Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1098
Aptamer sequence: ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA
Target unique ID: Other-44
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_1013 | NB2 | Other | Activated protein C (APC) | 1.3 nM | 1X PBS | 32486960 |
Structure information of aptamer
| Aptamer Sequence: | ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA |
| The optimal secondary structure in dot-bracket notation: | .((((((......((((........))))..)))))).. |
| The centroid secondary structure in dot-bracket notation: | .((((((......((((........))))..)))))).. |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_1013 | Description | NB2 |
| Aptamer chemistry | DNA | Length | 39 nt |
| GC content | 69.2% | Molecular weight | 12,139.77 Da |
| Molarity of 1 μg/μl solution | 82.37 μM | Number of G-quadruplexes | 2 |
| G-Score | 19 | Function | Targeted therapy |
| Sequence | ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA | ||
| Applications | G-NB3 inhibits the inactivation of activated cofactors V and VIII with IC50 values of 11.6 and 13.1 nM | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Target ID | Other-44 | Name | Activated protein C (APC) |
Activity data
 |
Interaction ID | 1098 |
| Target type | Other | |
| Target unique ID | Other-44 | |
| Activity | 1.3 nM | |
| Binding Conditions/Buffer | 1X PBS |
|
| Assay | Influence of APC aptamers on the anticoagulant activity of endogenously generated APC in human plasma. Pooled normal citrated human plasma was spiked with rabbit thrombomodulin (2 U/mL) and increasing concentrations of APC aptamers. Subsequently, thrombin formation was measured using the CAT method |
|
| PubMed ID | 32486960 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1256 | DNA | GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACA | |
| Apta_15 | DNA | TGAGGGGGGGGGAGGGCGGGGCTGC | |
| Apta_28 | DNA | AGTGGGGGGGGTGGAGCCGGGTAGA | |
| Apta_488 | DNA | CGACCAGGGTGGGAGGGGGACGGGCTGGCGGCGCGCGGTAATG | |
| Apta_1042 | DNA | ATCACGATATGCCCGGCTCATCGGGGTTCAGTTGGGCGGTCACATGGAAA | |
| Apta_8 | DNA | TGTGGGGGTGGACGGGCCGGGTAGA | |
| Apta_9 | DNA | TGAGGGGGCGGCGGGGCCGGGCAGG | |
| Apta_19 | DNA | TGAGGGGGGGGGAGGTCGGGCCTGC | |
| Apta_20 | DNA | AGAGGGGGGGGTGGGGGTGGCCTGC | |
| Apta_24 | DNA | TGTGGGGATGGATGGGCCGGGCTGC | |
| Apta_29 | DNA | AGAGGGGGGGGACGGCCCGGGTAGA | |
| Apta_30 | DNA | AGAGGGGGGGGTGGAGGGGGCTAGA | |
| Apta_218 | RNA | AGCCGCGAGGGGAGGGAUAGGGUAGGGCGCGGCU | |
| Apta_320 | DNA | GCACTCTGTGGGGGTGGACGGGCCGGGT | |
| Apta_364 | DNA | TATGGTATGCTGTGTGGTATGGGGTGGCGTGCTCT | |
| Apta_648 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC | |
| Apta_259 | DNA | GCAAGTATGAGCGCAGGAGTTAGGTCCCGTGGCGATGGGT | |
| Apta_965 | DNA | CACGGCATGTGTGGCCGGATGCCTATATCGGTCGACTGCA | |
| Apta_1189 | DNA | GCGGGCGGGGGGAGGGCGGCCGTGGGCTGCGAGTGGGAGG | |
| Apta_79 | DNA | ATCTCGAGTCATAGGGGGCGCGAACATACGCGGTTGGTGTGGTTGGCTGACTCGT |
DEV TOOL
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