTTTATGGCCGCTATCGAGATAGACTATCATCTCAACGTCTTCTAGATAGTATGTTCATCAG | |
| Apta_83 | DNA | GGCTTTACACTTTATGCTTCCGTAAAAACACAAACACACGCACATACATAAAATAACAATTTCACACAGGAAACAG | |
| Apta_369 | DNA | GGCTGTTGTGAGCCTCCTCCCAGAGGGAAGACTTTAGGTTCGGTTCACGTCCCGCTTATTCTTACTCCC | |
| Apta_461 | DNA | CGCTCGGCTTCACGAGATTCGTGTCGTTGTGTCCTGTACCCGCCTTGACCAGTCACTCTAGAGCATCCGGACTG | |
| Apta_523 | DNA | ATCCGTCACACCTGCTCTGACGCTGGGGTCGACCCGGAGAAATGCATTCCCCTGTGGTGTTGGCTCCCGTAT |
DEV TOOL
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