Communications Biology · 2023
Sequence evidence
SLLSLIRKLIT
SLLSLIRKLIT
Experimental observations
The template peptides (VmCT1, anoplin, protonectin, decoralin, and temporin A) exhibited MICs against bacterial strains ranging from 0.8 to 25 μmol L−1 (Fig. 3a and Tables S1 and S2).
the parent peptides decoralin and temporin A showed no activity.
VD showed decreased activity compared to its template peptides VmCT1 and decoralin (50 μmol L−1).
Hemolytic activity, here defined as the concentration of peptide needed to kill 10% of red blood cells, was observed with the parent peptides decoralin and temporin, which were hemolytic at 1.6 μmol L−1 and 0.2 μmol L−1, respectively.
After 24 h of incubation, we did not observe peptide-meditated cytotoxicity towards MCF-10A cells (Fig. 3e).
Local peptide labels such as P1, PEP1, or peptide 1 are grouped only within this paper and are not assumed to represent the same molecule across publications.
