Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 889
Aptamer sequence: ATCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGA
Target unique ID: CRL-8199
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_699 | sgc8 | Cell | leukemia cells | 0.78 nM | 0.1 mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl2 in Dulbecco's PBS with CaCl2 and MgCl2) | 17373017 |
Structure information of aptamer
| Aptamer Sequence: | ATCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGA |
| The optimal secondary structure in dot-bracket notation: | .((((((((.((((((....)).......)))))))))))) |
| The centroid secondary structure in dot-bracket notation: | .((((((((.((((((....)).......)))))))))))) |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_699 | Description | sgc8 |
| Aptamer chemistry | DNA | Length | 41 nt |
| GC content | 51.2% | Molecular weight | 12,634.13 Da |
| Molarity of 1 μg/μl solution | 79.15 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Targeted therapy |
| Sequence | ATCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGA | ||
| Applications | Fluorescence signal for tumor cell imaging and diagnosis; APCs efficiently penetrated into the interior of multicellular tumor spheroids (MCTS) and caused cell damage | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | CRL-8199 | Cell name | leukemia cells |
| Category | Animal cells | Organism | N/A |
| Product type | N/A | Cell type | N/A |
| Morphology | lymphoblast | Tissue | N/A |
| Disease | N/A | Product format | Frozen |
| Antigen expression | N/A | Genes expressed | N/A |
| Cell description | N/A |
|
|
| Application(s) | Immune system disorder research; Immunology | ||
Activity data
|
No relevant experimental diagram |
Interaction ID | 771 |
| Target type | Molecule | |
| Target unique ID | 161456 | |
| Activity | N/A | |
| Binding Conditions/Buffer | N/A |
|
| Assay | N/A |
|
| PubMed ID | 32584586 | |
|
No relevant experimental diagram |
Interaction ID | 889 |
| Target type | Cell | |
| Target unique ID | CRL-8199 | |
| Activity | 0.78 nM | |
| Binding Conditions/Buffer | 0.1 mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl2 in Dulbecco's PBS with CaCl2 and MgCl2) |
|
| Assay | N/A |
|
| PubMed ID | 17373017 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_1060 | DNA | AGCAATATTGCCCGCGCGGGGCCAACTGTCCTCACATGA | |
| Apta_1087 | DNA | CTTACGACTCAGGCATTTTGCCGGGTAACGAAGTTACTGTCGTAAG | |
| Apta_808 | DNA | GGGTCTTCCTGGACTGTCGAAAATTCAGTATCGGGAGG | |
| Apta_1149 | DNA | ATCATGGTGGGTATCGGCACTCGTTGGTTGAT | |
| Apta_750 | DNA | ATGCGAACAGGTGGGTGGGTTGGGTGGATTGTTCGGCTTCTTGAT | |
| Apta_1034 | DNA | ATGCGGATCCCGCGCGCGACCGTGTCAGCGGGGACTAGCGGTGTAGCGCGAAGCTTGCGC | |
| Apta_1230 | DNA | GGCGATAGGCAGTGTTGCGGGGTCGGAGAGCGAGGTAATAGCGTGTATGGGTGCTGTGTG | |
| Apta_65 | DNA | CTACCGTGGTAGGGAAGGTTGGAGTGTAG | |
| Apta_829 | DNA | CCCTCCGGGGGGGTCATCGGGATACCTGGTAAGGATA | |
| Apta_1013 | DNA | ATCACGTATGGGGGGCCGGCATGAGGGCCGCGCGTGACA | |
| Apta_1208 | DNA | CCCTGCGGGGCTGCCCGATATGTGTCCAGGTGGTG | |
| Apta_1262 | DNA | ACAAGGGGTGCAGGGGTCCGCGATTGTGACTGTGTGTGCG | |
| Apta_641 | DNA | TTCAAGACGATGCCTGGCGCGAGTTACACACTTGCATGGAGCTGG | |
| Apta_282 | DNA | ATCCATGGGGCGGAGATGAGGGGGAGGAGGGCGGGTACCCGGTTGAT | |
| Apta_421 | DNA | ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA | |
| Apta_621 | DNA | AAGCTTGCTTTATAGCCTGCAGCGATTCTTGATCGGAAAAGGCTGAGAGCTACGC | |
| Apta_279 | DNA | TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGAGATGTTTGGTTTT | |
| Apta_523 | DNA | ATCCGTCACACCTGCTCTGACGCTGGGGTCGACCCGGAGAAATGCATTCCCCTGTGGTGTTGGCTCCCGTAT | |
| Apta_664 | DNA | CACGCAACAACACAACATGCCCAGCGCCTGGAACATATCCTATGAGTTAGTCCGCCCACA | |
| Apta_591 | DNA | CTCTCGGGACGACGGCTGGCACGTTTGGTTCAAGAATGTGGGTGTCGTCCC |
DEV TOOL
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