AptaDB: Result

Interaction ID: 1178

Aptamer sequence: ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA

Target unique ID: P0DPI0

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_1122	S132B-C12	Protein	BXA1_CLOBO	111 nM	PBS pH 7.4, 300 mM NaCl, 3 mM MgCl2, 5 mM DTT 1 mg/ml BSA, 0.2% Tween, 1 mg/ml heparin	20452328

Aptamer Sequence:	ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA
The optimal secondary structure in dot-bracket notation:	.....((((..........................))))......
The centroid secondary structure in dot-bracket notation:	.....((((..........................))))......

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_1122	Description	S132B-C12
Aptamer chemistry	RNA	Length	45 nt
GC content	48.9%	Molecular weight	14,342.65 Da
Molarity of 1 μg/μl solution	69.72 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted therapy
Sequence	ACAACCCGGAACAACGUCUAACAGUGUACCAUAACCCGGCAUUCA
Applications	Reverse the symptom caused by BoNT/A

Type	Detail	Type	Detail
Uniprot ID	P0DPI0	Protein name	Botulinum neurotoxin type A (BoNT/A) (Bontoxilysin-A) (BOTOX) (Botulinum neurotoxin type A1) [Cleaved into: Botulinum neurotoxin A light chain (LC) (EC 3.4.24.69); Botulinum neurotoxin A heavy chain (HC)]
Gene name(s)	botA atx bonT	Organism	Botulinum neurotoxin type A (BoNT/A) (Bontoxilysin-A) (BOTOX) (Botulinum neurotoxin type A1) [Cleaved into: Botulinum neurotoxin A light chain (LC) (EC 3.4.24.69); Botulinum neurotoxin A heavy chain (HC)]
Status	reviewed in uniprot database	Length	1296
Mass	149,454	Activity regulation	Toxin internalization is inhibited by azide or dinitrophenol or at 4 degrees Celsius
Involvement in disease	N/A	Catalytic activity	Reaction=Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin
PDB ID(s)	1XTF;1XTG;2ILP;2IMA;2IMB;2IMC;2ISE;2ISG;2ISH;2VU9;2VUA;2W2D;3BOK;3BON;3BOO;3V0A;3V0B;3V0C;3ZUR;3ZUS;4HEV;4IQP;4JRA;5JLV;5JMC;5MK6;5MK7;5TPB;5TPC;5V8P;5V8R;5V8U;5VGV;5VGX;6DKK;6MHJ;6XCB;6XCC;6XCD;6XCE;6XCF;7KYF;7KYH;7LZP;7M1H;
Function	Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure.

	Interaction ID	1178
	Target type	Protein
	Target unique ID	P0DPI0
	Activity	111 nM
	Binding Conditions/Buffer	PBS pH 7.4, 300 mM NaCl, 3 mM MgCl2, 5 mM DTT 1 mg/ml BSA, 0.2% Tween, 1 mg/ml heparin
	Assay	Binding specificity and affinity of aptmers
	PubMed ID	20452328

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_1	RNA	ACGAAGCUUGAUCCCGUUUGCCGGUCGAUCGCUUCGA	53.33%
Apta_85	RNA	UGCGACUGUUAUAACCUAACAGCGACGUAAAGAUA	53.33%
Apta_1211	RNA	ACUAGCCUCAUCAGCUCAUGUGCCCCUCCGCCUGGAUCAC	53.33%
Apta_447	RNA	ACGCUCAACGAGCCAGGAACAUCGACGUCAGCAAACGCGAGCGCAACCAGUAACACC	52.63%
Apta_90	RNA	ACAACCUAAAUGCGACGUUGGGUCAAAAACGUGAA	51.11%
Apta_262	DNA	ACGGACTCGCAAAAGGTGGAACAGGAGTGGGCCCCGCGGC	51.11%
Apta_633	RNA	GGCAUCGGAAAGUGGGUUGAUGUAAGUAACAGGCGAUGCC	51.11%
Apta_1123	RNA	AUUCGGGCCCAGGAACCAACUAUAUAAAUGUCCCGAAUGCUUCGACG	51.06%
Apta_301	RNA	AUACCAGCUUAUUCAAUUGCCUGAAAAGUUGAACUCCAAAUACGCGCUGAGAUAGUAAGUGCAAUCU	49.25%
Apta_520	RNA	GGGAGAUCUACGGAUCUCAGGGCUCUUACGGGAGCUACAUGGAAGGAGUCCAUGUGU	49.12%
Apta_154	RNA	ACACGCGAGAUAGAGCGAGGCCUCCAAAAAUGGCCACGCCAGGAAGCAAGU	49.02%
Apta_1121	RNA	GACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGC	48.94%
Apta_93	RNA	CUCCGCUAUCUAACGUAUGAUAGAAUGGAUGACUA	48.89%
Apta_94	RNA	AUGACAGGAUGCAGAGCUCCACUGUCUAGUGUUUA	48.89%
Apta_142	DNA	CGCACATCAGTTTAGCAGCGCCAAAGGCGGAAGCGCA	48.89%
Apta_242	RNA	GCUUCCAGGAAGAGAAGUGUCAUGUGAUCAGUCCUGGUGGGUGG	48.89%
Apta_313	RNA	UGCCCUGCCCUCACCCGUUAGCCUGAGCGCCCCGCA	48.89%
Apta_385	DNA	ACAGATACGGAACTACAGAGGTCAATTACGGTGGCCACGC	48.89%
Apta_513	DNA	TAGGGAAAACCAACCGCCCAGAACTGCAGCATAAC	48.89%
Apta_647	RNA	GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC	48.89%

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