Aptamer-target interaction descriptor
Aptamer-target interaction information
Interaction ID: 1129
Aptamer sequence: CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT
Target unique ID: P01112
Aptamer ID Aptamer descriptor Target chemistry Target name Affinity Binding Conditions/Buffer PubMed ID
Apta_1075 aptamer3 for h-Ras Protein RASH_HUMAN 560 nM 50 mM Tris-HCl 16448086
Structure information of aptamer
Aptamer Sequence: CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT
The optimal secondary structure in dot-bracket notation: ..((((((...(((.((((((...(((((...................)))))...))))))..)))...))))))....
The centroid secondary structure in dot-bracket notation: ..((((((...(((.((((((...(((((...................)))))...))))))..)))...))))))....
The MFE structure
The Centroid structure
The mountain plot representation
Aptamer information
Type Detail Type Detail
Aptamer ID Apta_1075 Description aptamer3 for h-Ras
Aptamer chemistry DNA Length 80 nt
GC content 53.8% Molecular weight 24,492.66 Da
Molarity of 1 μg/μl solution 40.83 μM Number of G-quadruplexes No QGRS found
G-Score N/A Function Targeted therapy
Sequence CTTCTGCCCGCCTCCTTCCTACTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT
Applications Applications in cancer research, development of cancer diagnostics, and treatment of cancer
Target information
Type Detail Type Detail
Uniprot ID P01112 Protein name GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed]
Gene name(s) HRAS HRAS1 Organism GTPase HRas (EC 3.6.5.2) (H-Ras-1) (Ha-Ras) (Transforming protein p21) (c-H-ras) (p21ras) [Cleaved into: GTPase HRas, N-terminally processed]
Status reviewed in uniprot database Length 189
Mass 21,298 Activity regulation Alternates between an inactive form bound to GDP and an active form bound to GTP
Involvement in disease Costello syndrome (CSTLO) Catalytic activity Reaction=GTP + H2O = GDP + H(+) + phosphate
PDB ID(s)
121P;1AA9;1AGP;1BKD;1CLU;1CRP;1CRQ;1CRR;1CTQ;1GNP;1GNQ;1GNR;1HE8;1IAQ;1IOZ;1JAH;1JAI;1K8R;1LF0;1LF5;1LFD;1NVU;1NVV;1NVW;1NVX;1P2S;1P2T;1P2U;1P2V;1PLJ;1PLK;1PLL;1Q21;1QRA;1RVD;1WQ1;1XCM;1XD2;1XJ0;1ZVQ;1ZW6;221P;2C5L;2CE2;2CL0;2CL6;2CL7;2CLC;2CLD;2EVW;2LCF;2LWI;2N42;2N46;2Q21;2QUZ;2RGA;2RGB;2RGC;2RGD;2RGE;2RGG;2UZI;2VH5;2X1V;3DDC;3I3S;3K8Y;3K9L;3K9N;3KKM;3KKN;3KUD;3L8Y;3L8Z;3LBH;3LBI;3LBN;3LO5;3OIU;3OIV;3OIW;3RRY;3RRZ;3RS0;3RS2;3RS3;3RS4;3RS5;3RS7;3RSL;3RSO;3TGP;421P;4DLR;4DLS;4DLT;4DLU;4DLV;4DLW;4DLX;4DLY;4DLZ;4DST;4DSU;4EFL;4EFM;4EFN;4G0N;4G3X;4K81;4L9S;4L9W;4NYI;4NYJ;4NYM;4Q21;4RSG;4URU;4URV;4URW;4URX;4URY;4URZ;4US0;4US1;4US2;4XVQ;4XVR;521P;5B2Z;5B30;5E95;5P21;5VBE;5VBZ;5WDO;5WDP;5WDQ;5WFO;5WFP;5WFQ;5WFR;5WPL;5X9S;5ZC6;621P;6AMB;6AXG;6BVI;6BVJ;6BVK;6BVL;6BVM;6CUO;6CUP;6CUR;6D55;6D56;6D59;6D5E;6D5G;6D5H;6D5J;6D5L;6D5M;6D5V;6D5W;6DZH;6E6C;6E6P;6MQT;6NTC;6NTD;6Q21;6V94;6V9F;6V9J;6V9L;6V9M;6V9N;6V9O;6ZJ0;6ZL3;721P;7DPH;7DPJ;7JHP;7JIF;7JIG;7JIH;7JII;7L0F;7L0G;7OG9;7OGA;7OGB;7OGC;7OGD;7OGE;7OGF;821P;
Function Involved in the activation of Ras protein signal transduction.
Activity data

No relevant experimental diagram

 Interaction ID 1129
Target type Protein
Target unique ID P01112
Activity 560 nM
Binding Conditions/Buffer

50 mM Tris-HCl
Assay

N/A
PubMed ID 16448086
Similar aptamers
Aptamer ID Aptamer chemistry Sequence Similarity
Apta_1073 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACATCCCCATAGGAGACGAGATAGGCGGACACT
98.75%
Apta_1076 DNA CTTCTGCCCGCCTCCTTCCTATTAGGGTGCTTCGCGAAGTTCATTTTACACCCCCATAGGAGACGAGATAGGCGGACACT
97.50%
Apta_1030 DNA CTTCTGCCCGCCTCCTTCCCGGGTTACAGGATCGTAAATCTTATTCGTTGCTCGATCGGGAGACGAGATAGGCGGACACT 71.25%
Apta_1074 DNA CTTCTGCCCGCCTCCTTCCGCCACAGTATTATGTATCACAGTGCTTCATCTAATATGAGGAGACGAGATAGGCGGACACT 71.25%
Apta_1028 DNA CTTCTGCCCGCCTCCTTCCGGCGTCCCTGCGCACTGTATAACGTACACACGATACGTGGGAGACGAGATAGGCGGACACT 70.00%
Apta_1029 DNA CTTCTGCCCGCCTCCTTCCCGGTCGAGACCCGGGTTTACTTCCTCGGTAGTGTTCGAGGGAGACGAGATAGGCGGACACT 67.50%
Apta_737 DNA ATCGTCTGCTCCGTCCAATAGTGCATTGAAACTTCTGCATCCTCGTTTGGTGTGAGGTCGTGC 53.75%
Apta_448 DNA TAACTCACTCCATACTCACTTGCTGATTCGCCAACAACACACCCTTAAACAGTCCC 51.25%
Apta_534 DNA ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT 51.25%
Apta_826 DNA ATCCGTCACACCTGCTCTGCACGGGCTCAGTTTGGCTTTGTATCCTAAGAGGGATGGTGTTGGCTCCCGTAT 51.25%
Apta_884 DNA ATACCAGCTTATTCAATTTGCACCACTTATTGTTACTAATCTGAGATAGTAAGTGCAATCT 51.25%
Apta_897 DNA ATACGGGAGCCAACACCACTCTTATTTCCGGAAGTGGTTCTTCAACAGAGGAATAGAGCAGGTGTGACGGAT 51.25%
Apta_534 DNA ATCCGTCACACCTGCTCTTGTAGGGTTCCCACCCAATTCAGTTCCGTTAAACCATGGTGTTGGCTCCCGTAT 51.25%
Apta_922 DNA ATCCAGAGTGACGCAGCACACCGTTTGTATTCTGCATTGTTTTGCATTCTACATGGACACGGTGGCTTAGT 51.25%
Apta_1051 DNA ATCCGTCACACCTGCTCTATACACATTGTGTTTACTCCCAGTTTTTTAGACTTATGGTGTTGGCTCCCGTAT 51.25%
Apta_1111 DNA GTGCGTCGCATGATCGCGATTAGTGCGACCATGTACGATTCTCACGACAACGACACTGTACTTGAGGACTGTCTCC 51.25%
Apta_435 DNA CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA 51.11%
Apta_741 DNA ATCGTCTGCTCCGTCCAATAGCCTCACCCTGCATAATTTATAGACTACACTTAGGAATCGCTGCATTTGGTGTGAGGTCGTGC 50.60%
Apta_1184 DNA GAATTCAGTCGGACAGCGTAATCCCTTGTGTTTGACGTTCGAAGATTATTTCTATCTTTTAGGATGGACGAATATCGTCTCCC 50.60%
Apta_804 DNA GCGGGATCCTATGACGCATTGACCCACAACACACTACTGTCGCTCGGTTCGAACTTCGTGCGACTGTTCCCTATAGTGAGTCGTATTAGAATTCCGC 50.52%

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AptaDB is an online database we developed to integrate comprehensive information about aptamer.
The detail of this database is described here:

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