AptaDB: Result

Interaction ID: 37

Aptamer sequence: TCTATAAACTCTGTCCGGTTTTATAATAATAACATTTTAC

Target unique ID: O15243

Aptamer ID	Aptamer descriptor	Target chemistry	Target name	Affinity	Binding Conditions/Buffer	PubMed ID
Apta_37	Lep4	Protein	OBRG_HUMAN	1.47 μM	25 mM Tris,192 mM glycine, and 5 mM potassium phosphate (pH 8.3) (1 x TGK) buffer	23232067

Aptamer Sequence:	TCTATAAACTCTGTCCGGTTTTATAATAATAACATTTTAC
The optimal secondary structure in dot-bracket notation:	..((((((.((.....)).))))))...............
The centroid secondary structure in dot-bracket notation:	..((((((((......))).)))))...............

The MFE structure

The Centroid structure

The mountain plot representation

Type	Detail	Type	Detail
Aptamer ID	Apta_37	Description	Lep4
Aptamer chemistry	DNA	Length	40 nt
GC content	25.0%	Molecular weight	12,192.90 Da
Molarity of 1 μg/μl solution	82.01 μM	Number of G-quadruplexes	No QGRS found
G-Score	N/A	Function	Targeted binding
Sequence	TCTATAAACTCTGTCCGGTTTTATAATAATAACATTTTAC
Applications	Binding to target proteins

Type	Detail	Type	Detail
Uniprot ID	O15243	Protein name	Leptin receptor gene-related protein (Endospanin-1) (Leptin receptor overlapping transcript protein) (OB-R gene-related protein) (OB-RGRP)
Gene name(s)	LEPROT LEPR OBR	Organism	Leptin receptor gene-related protein (Endospanin-1) (Leptin receptor overlapping transcript protein) (OB-R gene-related protein) (OB-RGRP)
Status	reviewed in uniprot database	Length	131
Mass	14,254	Activity regulation	N/A
Involvement in disease	N/A	Catalytic activity	N/A
PDB ID(s)	N/A
Function	Negatively regulates leptin receptor (LEPR) cell surface expression, and thus decreases response to leptin.

	Interaction ID	37
	Target type	Protein
	Target unique ID	O15243
	Activity	1.47 μM
	Binding Conditions/Buffer	25 mM Tris,192 mM glycine, and 5 mM potassium phosphate (pH 8.3) (1 x TGK) buffer
	Assay	Determination of binding affinities using fluorescence intensity. A constant concentration of protein was immobilized onto a 96-well plate and blocked with 5% BSA. Each well was incubated with a different concentration of aptamer (0–5000 nM). After several washing steps, the fluorescence intensity of the DNA was measured in each well surface and the corrected signal was normalized using the BSA-only control. Fluorescence intensities (in relative fluorescence units, RFU) were plotted against the aptamer concentration (in lM), and the binding affinity KD was calculated using nonlinear regression on GraphPad Prism 5. Experiments were performed in triplicate
	PubMed ID	23232067

Aptamer ID	Aptamer chemistry	Sequence	Similarity
Apta_970	DNA	TTATGTAGCATTTATGAAATTTTTAAATCAATTTACTATTGGTGGTATCC	54.00%
Apta_972	DNA	TGACAGTACGTACTGAACTAATTTATAAGTAATTTGCTATGGTGGTATCC	54.00%
Apta_510	DNA	TGTAATTTGTCTGCAGCGGTTCTTGATCGCTGACACCATATTATGAAGA	53.06%
Apta_604	DNA	TCCTGACCTTAGCCTATGTTTTCTCTACCGGCTATGTTAC	52.50%
Apta_1198	DNA	CTTTTAAGAACCGGACGAACTAGAATATTATATTTT	52.50%
Apta_309	DNA	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC	51.02%
Apta_309	DNA	TAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTAC	51.02%
Apta_285	DNA	GCCACTCCGTCACTTTAGTGGTTTTTTTTATAAATGCTTC	50.00%
Apta_446	DNA	AGCCATGACGATGTCGTTACGTAGATGCATAGACTCCTAA	50.00%
Apta_480	DNA	CGGCACACCTCGGTCGGTTTGTATGGCGAGCTGGTTTTTT	50.00%
Apta_614	DNA	AATCGCATTACCTTATACCAGCTTATTCAATTACGTCTGCACATACCAGCTTATTCAATT	50.00%
Apta_973	DNA	TATGTAATGTTGACGTAATTTATCTCTGTGAGAATAAGCAGGTGGTATCC	50.00%
Apta_974	DNA	CATGGTGTCAATACTATACCTTTTGCCGATGGTAACAGTTGGTGGTATCC	50.00%
Apta_1155	DNA	TAGTTTTATTTGTTCATTTTGTGTATTGTTTTGA	50.00%
Apta_1177	DNA	TCCTACTCGTGATATCGGTGTTTATGGTGTCTGTCTTCATGTAGGA	50.00%
Apta_1214	DNA	GTCCATTATTCTGGTAGCGTTGAACAACATTCAACACGCC	50.00%
Apta_1278	DNA	CCATGATTCAACTTTACTGGTCTTGTCTTGGCTAGTCGTGTGTCATTCCCTAAGG	49.09%
Apta_1280	DNA	TCATATCCTTCTTGGTATCATTGCGTCATTTAGTCATTCATGTTATTCCCGAGGG	49.09%
Apta_644	DNA	TCAATCTAACCATGCATGCAGTTTAGGCAGGATTCGTTATCGCAA	48.89%
Apta_969	DNA	TAACCACCCCACCTCGCTCCCGTGACACTAATGCTAATTCCAA	48.84%

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