AptaDB: Result

Interaction ID: 236

Aptamer sequence: GGGAGGACGAUGCGGGUGUGGGGCG

Target unique ID: P06734

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_231	ICEL2.2	Protein	FCER2_HUMAN	35 nM	PBS containing 1 mM MgCl2 (pH 7.4)	8683119

Aptamer Sequence:	GGGAGGACGAUGCGGGUGUGGGGCG
The optimal secondary structure in dot-bracket notation:	...........((.........)).
The centroid secondary structure in dot-bracket notation:	...........((.........)).

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_231	Description	ICEL2.2
Aptamer chemistry	RNA	Length	25 nt
GC content	76.0%	Molecular weight	8,522.92 Da
Molarity of 1 μg/μl solution	117.33 μM	Number of G-quadruplexes	2
G-Score	17	Function	Targeted binding
Sequence	GGGAGGACGAUGCGGGUGUGGGGCG
Applications	IGEL1.2 and D17.4 competitively inhibit the interaction of human IgE with FceRI

Type	Detail	Type	Detail
Uniprot ID	P06734	Protein name	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Gene name(s)	FCER2 CD23A CLEC4J FCE2 IGEBF	Organism	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Status	reviewed in uniprot database	Length	321
Mass	36,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1T8C;1T8D;2H2R;2H2T;4EZM;4G96;4G9A;4GI0;4GJ0;4GJX;4GK1;4GKO;4J6J;4J6K;4J6L;4J6M;4J6N;4J6P;4J6Q;4KI1;5LGK;6Y0L;6Y0M;
Function	Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21.

	Interaction ID	236
	Target type	Protein
	Target unique ID	P06734
	Activity	35 nM
	Binding Conditions/Buffer	PBS containing 1 mM MgCl2 (pH 7.4)
	Assay	Representative binding curves of oligonucleotide ligands to IgE. The fraction of "P-labeled oligonucleotides bound to nitrocellulose filters is plotted as a function of total protein concentration, and the data points were fitted as described
	PubMed ID	8683119

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_230	RNA	GGGAGGACGAUGCGGGCGUGAAUGGUGUUGUGAGG	68.57%
Apta_10	DNA	GGGGGGGGTGGCTGGGCGGGGTGGG	60.00%
Apta_925	RNA	GCUCGGCCAGGUCGCGGGUGGAUGUGAGGG	60.00%
Apta_703	RNA	GGGCGGAUGAGACGCUUGGCGUGUGCUGUGGAGAGUCAUCCG	57.14%
Apta_1195	DNA	GGGGTTGGGGGGAGGCGGGGAATGGGGG	57.14%
Apta_9	DNA	TGAGGGGGCGGCGGGGCCGGGCAGG	56.00%
Apta_11	DNA	AGAGGGGGGGGTCGGGGTGGGTCGC	56.00%
Apta_21	DNA	TGTGGGGGAGGACGGGCGGGGATGC	56.00%
Apta_27	DNA	AGGGGGAGGGGTGGGGCCGGGCAGA	56.00%
Apta_806	DNA	GGTTGGATGTAAGGTTGGAGGGGGG	56.00%
Apta_1194	DNA	GGGAGAGGTGGGAGCGGGGCCAGGG	56.00%
Apta_218	RNA	AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU	55.88%
Apta_370	RNA	GGGAGGACGCGUCGCCGUAAUGGAUGUUUUGCUCCCUG	55.26%
Apta_1193	DNA	GGGTTGGAGGTGGAGGGGAGTTGAGG	53.85%
Apta_371	RNA	GGCAGACCAUACGGGAGAGAAACUUGCC	53.57%
Apta_679	DNA	CAGGTGGCAGATCGGGTGTGGGTGGCCTGG	53.33%
Apta_683	DNA	GCAGTACGATCGGGGGTGGGTGGATGTAGG	53.33%
Apta_926	RNA	UCGGCAGCGGUUAGAGAGGUGGUGUGCGGG	53.33%
Apta_927	RNA	UUACAAUGAGGUGGGACGCGGGUUGUGGUG	53.33%
Apta_1246	DNA	GGAGATGTATGAGCGGGTGTTTGTGTTTGC	53.33%

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