Aptamer-target interaction descriptor
Aptamer-target interaction information
| Aptamer ID | Aptamer descriptor | Target chemistry | Target name | Affinity | Binding Conditions/Buffer | PubMed ID |
|---|---|---|---|---|---|---|
| Apta_781 | JH6 | Cell | NB4 AML cell line | 2.77 nM | 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) | 24405684 |
Structure information of aptamer
| Aptamer Sequence: | GTACGCCGCAAGACGAGTTGTGTATAAGCCGGC |
| The optimal secondary structure in dot-bracket notation: | ((((((.((.......)).))))))........ |
| The centroid secondary structure in dot-bracket notation: | ((((((.((.......)).))))))........ |
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
| Type | Detail | Type | Detail |
|---|---|---|---|
| Aptamer ID | Apta_781 | Description | JH6 |
| Aptamer chemistry | DNA | Length | 33 nt |
| GC content | 57.6% | Molecular weight | 10,203.57 Da |
| Molarity of 1 μg/μl solution | 98.00 μM | Number of G-quadruplexes | No QGRS found |
| G-Score | N/A | Function | Aptasensor |
| Sequence | GTACGCCGCAAGACGAGTTGTGTATAAGCCGGC | ||
| Applications | Probes for acute myelogenous leukemia detection and surface protein biomarker discovery | ||
Target information
| Type | Detail | Type | Detail |
|---|---|---|---|
| ATCC ID | TIB-202 | Cell name | NB4 AML cell line |
| Category | Human cells | Organism | Homo sapiens, human |
| Product type | N/A | Cell type | monocyte |
| Morphology | monocyte | Tissue | Peripheral blood |
| Disease | Acute monocytic leukemia | Product format | Frozen |
| Antigen expression | HLA A2, A9, B5, DRw1, DRw2 | Genes expressed | Lysozyme; HLA: (A2; A9; B5; DRw2) |
| Cell description | THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. |
|
|
| Application(s) | 3D cell culture; Immune system disorder research; Immunology; Toxicology | ||
Activity data
 |
Interaction ID | 865 |
| Target type | Cell | |
| Target unique ID | TIB-202 | |
| Activity | 2.77 nM | |
| Binding Conditions/Buffer | 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) |
|
| Assay | Determination of the aptamer binding affinities to NB4 cells |
|
| PubMed ID | 24405684 |
Similar aptamers
| Aptamer ID | Aptamer chemistry | Sequence | Similarity |
|---|---|---|---|
| Apta_58 | DNA | GCACCTTGATGACATGATAGTCGTTGTGTATGCAGTTGGC | |
| Apta_544 | DNA | GCGGGCGGTTGTATAGCGG | |
| Apta_987 | DNA | GTACTGTCAATTGGAAGTGGTGTTACGTTGTGTAGTCAAATCAGTGC | |
| Apta_1206 | DNA | GGCAGCAGCAATGTAACACTGTGTGTATGTGTTGG | |
| Apta_648 | DNA | GCACACCGATGGCGGTCCTGTTTAGGGGGTGTGC | |
| Apta_506 | DNA | AAGCGCGCGAGACTACGTTTGTAGGGATACCGATGTCG | |
| Apta_967 | DNA | GGGTAGCAGCCCCGCGGAGGGTCGGCTATAAGAACCAAGA | |
| Apta_519 | DNA | GTATTGCGGAGGAAGGTTTTTAACCTTCGGGG | |
| Apta_673 | DNA | GCAGTCCTAGATCGGGTGTGGCTGGCTTGG | |
| Apta_678 | DNA | CGTACGGTGGGAACGGTTCCTCTTAGGGT | |
| Apta_679 | DNA | CAGGTGGCAGATCGGGTGTGGGTGGCCTGG | |
| Apta_77 | DNA | GGTGTGGGCGACATGGGGGTTGGTGGTGTAGGAGCGCCAT | |
| Apta_285 | DNA | GCCACTCCGTCACTTTAGTGGTTTTTTTTATAAATGCTTC | |
| Apta_406 | DNA | AAAGCCCACTGCATAGAGGCCGCTCAACATGCGAGCCGGT | |
| Apta_876 | DNA | GTTGCGAAAGACAACGAATGCTTTGCCTGCCATAATTTGC | |
| Apta_945 | DNA | GTTAGACGCTGGACCGCGTGTGATCGGGTCAGCTGCAATT | |
| Apta_1216 | DNA | AGCGTCCTCTGCATAGTGATTTACGCTATGTGTACAGTGC | |
| Apta_593 | DNA | CTCTCGGGACGACAGCCGAGTTGATTCAACAGCCGAGTCGTCCC | |
| Apta_753 | DNA | GGGCACAGACGGAAGATGAGAATTGTGGGGCTTAGTATAGTGAGGTGCGTGT | |
| Apta_63 | DNA | GACCCGTGGTAGGGTAGGATGGGGTGGTC |
DEV TOOL
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