Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 300
Aptamer sequence: AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC
Target unique ID: P10148
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_291 | ADR22 | Protein | CCL2_MOUSE | 370 pM | 110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20 | 11591377 |
Structure information of aptamer
| Aptamer Sequence: | AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC |
| The optimal secondary structure in dot-bracket notation: | ........(((....(((((...)))))....))).... |
| The centroid secondary structure in dot-bracket notation: | ........(((....(((((...)))))....))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_291 | Description | ADR22 |
| Aptamer chemistry | DNA | Length | 39 nt |
| GC content | 51.3% | Molecular weight | 12,071.56 Da |
| Molarity of 1 μg/μl solution | 82.84 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted binding |
| Sequence | AAAAATCTAGGCGCTGAAGCCCCGCTTCCTTACCTTCAC | ||
| Applications | Recognise MCP-1 in different ways | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P10148 | Protein name | C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2) |
| Gene name(s) | Ccl2 Je Mcp1 Scya2 | Organism | C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2) |
| Status | reviewed in uniprot database | Length | 148 |
| Mass | 16,326 | Activity regulation | N/A |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). |
Activity data
 |
Interaction ID | 300 |
| Target type | Protein | |
| Target unique ID | P10148 | |
| Activity | 370 pM | |
| Binding Conditions/Buffer | 110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20 |
|
| Assay | Aptamer inhibition of heparin binding. Binding measurements were carried out by surface plasmon resonance using a Biacore 2000 instrument at 25 ℃ |
|
| PubMed ID | 11591377 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_419 | DNA | ATCAGGGCTAAAGAGTGCAGAGTTACTTAG | |
| Apta_421 | DNA | ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA | |
| Apta_439 | DNA | TAACACTCGATTCTCCTAGCCCGCTAGAAATTCCCCTCCC | |
| Apta_470 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCTCAT | |
| Apta_214 | DNA | AGGAAGGCTTTAGGTTTCCTAAAGCCTTCC | |
| Apta_1060 | DNA | AGCAATATTGCCCGCGCGGGGCCAACTGTCCTCACATGA | |
| Apta_644 | DNA | TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA | |
| Apta_847 | DNA | CACTAGGATCTAGGGAAAAATCTCGTAAATACTATACTCCTGAGC | |
| Apta_1162 | DNA | AGAGGCTACAGCGATAAGTCGACATTGCTGACCGTACCTAGTAATACGT | |
| Apta_238 | DNA | CACACATTCCCGTCCTCGATACGTCTAGGCTTAGTGCCACTTGCTTAATC | |
| Apta_290 | DNA | GAACTCACCGGGAAGAAGCCCGTTCCGTCACAGACATGTT | |
| Apta_395 | DNA | AATCCACGTATCGACTAGCTCAAACCAGCCTGATAGAGCCATTCGCTTTA | |
| Apta_448 | DNA | TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC | |
| Apta_468 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAT | |
| Apta_469 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACACCCCAG | |
| Apta_471 | DNA | CTGTAATTGGCGTATGTAACCCAGGCACCAAACGCCCCAG | |
| Apta_891 | DNA | GGAGACCGTACCATCTGTTCGTGGAAGCGCTTTGCTCGTCCATTAGCCTTGTGCTCGTGC | |
| Apta_1063 | DNA | AACTTATCGGAGCGTGATTTCCGTCTCGCCGCTTTCCTTT | |
| Apta_910 | DNA | TCACGTGCATGATAGACGGCGAAGCCGTCGAGTTGCTGTGTGCCGATGCACGTGA | |
| Apta_777 | DNA | GAGCCCTATCTCACACCGCACCCGCAAACTATCATCCTACATG |
DEV TOOL
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