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Primary-literature peptidemedium evidence

PEPDIA-PLP-002465

RKW

Aliases: RKW

Normalized sequence

RKWILKWLRTWKK

13 amino acids

Release PepDiana-V1-20260620

56

Activity records

2

Source papers

34

Positive

22

Negative / threshold

Versioned scientific descriptors

Physicochemical properties and standard representations

calculated sequence and structure

Reported properties

No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.

Computed properties

physchem-20260620

Molecular weight

1842.2823 Da

Molecular formula

C91H144N26O15

Theoretical pI

12

Net charge at pH 7

5.7561

GRAVY

-1.2231

Instability index

30.2615

Aromaticity

0.2308

Topological polar surface area

708 Å²

cLogP

-0.0505

Hydrogen-bond donors

28

Hydrogen-bond acceptors

21

Rotatable bonds

62

Method: PepDiana sequence/structure descriptor pipeline

Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6

Calculated: 2026-06-20T11:01:38.358971+00:00

Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200

Sequence representations

representations-20260620

ProForma 2.0

validated simple linear

RKWILKWLRTWKK

HELM 2.0

validated simple linear

PEPTIDE1{R.K.W.I.L.K.W.L.R.T.W.K.K}$$$$V2.0

Canonical SMILES

validated

CCC(C)C(NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C(CCCCN)NC(=O)C(N)CCCNC(=N)N)C(=O)NC(CC(C)C)C(=O)NC(CCCCN)C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)NC(CC(C)C)C(=O)NC(CCCNC(=N)N)C(=O)NC(C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)O)C(C)O

Isomeric SMILES

validated

CC[C@H](C)[C@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)[C@@H](C)O

Paper-level observations

Experimental evidence grouped by activity

Antimicrobial 52 records

PMC12292521

52 observations

AntimicrobialAntibacterialPositive observation

Table 2: Escherichia coli MBC 10 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Salmonella Typhimurium MBC 10 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Salmonella Typhimurium
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Listeria monocytogenes MBC 10 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Listeria monocytogenes
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Staphylococcus aureus MBC 15 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 15.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Pseudomonas aeruginosa MBC 5 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 5.0 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Campylobacter jejuni MBC 80 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 80.0 µM
Target: Campylobacter jejuni
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Monophasic Salmonella Typhimurium MBC 20 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 20.0 µM
Target: Monophasic Salmonella Typhimurium
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 2: Salmonella Napoli MBC 20 µM at 1.0 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 20.0 µM
Target: Salmonella Napoli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: Escherichia coli MBC 10.0 µM at 1.0 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Escherichia coli
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: Salmonella Typhimurium MBC 10.0 µM at 1.0 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Salmonella Typhimurium
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: Listeria monocytogenes MBC 10.0 µM at 1.0 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 10.0 µM
Target: Listeria monocytogenes
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: Staphylococcus aureus MBC 20.0 µM at 1.0 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 20.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 3: Pseudomonas aeruginosa MBC 5.0 µM at 1.0 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 5.0 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Acinetobacter baumannii (CRAB) MIC 50 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 50.0 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Acinetobacter baumannii (CRAB) MBC 50 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 50.0 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Acinetobacter baumannii (CRAB) MIC 75 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 75.0 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 5: Acinetobacter baumannii (CRAB) MBC >75 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: >75 µM
Target: Acinetobacter baumannii
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Pseudomonas aeruginosa MIC 20 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 20.0 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Pseudomonas aeruginosa MBC 20 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 20.0 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Pseudomonas aeruginosa MIC 75 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 75.0 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 5: Pseudomonas aeruginosa MBC >75 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: >75 µM
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Staphylococcus aureus (MRSA) MIC 50 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 50.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Staphylococcus aureus (MRSA) MBC 50 µM at 5 × 10^3 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 50.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Staphylococcus aureus (MRSA) MIC 50 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MIC
Value: 50.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 5: Staphylococcus aureus (MRSA) MBC 50 µM at 5 × 10^5 CFU/mL.

Mention PMC12292521::PEP000001PMC12292521table

Measure: MBC
Value: 50.0 µM
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-BiofilmPositive observation

Figure 6 caption: RKW significantly inhibited biofilm production by S. aureus MRSA (ANOVA, p<0.01) at ½ and ¼ MIC.

Mention PMC12292521::PEP000001PMC12292521figure

Measure: biofilm_inhibition
Value: significant inhibition (p<0.01)
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAnti-BiofilmPositive observation

Figure 6 caption: RKW significantly inhibited biofilm production by P. aeruginosa (ANOVA, p<0.05) at ½ and ¼ MIC.

Mention PMC12292521::PEP000001PMC12292521figure

Measure: biofilm_inhibition
Value: significant inhibition (p<0.05)
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. aureus ATCC 6538 at 100 µM, log R = 3.13, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 3.13 (Not active) log10
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. aureus ATCC 6538 at 50 µM, log R = 3.13, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 3.13 (Not active) log10
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. aureus ATCC 6538 at 20 µM, log R = 3.13, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 3.13 (Not active) log10
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. aureus ATCC 6538 at 10 µM, log R = 2.59, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 2.59 (Not active) log10
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. aureus ATCC 6538 at 5 µM, log R = 2.25, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 2.25 (Not active) log10
Target: Staphylococcus aureus
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: P. aeruginosa ATCC 15442 at 100 µM, log R > 5.25, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R > 5.25 (Active) log10
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: P. aeruginosa ATCC 15442 at 50 µM, log R > 5.25, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R > 5.25 (Active) log10
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: P. aeruginosa ATCC 15442 at 20 µM, log R > 5.25, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R > 5.25 (Active) log10
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: P. aeruginosa ATCC 15442 at 10 µM, log R > 5.25, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R > 5.25 (Active) log10
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: P. aeruginosa ATCC 15442 at 5 µM, log R > 5.25, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R > 5.25 (Active) log10
Target: Pseudomonas aeruginosa
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 6: S. epidermidis ATCC 12228 at 100 µM, log R = 5.08, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 5.08 (Active) log10
Target: Staphylococcus epidermidis
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 6: S. epidermidis ATCC 12228 at 50 µM, log R = 5.08, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 5.08 (Active) log10
Target: Staphylococcus epidermidis
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialPositive observation

Table 6: S. epidermidis ATCC 12228 at 20 µM, log R = 5.08, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 5.08 (Active) log10
Target: Staphylococcus epidermidis
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. epidermidis ATCC 12228 at 10 µM, log R = 4.90, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 4.90 (Not active) log10
Target: Staphylococcus epidermidis
Assay: Not reported by source

Source publication

AntimicrobialAntibacterialNegative / threshold

Table 6: S. epidermidis ATCC 12228 at 5 µM, log R = 4.71, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 4.71 (Not active) log10
Target: Staphylococcus epidermidis
Assay: Not reported by source

Source publication

AntimicrobialAntifungalPositive observation

Table 6: C. albicans ATCC 10231 at 100 µM, log R = 4.16, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 4.16 (Active) log10
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAntifungalPositive observation

Table 6: C. albicans ATCC 10231 at 50 µM, log R = 4.16, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 4.16 (Active) log10
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAntifungalPositive observation

Table 6: C. albicans ATCC 10231 at 20 µM, log R = 4.16, Active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 4.16 (Active) log10
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: C. albicans ATCC 10231 at 10 µM, log R = 3.57, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 3.57 (Not active) log10
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: C. albicans ATCC 10231 at 5 µM, log R = 1.71, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = 1.71 (Not active) log10
Target: Candida albicans
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: A. brasiliensis ATCC 16404 at 100 µM, log R = -0.09, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = -0.09 (Not active) log10
Target: Aspergillus brasiliensis
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: A. brasiliensis ATCC 16404 at 50 µM, log R = -0.09, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = -0.09 (Not active) log10
Target: Aspergillus brasiliensis
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: A. brasiliensis ATCC 16404 at 20 µM, log R = -0.09, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = -0.09 (Not active) log10
Target: Aspergillus brasiliensis
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: A. brasiliensis ATCC 16404 at 10 µM, log R = -0.29, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = -0.29 (Not active) log10
Target: Aspergillus brasiliensis
Assay: Not reported by source

Source publication

AntimicrobialAntifungalNegative / threshold

Table 6: A. brasiliensis ATCC 16404 at 5 µM, log R = -0.28, Not active.

Mention PMC12292521::PEP000001PMC12292521table

Measure: other
Value: log R = -0.28 (Not active) log10
Target: Aspergillus brasiliensis
Assay: Not reported by source

Source publication

Toxicity 4 records

PMC12947016

3 observations

ToxicityGeneral ToxicPositive observation

After 48 h of treatment, the peptide's impact on worm survival was evaluated. As shown in Figure 8B,C, RKW did not exhibit any toxicity at the tested concentrations.

Mention PMC12947016::PEP000001PMC12947016text

Measure: survival_rate
Value: RKW did not exhibit any toxicity at the tested concentrations
Target: Caenorhabditis elegans
Assay: Not reported by source

Source publication

ToxicityGeneral ToxicPositive observation

As shown in Figure 9, treatment with peptide at both 10 and 50 μM concentrations did not significantly affect egg-laying (Figure 9A–C) or embryonic survival (Figure 9B,C) compared to controls.

Mention PMC12947016::PEP000001PMC12947016text

Measure: survival_rate
Value: treatment with peptide at both 10 and 50 μM concentrations did not significantly affect egg-laying or embryonic survival
Target: Caenorhabditis elegans
Assay: Not reported by source

Source publication

ToxicityGeneral ToxicNegative / threshold

The results revealed no significant increase in DNA damage-induced apoptosis following peptide exposure (Figure 10).

Mention PMC12947016::PEP000001PMC12947016text

Measure: other
Value: no significant increase in DNA damage-induced apoptosis
Target: Caenorhabditis elegans
Assay: Not reported by source

Source publication

PMC12292521

1 observations

ToxicityCytotoxicPositive observation

Figure 7 caption and Section 3.6: RKW at 5, 10, 25 µM did not result in any significant effect on viability of BALB 3T3 cells.

Mention PMC12292521::PEP000001PMC12292521figure

Measure: cell_viability
Value: no significant effect on viability
Target: BALB 3T3 clone A31
Assay: Not reported by source

Source publication

Citation and release provenance

Cite this identity as PepDiana record PEPDIA-PLP-002465, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.

Record updated: 2026-06-22T07:14:26.229247+00:00