Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 295
Aptamer sequence: TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG
Target unique ID: P0DOF8
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_286 | HA2b | Protein | M2_I72A8 | 3.7 nM | 50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour | 23077124 |
Structure information of aptamer
| Aptamer Sequence: | TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG |
| The optimal secondary structure in dot-bracket notation: | ..........((.(((.(((..........)))))).)). |
| The centroid secondary structure in dot-bracket notation: | .............(((.(((..........)))))).... |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_286 | Description | HA2b |
| Aptamer chemistry | DNA | Length | 40 nt |
| GC content | 45.0% | Molecular weight | 12,445.80 Da |
| Molarity of 1 μg/μl solution | 80.35 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Target detection |
| Sequence | TACTCCGTCACTTTAGTGGATATAACAACCTCCCTATGGG | ||
| Applications | Be used in rapid detection and typing protocols for fifield applications | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Uniprot ID | P0DOF8 | Protein name | Matrix protein 2 (Proton channel protein M2) |
| Gene name(s) | M | Organism | Matrix protein 2 (Proton channel protein M2) |
| Status | reviewed in uniprot database | Length | 97 |
| Mass | 11,186 | Activity regulation | The M2 protein from most influenza A strains is inhibited by amantadine and rimantadine, resulting in viral uncoating incapacity |
| Involvement in disease | N/A | Catalytic activity | N/A |
| PDB ID(s) | N/A |
 |
Function | Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. |
Activity data
 |
Interaction ID | 295 |
| Target type | Protein | |
| Target unique ID | P0DOF8 | |
| Activity | 3.7 nM | |
| Binding Conditions/Buffer | 50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour |
|
| Assay | The dissociation constant (Kd) of each DNA aptamer was used to describe the strength of the binding affinity between aptamer and recombinant swH3 protein. The Kd was calculated from the dot blot chemiluminescence intensity based on a nonlinear regression equation |
|
| PubMed ID | 23077124 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_284 | DNA | CACTCCGTCACTTTAGTGGATCTTTATAAAACCGATGCTG | |
| Apta_283 | DNA | ACTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGA | |
| Apta_640 | DNA | CAATGGACCTATTCGAGTACTGAATAGAACAGTCGGCGCTCTGGG | |
| Apta_167 | DNA | TGCTCCTTGGCCTTAGCCCTGGAACCATCAATCCTCTTCG | |
| Apta_285 | DNA | GCCACTCCGTCACTTTAGTGGTTTTTTTTATAAATGCTTC | |
| Apta_822 | DNA | TAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGATG | |
| Apta_1084 | DNA | TTCCCTCCTCTGTTGTTCCCCCCTCCTCTC | |
| Apta_1269 | DNA | TAGCACACAACCCTTTGATTGGGATATCTCATCCCTCG | |
| Apta_1282 | DNA | TCCTCTCACACATCTTATCCCCTAGGG | |
| Apta_1018 | DNA | ATCCCTGCAGTTTGGTAGGGTGCGAGCAACATCAGGCACCTGCAGG | |
| Apta_185 | DNA | ACCAAGGCTTTAGGTCTGAGATCTCCCAAT | |
| Apta_434 | DNA | ACACGACGGGAATACTGACTCTCCCCCATGT | |
| Apta_580 | DNA | ATGCGGGTATATGGTGCGAGAAAACAAGGGGCATACTGGA | |
| Apta_915 | DNA | ACTTCGGCTAGTTAGGGGGTAGTTTAGATCGTCTCTACAT | |
| Apta_729 | DNA | AACACCGTGGAGGATAGTTCGGTGGCTGTTCAGGGTCTCCTCCCGGTG | |
| Apta_514 | DNA | ACTCAGCACGCGTGTAGGGAAAACCAACCGCCCACAAGCTGCATGCAACG | |
| Apta_882 | DNA | AATAGTCTGGTTCGGTGCTTGGTGCGATGTGTTAGTCAGACTCTTGTGTG | |
| Apta_1273 | DNA | TCCCTCCTGTTGGTTTGGGATGGTGGCGACTATCTGATTGTTTGACTCCCCGTAGGGG | |
| Apta_735 | DNA | AGGCCCCCAGGCTCGGTGGATGCAAACACATGACTATGGGCCCGT | |
| Apta_820 | DNA | TCCCACGATCTCATTAGTCTGTGGATAAGCGTGGGACGTCTATGA |
DEV TOOL
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