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Primary-literature peptidehigh evidence

PEPDIA-PLP-000138

OSTI-2369

Aliases: OSTI-2369

Normalized sequence

ALKRALKRCWTKSIPPRPCFNH

22 amino acids

Release PepDiana-V1-20260620

42

Activity records

1

Source papers

37

Positive

5

Negative / threshold

Versioned scientific descriptors

Physicochemical properties and standard representations

calculated sequence and structure

Reported properties

No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.

Computed properties

physchem-20260620

Molecular weight

2623.1561 Da

Molecular formula

C118H192N38O26S2

Theoretical pI

11.017

Net charge at pH 7

5.8602

GRAVY

-0.7091

Instability index

44.7409

Aromaticity

0.0909

Topological polar surface area

1039.83 Å²

cLogP

-8.5068

Hydrogen-bond donors

39

Hydrogen-bond acceptors

35

Rotatable bonds

82

Method: PepDiana sequence/structure descriptor pipeline

Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6

Calculated: 2026-06-20T11:01:38.358971+00:00

Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200

Sequence representations

representations-20260620

ProForma 2.0

validated simple linear

ALKRALKRCWTKSIPPRPCFNH

HELM 2.0

validated simple linear

PEPTIDE1{A.L.K.R.A.L.K.R.C.W.T.K.S.I.P.P.R.P.C.F.N.H}$$$$V2.0

Canonical SMILES

validated

CCC(C)C(NC(=O)C(CO)NC(=O)C(CCCCN)NC(=O)C(NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C(CS)NC(=O)C(CCCNC(=N)N)NC(=O)C(CCCCN)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCCNC(=N)N)NC(=O)C(CCCCN)NC(=O)C(CC(C)C)NC(=O)C(C)N)C(C)O)C(=O)N1CCCC1C(=O)N1CCCC1C(=O)NC(CCCNC(=N)N)C(=O)N1CCCC1C(=O)NC(CS)C(=O)NC(Cc1ccccc1)C(=O)NC(CC(N)=O)C(=O)NC(Cc1c[nH]cn1)C(=O)O

Isomeric SMILES

validated

CC[C@H](C)[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CS)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)N)[C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O

Paper-level observations

Experimental evidence grouped by activity

Antimicrobial 39 records

PMC12838809

39 observations

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli BAA 2340

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli BAA 2340

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 8 μM against E. coli NCTC 13846

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 8 μM against E. coli NCTC 13846

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 16 μM against P. aeruginosa ATCC CRM 9027

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 16 μM against P. aeruginosa ATCC CRM 9027

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 32 μM against K. pneumoniae ATCC CRM 43861

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 32 µM
Target: Klebsiella pneumoniae
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 32 μM against K. pneumoniae ATCC CRM 43861

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 32 µM
Target: Klebsiella pneumoniae
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MIC 256 μM against A. baumannii BAA 747

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 256 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 3: OSTI-2369 MBC 256 μM against A. baumannii BAA 747

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 256 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Negative / threshold

Table 3: OSTI-2369 MIC >512 μM against S. aureus ATCC CRM 6538

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Negative / threshold

Table 3: OSTI-2369 MIC >512 μM against E. faecium NCTC 12697

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Enterococcus faecium
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Positive observation

Table 3: OSTI-2369 MIC 16 μM against MRSA NCTC 12493

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Methicillin-resistant Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram+Positive observation

Table 3: OSTI-2369 MBC 16 μM against MRSA NCTC 12493

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Methicillin-resistant Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 3: OSTI-2369 MIC >512 μM against C. albicans ATCC 10231

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: >512 µM
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 in 1% DMSO (control)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 in 1% DMSO (control)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with FeCl3

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with FeCl3

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 128 μM against E. coli ATCC CRM 8739 with CaCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 128 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 128 μM against E. coli ATCC CRM 8739 with CaCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 128 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 4 μM against E. coli ATCC CRM 8739 with NaCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 4 μM against E. coli ATCC CRM 8739 with NaCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with KCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with KCl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 16 μM against E. coli ATCC CRM 8739 with MgCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with MgCl2

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 4 μM against E. coli ATCC CRM 8739 with NH4Cl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 4 μM against E. coli ATCC CRM 8739 with NH4Cl

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 4 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MIC 16 μM against E. coli ATCC CRM 8739 with Serum

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 4: OSTI-2369 MBC 16 μM against E. coli ATCC CRM 8739 with Serum

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 16 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 (Contrast)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 (Contrast)

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with 0.25 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with 0.25 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MIC 8 μM against E. coli ATCC CRM 8739 with 0.5 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MIC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAnti-Gram-Positive observation

Table 5: OSTI-2369 MBC 8 μM against E. coli ATCC CRM 8739 with 0.5 mg/mL trypsin

Mention PMC12838809::PEP000002PMC12838809table

Measure: MBC
Value: 8 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

Toxicity 2 records

PMC12838809

2 observations

ToxicityHemolyticNegative / threshold

Section 3.8: OSTI-2369 produced little haemolysis, less than 10% even at the highest concentrations (1-512 μM) against horse erythrocytes

Mention PMC12838809::PEP000002PMC12838809text

Measure: hemolysis
Value: <10% at 1-512 μM
Target: horse
Assay: Not reported by source

Source publication

ToxicityCytotoxicNegative / threshold

Section 3.8: OSTI-2369 had minimal impact on HaCaT cell proliferation at 0.1-10 μM but reduced proliferation by more than 20% at 100 μM

Mention PMC12838809::PEP000002PMC12838809text

Measure: cell_viability
Value: minimal impact at 0.1-10 μM; >20% reduction at 100 μM
Target: HaCaT
Assay: Not reported by source

Source publication

Other 1 records

PMC12838809

1 observations

OtherProtease InhibitorPositive observation

Table 2: OSTI-2369 Trypsin Ki = 0.044 μM

Mention PMC12838809::PEP000002PMC12838809table

Measure: Ki
Value: 0.044 µM
Target: trypsin
Assay: Not reported by source

Source publication

Citation and release provenance

Cite this identity as PepDiana record PEPDIA-PLP-000138, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.

Record updated: 2026-06-22T07:14:26.229247+00:00