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Paper-scoped peptide mentionSequence reported

OSTI-2369

PMC12838809::PEP000002

Source publication

Bifunctional Peptides Generated by Optimising the Antimicrobial Activity of a Novel Trypsin-Inhibitory Peptide from Odorrana schmackeri

Biomolecules · 2026

DOI 10.3390/biom16010148 PMC12838809 PMID 41594688

Sequence status: reported
Standardization: Sequence ready
Source location: unknown
Activity records: 42

Sequence evidence

Reported molecular identity

ALKRALKRCWTKSIPPRPCFNH

Table 1: OSTI-2369 sequence ALKRALKRCWTKSIPPRPCF-NH2

Resolved peptide links

PEPDIA-PLP-000138

Experimental observations

Records linked to this mention

OtherProtease InhibitorPositive observation

Table 2: OSTI-2369 Trypsin Ki = 0.044 μM

Mention PMC12838809::PEP000002PMC12838809table

Measure: Ki
Value: 0.044 µM
Target: trypsin
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli BAA 2340

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli BAA 2340

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli NCTC 13846

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli NCTC 13846

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 16 μM against P. aeruginosa ATCC CRM 9027

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 16 μM against P. aeruginosa ATCC CRM 9027

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 32 μM against K. pneumoniae ATCC CRM 43861

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 32 µM
Target: Klebsiella pneumoniae
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 32 μM against K. pneumoniae ATCC CRM 43861

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 32 µM
Target: Klebsiella pneumoniae
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 256 μM against A. baumannii BAA 747

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 256 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 256 μM against A. baumannii BAA 747

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 256 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Negative / threshold

Table 3: OSTI-2369 MIC >512 μM against S. aureus ATCC CRM 6538

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Negative / threshold

Table 3: OSTI-2369 MIC >512 μM against E. faecium NCTC 12697

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Enterococcus faecium
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Positive observation

Table 3: OSTI-2369 MIC 16 μM against MRSA NCTC 12493

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Methicillin-resistant Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Positive observation

Table 3: OSTI-2369 MBC 16 μM against MRSA NCTC 12493

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Methicillin-resistant Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 3: OSTI-2369 MIC >512 μM against C. albicans ATCC 10231

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Candida albicans
Assay: Not reported by source

Source publication

ToxicityHemolyticNegative / threshold

Section 3.8: OSTI-2369 produced little haemolysis, less than 10% even at the highest concentrations (1-512 μM) against horse erythrocytes

Mention PMC12838809::PEP000002PMC12838809text

Measure: hemolysis
Value: <10% at 1-512 μM
Target: horse
Assay: Not reported by source

Source publication

ToxicityCytotoxicNegative / threshold

Section 3.8: OSTI-2369 had minimal impact on HaCaT cell proliferation at 0.1-10 μM but reduced proliferation by more than 20% at 100 μM

Mention PMC12838809::PEP000002PMC12838809text

Measure: cell_viability
Value: minimal impact at 0.1-10 μM; >20% reduction at 100 μM
Target: HaCaT
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 in 1% DMSO (control)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 in 1% DMSO (control)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with FeCl3

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with FeCl3

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 128 μM against E. coli ATCC CRM 8739 with CaCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 128 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 128 μM against E. coli ATCC CRM 8739 with CaCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 128 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 4 μM against E. coli ATCC CRM 8739 with NaCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 4 μM against E. coli ATCC CRM 8739 with NaCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with KCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with KCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 16 μM against E. coli ATCC CRM 8739 with MgCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with MgCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 4 μM against E. coli ATCC CRM 8739 with NH4Cl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 4 μM against E. coli ATCC CRM 8739 with NH4Cl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 16 μM against E. coli ATCC CRM 8739 with Serum

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with Serum

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 (Contrast)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 (Contrast)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with 0.25 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with 0.25 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with 0.5 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with 0.5 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.