PEPDIA-PLP-000492

In the CSE-induced injury model (cell viability: 50.89 ± 2.68% vs. control), pretreatment with tuna peptides S1, S5, S6, and S7 significantly restored viability... (Figure 1B).

pretreatment with S1, S5, S6, and S7 significantly reduced ROS levels in a concentration-dependent manner. (Figure 2A,B)

In comparison to the model group, all tuna peptide treatment groups at a concentration of 200 μM demonstrated enhanced cellular antioxidant capacity, characterized by increased CAT and SOD activities, elevated GSH levels, and reduced MDA levels (p < 0.001). (Figure 3)

treatment with tuna peptides S1, S5, S6, and S7, resulted in a significant reduction in green fluorescence and a notable increase in red fluorescence (p < 0.001), suggesting that these peptides effectively restored MMP and inhibited CSE-induced apoptosis. (Figure 6)

pretreatment with 200 μM tuna peptides S1, S5, S6, and S7 significantly increased Nrf2 mRNA expression and decreased Keap1 mRNA expression in MLE-12 cells compared to the model group. (Figure 8)

At a concentration of 200 μM, the nuclear Nrf2 levels in the S1 and S6 peptide-treated groups showed significant higher compared to the model group (p < 0.05). (Figure 9)

At a concentration of 200 μM, the tuna peptide treatment groups (S1, S6, and S7) showed a significant reduction in these inflammatory markers compared to the model group. (Figure 4)

treatment with 200 µM tuna peptides S1, S5, S6, and S7 significantly reduced p65 mRNA expression and increased IκBα mRNA expression (p < 0.001). (Figure 11A,B)

At a concentration of 200 µM, the apoptosis rate in the peptide-treated groups was significantly lower than that in the model group (p < 0.001). (Figure 5)

pretreatment with 200 μM tuna peptides S1, S5, S6, and S7 significantly increased the Bcl-2/Bax mRNA expression ratio compared to the model group, with highly significant differences (p < 0.001). (Figure 11C)

To assess the safety of tuna peptides, cells were treated with 200 µM of peptide S1–8, which maintained viability above 90%, comparable to the blank control (100.00 ± 18.18%) and non-toxic relative to the positive control (134.31 ± 10.75%) (Figure 1B).

tuna peptides S1, S5, S6, and S7 significantly promote the migration of MLE-12 cells and accelerate wound closure in a concentration-dependent manner. (Figure 7)