Communications Biology · 2023
Sequence evidence
FLPLIGRVLSGIL
FLPLIGRVLSGIL
Experimental observations
the range of antimicrobial activity of the hybrid peptide matched, or came within one dilution, of that of the template AMP (protonecin and temporin A, 3.1–25 μmol L−1).
the parent peptides decoralin and temporin A showed no activity.
Within 4 h of incubation with MCF-7, protonectin and temporin A reduced the counts of viable MCF-7 cells (LD50: 25 μmol L−1 and 50 μmol L−1, respectively).
After 24 h of incubation, protonectin and temporin A exhibited the same LD50 (LD50: 25 μmol L−1 and 50 μmol L−1, respectively).
Hemolytic activity, here defined as the concentration of peptide needed to kill 10% of red blood cells, was observed with the parent peptides decoralin and temporin, which were hemolytic at 1.6 μmol L−1 and 0.2 μmol L−1, respectively.
VT presented the same effect as the two parent peptides.
After 24 h of incubation, we did not observe peptide-meditated cytotoxicity towards MCF-10A cells (Fig. 3e).
Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.
