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Paper-scoped peptide mentionSequence reported

MR-22

PMC10847306::PEP000001

Source publication

The antibacterial activity and mechanism of a novel peptide MR-22 against multidrug-resistant Escherichia coli.

Frontiers in Cellular and Infection Microbiology · 2024

DOI 10.3389/fcimb.2024.1334378 PMC10847306 PMID 38328670

Sequence status: reported
Standardization: Sequence ready
Source location: unknown
Activity records: 71

Sequence evidence

Reported molecular identity

MAKRRKKAKKKAKKAKKRRRRRNH

Table 1: MR-22 sequence MAKRRKKAKKKAKKAKKRRRRR-NH2, MW 2808.58, charge +17, purity 95.44%.

Resolved peptide links

PEPDIA-PLP-001966

Experimental observations

Records linked to this mention

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli ATCC 25922 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli ATCC 25922 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E01 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E01 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E02 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E02 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E03 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E03 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E04 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E04 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E05 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E05 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E06 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E06 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E07 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E07 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E08 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E08 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E09 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E09 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E10 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E10 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E11 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E11 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E12 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E12 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E13 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E13 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E14 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E14 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E15 is 4 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 4 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E15 is 4 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 4 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E16 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E16 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E17 is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E17 is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E18 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E18 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E19 is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E19 is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E20 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E20 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E21 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E21 is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MIC against E. coli E22 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: MR-22 MBC against E. coli E22 is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MBC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 under Control condition is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 under Control condition is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 150 μM Na+ is 256 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 256 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 in presence of 150 μM Na+ is 256 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 256 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 2 mM Ca2+ is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 in presence of 2 mM Ca2+ is 8 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 8 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 10% FBS is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 in presence of 10% FBS is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 10% Serum is 64 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 64 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 in presence of 10% Serum is 128 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 128 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 3: MR-22 MIC against E. coli ATCC 25922 after trypsin treatment is >256 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: >256 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 3: MR-22 MIC against E. coli E19 after trypsin treatment is >256 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: >256 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 after 40°C 30 min is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 after 40°C 30 min is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 after 60°C 30 min is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 after 60°C 30 min is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 after 80°C 30 min is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 after 80°C 30 min is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli ATCC 25922 after 100°C 30 min is 16 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 16 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: MR-22 MIC against E. coli E19 after 100°C 30 min is 32 μg/mL.

Mention PMC10847306::PEP000001PMC10847306table

Measure: MIC
Value: 32 µg/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

ToxicityCytotoxicPositive observation

Section 3.5: cell viability of HK-2 cells was lowest after treatment with 256 μg/mL of MR-22, with a value of 75.60%.

Mention PMC10847306::PEP000001PMC10847306text

Measure: cell_viability
Value: 75.60%
Target: HK-2
Assay: Not reported by source

Source publication

ToxicityCytotoxicNegative / threshold

Section 3.5: cell viability of L-O2 cells treated with various concentrations of MR-22 was consistently above 100%.

Mention PMC10847306::PEP000001PMC10847306text

Measure: cell_viability
Value: above 100%
Target: L-O2
Assay: Not reported by source

Source publication

ToxicityHemolyticPositive observation

Section 3.5: MR-22 displayed low hemolytic activities against human erythrocytes (Figure 2F).

Mention PMC10847306::PEP000001PMC10847306text

Measure: hemolysis
Value: low hemolytic activities
Target: Homo sapiens
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Section 3.9: In C57BL/6J mice model, the 72-h survival rate of the MR-22 group was 40%.

Mention PMC10847306::PEP000001PMC10847306text

Measure: survival_rate
Value: 40%
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Section 3.9: bacterial content in peritoneal lavage fluid of peritonitis mice treated with MR-22 decreased to 7.23 ± 0.36 Log10CFU/mL.

Mention PMC10847306::PEP000001PMC10847306text

Measure: other
Value: 7.23 ± 0.36 Log10CFU/mL
Target: Escherichia coli
Assay: Not reported by source

Source publication

Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.