ProForma 2.0
validated simple linear
GVKFAKRFWRFAKKAFKRFEK
Back to Primary literature
Primary-literature peptidemedium evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001133
HAZ
Aliases: HAZ
Normalized sequence
GVKFAKRFWRFAKKAFKRFEK
21 amino acids
Release PepDiana-V1-20260620
36
Activity records
1
Source papers
35
Positive
1
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
2676.2151 Da
Molecular formula
C131H199N37O24
Theoretical pI
11.7637
Net charge at pH 7
7.7569
GRAVY
-0.8619
Instability index
32.0429
Aromaticity
0.2857
Topological polar surface area
1040.23 Ų
cLogP
-4.1925
Hydrogen-bond donors
39
Hydrogen-bond acceptors
32
Rotatable bonds
93
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{G.V.K.F.A.K.R.F.W.R.F.A.K.K.A.F.K.R.F.E.K}$$$$V2.0
Canonical SMILES
validated
CC(NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C(C)NC(=O)C(Cc1ccccc1)NC(=O)C(CCCNC(=N)N)NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C(Cc1ccccc1)NC(=O)C(CCCNC(=N)N)NC(=O)C(CCCCN)NC(=O)C(C)NC(=O)C(Cc1ccccc1)NC(=O)C(CCCCN)NC(=O)C(NC(=O)CN)C(C)C)C(=O)NC(Cc1ccccc1)C(=O)NC(CCCCN)C(=O)NC(CCCNC(=N)N)C(=O)NC(Cc1ccccc1)C(=O)NC(CCC(=O)O)C(=O)NC(CCCCN)C(=O)O
Isomeric SMILES
validated
CC(C)[C@H](NC(=O)CN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O
Paper-level observations
Experimental evidence grouped by activity
Antimicrobial 32 records
PMC9715947
32 observationsAntimicrobialAntibacterialPositive observation
Record detail Table 2: S. aureus ATCC-29215 MIC 15 µM.
- Measure
- MIC
- Value
- 15 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: S. aureus ATCC-29215 MBC 15 µM.
- Measure
- MBC
- Value
- 15 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: E. coli ATCC-25922 MIC 20 µM.
- Measure
- MIC
- Value
- 20 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: E. coli ATCC-25922 MBC 20 µM.
- Measure
- MBC
- Value
- 20 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: S. aureus ATCC-BAA-41 MIC 20 µM.
- Measure
- MIC
- Value
- 20 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: S. aureus ATCC-BAA-41 MBC 20 µM.
- Measure
- MBC
- Value
- 20 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: E. coli ATCC-BAA-2452 MIC 20 µM.
- Measure
- MIC
- Value
- 20 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: E. coli ATCC-BAA-2452 MBC 20 µM.
- Measure
- MBC
- Value
- 20 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: S. aureus (ATCC BAA41) MBEC 30 µM.
- Measure
- other
- Value
- 30 (MBEC)
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: S. aureus (ATCC 29215) MBEC 30 µM.
- Measure
- other
- Value
- 30 (MBEC)
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: E. coli (ATCC BAA-2452) MBEC 40 µM.
- Measure
- other
- Value
- 40 (MBEC)
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail Table 3: E. coli (ATCC 25922) MBEC 40 µM.
- Measure
- other
- Value
- 40 (MBEC)
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli 25922, HAZ MIC in combination with LVX = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli 25922, HAZ MIC in combination with CHL = 0.3125 µM.
- Measure
- MIC
- Value
- 0.3125 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli 25922, HAZ MIC in combination with RIF = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli 25922, HAZ MIC in combination with AMP = 5 µM.
- Measure
- MIC
- Value
- 5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli 25922, HAZ MIC in combination with DOX = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli BAA-2452, HAZ MIC in combination with LVX = 0.625 µM.
- Measure
- MIC
- Value
- 0.625 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli BAA-2452, HAZ MIC in combination with CHL = 0.375 µM.
- Measure
- MIC
- Value
- 0.375 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli BAA-2452, HAZ MIC in combination with RIF = 17.5 µM.
- Measure
- MIC
- Value
- 17.5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli BAA-2452, HAZ MIC in combination with AMP = 0.625 µM.
- Measure
- MIC
- Value
- 0.625 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: E. coli BAA-2452, HAZ MIC in combination with DOX = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus 29215, HAZ MIC in combination with LVX = 0.625 µM.
- Measure
- MIC
- Value
- 0.625 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus 29215, HAZ MIC in combination with CHL = 0.3125 µM.
- Measure
- MIC
- Value
- 0.3125 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus 29215, HAZ MIC in combination with RIF = 5.0 µM.
- Measure
- MIC
- Value
- 5.0 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus 29215, HAZ MIC in combination with AMP = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus 29215, HAZ MIC in combination with DOX = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus BAA-41, HAZ MIC in combination with LVX = 5 µM.
- Measure
- MIC
- Value
- 5 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus BAA-41, HAZ MIC in combination with CHL = 0.375 µM.
- Measure
- MIC
- Value
- 0.375 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus BAA-41, HAZ MIC in combination with RIF = 2.5 µM.
- Measure
- MIC
- Value
- 2.5 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus BAA-41, HAZ MIC in combination with AMP = 0.3125 µM.
- Measure
- MIC
- Value
- 0.3125 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 4: S. aureus BAA-41, HAZ MIC in combination with DOX = 1.25 µM.
- Measure
- MIC
- Value
- 1.25 µM
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
Anticancer 3 records
PMC9715947
3 observationsAnticancerCytotoxicPositive observation
Record detail Section 3.5: HAZ displayed potent antiproliferative effects on A549 cells, with an IC50 around 5.44 µM.
- Measure
- other
- Value
- 5.44 µM
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerCytotoxicPositive observation
Record detail Section 3.5: Even 1 µM was sufficient to inhibit cell proliferation by around 20 %.
- Measure
- cell_viability
- Value
- Even 1 µM was sufficient to inhibit cell proliferation by around 20 %
- Target
- Homo sapiens
- Assay
- Not reported by source
AnticancerCytotoxicNegative / threshold
Record detail Section 3.5: more than 95 % viability inhibition achieved by treatment with 10 µM.
- Measure
- cell_viability
- Value
- more than 95 % viability inhibition achieved by treatment with 10 µM
- Target
- Homo sapiens
- Assay
- Not reported by source
Toxicity 1 records
PMC9715947
1 observationsToxicityHemolyticPositive observation
Record detail Section 3.4: HAZ peptide displayed minimal hemolytic toxicity on RBCs even at concentrations higher than therapeutic ones. Hemolytic reaction was only detected at peptide concentrations beyond 20 µM, and a hemolysis percentage around 20 % and beyond was detected at concentrations higher than 120 µM.
- Measure
- hemolysis
- Value
- minimal hemolytic toxicity ... Hemolytic reaction was only detected at peptide concentrations beyond 20 µM, and a hemolysis percentage around 20 % and beyond was detected at concentrations higher than 120 µM
- Target
- Homo sapiens
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001133, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00