ProForma 2.0
validated simple linear
MAKRRKKAKKKAKKAKKRRRRRNH
Back to Primary literature
Primary-literature peptidehigh evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001966
MR-22
Aliases: MR-22
Normalized sequence
MAKRRKKAKKKAKKAKKRRRRRNH
24 amino acids
Release PepDiana-V1-20260620
71
Activity records
1
Source papers
68
Positive
3
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
3059.7877 Da
Molecular formula
C129H248N58O26S
Theoretical pI
12
Net charge at pH 7
16.5775
GRAVY
-2.8375
Instability index
166.3667
Aromaticity
0
Topological polar surface area
1497.89 Ų
cLogP
-16.6445
Hydrogen-bond donors
58
Hydrogen-bond acceptors
45
Rotatable bonds
122
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{M.A.K.R.R.K.K.A.K.K.K.A.K.K.A.K.K.R.R.R.R.R.N.H}$$$$V2.0
Canonical SMILES
validated
CSCCC(N)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC(N)=O)C(=O)NC(Cc1c[nH]cn1)C(=O)O
Isomeric SMILES
validated
CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O
Paper-level observations
Experimental evidence grouped by activity
Antimicrobial 68 records
PMC10847306
68 observationsAntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli ATCC 25922 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli ATCC 25922 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E01 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E01 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E02 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E02 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E03 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E03 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E04 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E04 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E05 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E05 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E06 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E06 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E07 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E07 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E08 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E08 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E09 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E09 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E10 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E10 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E11 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E11 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E12 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E12 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E13 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E13 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E14 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E14 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E15 is 4 μg/mL.
- Measure
- MIC
- Value
- 4 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E15 is 4 μg/mL.
- Measure
- MBC
- Value
- 4 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E16 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E16 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E17 is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E17 is 32 μg/mL.
- Measure
- MBC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E18 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E18 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E19 is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E19 is 32 μg/mL.
- Measure
- MBC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E20 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E20 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E21 is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E21 is 8 μg/mL.
- Measure
- MBC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MIC against E. coli E22 is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 2: MR-22 MBC against E. coli E22 is 16 μg/mL.
- Measure
- MBC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 under Control condition is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 under Control condition is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 150 μM Na+ is 256 μg/mL.
- Measure
- MIC
- Value
- 256 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 in presence of 150 μM Na+ is 256 μg/mL.
- Measure
- MIC
- Value
- 256 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 2 mM Ca2+ is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 in presence of 2 mM Ca2+ is 8 μg/mL.
- Measure
- MIC
- Value
- 8 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 10% FBS is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 in presence of 10% FBS is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 in presence of 10% Serum is 64 μg/mL.
- Measure
- MIC
- Value
- 64 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 in presence of 10% Serum is 128 μg/mL.
- Measure
- MIC
- Value
- 128 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 after trypsin treatment is >256 μg/mL.
- Measure
- MIC
- Value
- >256 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail Table 3: MR-22 MIC against E. coli E19 after trypsin treatment is >256 μg/mL.
- Measure
- MIC
- Value
- >256 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 after 40°C 30 min is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 after 40°C 30 min is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 after 60°C 30 min is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 after 60°C 30 min is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 after 80°C 30 min is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 after 80°C 30 min is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli ATCC 25922 after 100°C 30 min is 16 μg/mL.
- Measure
- MIC
- Value
- 16 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Table 3: MR-22 MIC against E. coli E19 after 100°C 30 min is 32 μg/mL.
- Measure
- MIC
- Value
- 32 µg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Section 3.9: In C57BL/6J mice model, the 72-h survival rate of the MR-22 group was 40%.
- Measure
- survival_rate
- Value
- 40%
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail Section 3.9: bacterial content in peritoneal lavage fluid of peritonitis mice treated with MR-22 decreased to 7.23 ± 0.36 Log10CFU/mL.
- Measure
- other
- Value
- 7.23 ± 0.36 Log10CFU/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
Toxicity 3 records
PMC10847306
3 observationsToxicityCytotoxicPositive observation
Record detail Section 3.5: cell viability of HK-2 cells was lowest after treatment with 256 μg/mL of MR-22, with a value of 75.60%.
- Measure
- cell_viability
- Value
- 75.60%
- Target
- HK-2
- Assay
- Not reported by source
ToxicityCytotoxicNegative / threshold
Record detail Section 3.5: cell viability of L-O2 cells treated with various concentrations of MR-22 was consistently above 100%.
- Measure
- cell_viability
- Value
- above 100%
- Target
- L-O2
- Assay
- Not reported by source
ToxicityHemolyticPositive observation
Record detail Section 3.5: MR-22 displayed low hemolytic activities against human erythrocytes (Figure 2F).
- Measure
- hemolysis
- Value
- low hemolytic activities
- Target
- Homo sapiens
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001966, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00