AptaDB: Result

Interaction ID: 234

Aptamer sequence: GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC

Target unique ID: P06734

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_229	D17.4	Protein	FCER2_HUMAN	10 nM	PBS containing 1 mM MgCl2 (pH 7.4)	8683119

Aptamer Sequence:	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC
The optimal secondary structure in dot-bracket notation:	((((((((((.................))))))))))
The centroid secondary structure in dot-bracket notation:	((((((((((.................))))))))))

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_229	Description	D17.4
Aptamer chemistry	DNA	Length	37 nt
GC content	67.6%	Molecular weight	11,525.15 Da
Molarity of 1 μg/μl solution	86.77 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC
Applications	IGEL1.2 and D17.4 competitively inhibit the interaction of human IgE with FceRI

Type	Detail	Type	Detail
Uniprot ID	P06734	Protein name	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Gene name(s)	FCER2 CD23A CLEC4J FCE2 IGEBF	Organism	Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) (C-type lectin domain family 4 member J) (Fc-epsilon-RII) (Immunoglobulin E-binding factor) (Lymphocyte IgE receptor) (CD antigen CD23) [Cleaved into: Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form]
Status	reviewed in uniprot database	Length	321
Mass	36,469	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	1T8C;1T8D;2H2R;2H2T;4EZM;4G96;4G9A;4GI0;4GJ0;4GJX;4GK1;4GKO;4J6J;4J6K;4J6L;4J6M;4J6N;4J6P;4J6Q;4KI1;5LGK;6Y0L;6Y0M;
Function	Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21.

	Interaction ID	234
	Target type	Protein
	Target unique ID	P06734
	Activity	10 nM
	Binding Conditions/Buffer	PBS containing 1 mM MgCl2 (pH 7.4)
	Assay	Representative binding curves of oligonucleotide ligands to IgE. The fraction of "P-labeled oligonucleotides bound to nitrocellulose filters is plotted as a function of total protein concentration, and the data points were fitted as described
	PubMed ID	8683119

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_543	DNA	GGCACGTGTTGTCTCTCTGTGTCTCGTGCC	62.16%
Apta_1027	DNA	GGAGCTACGTTTAGCGAGTCCGACGCTCGGTGCCTCTTC	61.54%
Apta_73	DNA	GGGGAGTAGGTTGGTGTGGTTGGGGCTCCCC	56.76%
Apta_338	DNA	GGGCGCGTTCTTCGTGGTTACTTTTAGTCCCG	56.76%
Apta_542	DNA	GCACGTGTTGTCTCTCTGTGTCTCGTGC	56.76%
Apta_914	DNA	CGCTCCGTTTACTCGGTCTGCTATTGGCTTGCCTTTGTTT	55.00%
Apta_1263	DNA	CCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCG	55.00%
Apta_146	DNA	GGTGCGGTTCGTGCGGTTGTAGTACTCGTGGCCG	54.05%
Apta_360	DNA	GGGAACGCATACGCCGCAGTATTTCCC	54.05%
Apta_541	DNA	CACGTGTTGTCTCTCTGTGTCTCGTG	54.05%
Apta_1147	DNA	GGGGACTAGAGGACTTGTGCGGCC	54.05%
Apta_602	DNA	GGGCGAGAACAGTGTCCCAGTCGCTAGTTTTCTCTGCCC	53.85%
Apta_586	DNA	GGGAGACAAGGAAAATCCTTCAATGAAGTGGGTCGACA	52.63%
Apta_160	DNA	CGGTGCAGTGGATACATGCCAGCCGTAGCCATCGTGGATA	52.50%
Apta_556	DNA	GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC	52.08%
Apta_356	DNA	CGGGGGGTACGTGCCCGTGTTGCTCGGCGGCCCCCTCGCTTATTTTTGTGTGCCCTTT	51.72%
Apta_981	DNA	TATGGAGAGTCAATGAGTACAGTCTCGTCTCAATAACGGAGCCTCGTCCGTGCCCCCG	51.72%
Apta_71	DNA	GGCGAGTAGGTTGGTGTGGTTGGGGCTCGCC	51.35%
Apta_74	DNA	GGACAGTAGGTTGGTGTGGTTGGGGCTGTCC	51.35%
Apta_214	DNA	AGGAAGGCTTTAGGTTTCCTAAAGCCTTCC	51.35%

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