Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 860
Aptamer sequence: GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG
Target unique ID: CRL-5800
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_777 | ADE2 | Cell | Lung Adenocarcinoma (H23) | 208 nM | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes | 23082117 |
Structure information of aptamer
| Aptamer Sequence: | GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG |
| The optimal secondary structure in dot-bracket notation: | (((......))).....((....)).................. |
| The centroid secondary structure in dot-bracket notation: | (((......))).....((....)).................. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_777 | Description | ADE2 |
| Aptamer chemistry | DNA | Length | 43 nt |
| GC content | 53.5% | Molecular weight | 12,981.37 Da |
| Molarity of 1 μg/μl solution | 77.03 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG | ||
| Applications | Early detection, Targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-5800 | Cell name | Lung Adenocarcinoma (H23) |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | N/A |
| Morphology | epithelial | Tissue | Lung |
| Disease | Adenocarcinoma; Non-small cell lung cancer | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | Myc+; src+; abl+; erb+; ras+; sis- |
| Cell description | N/A |
|
|
| Application(s) | 3D cell culture; Cancer research; High-throughput screening; Toxicology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 860 |
| Target type | Cell | |
| Target unique ID | CRL-5800 | |
| Activity | 208 nM | |
| Binding Conditions/Buffer | 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes |
|
| Assay | N/A |
|
| PubMed ID | 23082117 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1060 | DNA | AGCAATATTGCCCGCGCGGGGCCAACTGTCCTCACATGA | |
| Apta_1228 | DNA | GGCCACCAAACCACACCGTCGCAACCGCGAGAACCGAAGTAGTGATCATGTCCCTGCGTG | |
| Apta_167 | DNA | TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG | |
| Apta_513 | DNA | TAGGGAAAACCAACCGCCCAGAACTGCAGCATAAC | |
| Apta_448 | DNA | TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC | |
| Apta_254 | DNA | CCCGCTGTTCTCCCCCCCCCGCTGAGATCCCTGT | |
| Apta_822 | DNA | TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGATG | |
| Apta_687 | DNA | GGGACAGCGGAGGTCTCCCACCCACCGGCCCCTGCGGCACACCAACCTGTATGGATGCG | |
| Apta_796 | DNA | GTGCTGGATGTCACCTCCACTCACCACCTCTTCTCCGCTCCTCGTCATGACACATCCAG | |
| Apta_1072 | DNA | TTGAAGTGACTCCCACCCCGCCCCACCGTCCTTGCCACCGTTCCGCCATAGCAGGT | |
| Apta_688 | DNA | GCCATGACAACCAGAGGTACCCCCGCCCACCAGCCCCGAAGTCTGTCAGCCTAGTTGTTG | |
| Apta_993 | DNA | AATCCGACCACGAGAGCAGACCGACCACAGCATTATCTAAACCCATTAGTCTAGTCCTGG | |
| Apta_34 | DNA | TGTCACAATTTCCCCCCTCGCACATTTTGCAAGTAACTGA | |
| Apta_158 | DNA | CACAGGCTACGGCACGTAGAGCATCACCATGATCCTGTG | |
| Apta_159 | DNA | GCGTGCAGCATGACCACCGATGTCACACTTAATGAGAAT | |
| Apta_161 | DNA | GTGTGCAGATCTGATTGCCTGCGTCAACCTACATACAAC | |
| Apta_249 | DNA | CCCTGTTCCCCCTCCGCCGCTTGACTCTCC | |
| Apta_472 | DNA | CCCCCACATTCAAATGGCCCCCCTACTCAATTACTGCACA | |
| Apta_872 | DNA | ACCGCCTTCCACCGTTCTCCACCACCCCTCAAACAACCCT | |
| Apta_875 | DNA | GCCGCGACCATACAACGCAAACAACACGCCTGTACGCTTG |
DEV TOOL
 |
 |
 |
 |