ProForma 2.0
validated simple linear
GLLHLLHHLLHHNH
Back to Primary literature
Primary-literature peptidehigh evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-001008
LH12
Aliases: LH12
Normalized sequence
GLLHLLHHLLHHNH
14 amino acids
Release PepDiana-V1-20260620
43
Activity records
1
Source papers
36
Positive
7
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
1690.9506 Da
Molecular formula
C78H119N27O16
Theoretical pI
7.2059
Net charge at pH 7
0.2831
GRAVY
-0.0214
Instability index
26.2
Aromaticity
0
Topological polar surface area
656.79 Ų
cLogP
-2.7211
Hydrogen-bond donors
22
Hydrogen-bond acceptors
22
Rotatable bonds
53
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{G.L.L.H.L.L.H.H.L.L.H.H.N.H}$$$$V2.0
Canonical SMILES
validated
CC(C)CC(NC(=O)CN)C(=O)NC(CC(C)C)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(Cc1c[nH]cn1)C(=O)NC(CC(N)=O)C(=O)NC(Cc1c[nH]cn1)C(=O)O
Isomeric SMILES
validated
CC(C)C[C@H](NC(=O)CN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O
Paper-level observations
Experimental evidence grouped by activity
Antimicrobial 40 records
PMC9788686
40 observationsAntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against S. mutans at pH 5.5 with MICs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 12.00 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against S. mutans at pH 5.5 with MBCs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 12.00 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MICs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 21.33 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MBCs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 21.33 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against E. coli at pH 5.5 with MICs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 12.00 μg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against E. coli at pH 5.5 with MBCs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 12.00 μg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MICs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 21.33 μg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MBCs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 21.33 μg/mL
- Target
- Escherichia coli
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against S. aureus at pH 5.5 with MICs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 12.00 μg/mL
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail LH12 displayed stronger antibacterial activity against S. aureus at pH 5.5 with MBCs ranging from 12.00 μg/mL to 64.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 12.00 μg/mL
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MICs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MIC
- Value
- 21.33 μg/mL
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail MBCs at pH 7.2 ranging from 21.33 μg/mL to more than 128.00 μg/mL. Figure 2d.
- Measure
- MBC
- Value
- 21.33 μg/mL
- Target
- Staphylococcus aureus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MICs of LH12 against S. mutans were from 8.00 μg/mL to 16.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 8.00 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBCs were from 16.00 μg/mL to 32.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 16.00 μg/mL
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MICs of LH12 against A. viscosus were from 8.00 μg/mL to 16.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 8.00 μg/mL
- Target
- Actinomyces viscosus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBCs were from 16.00 μg/mL to 32.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 16.00 μg/mL
- Target
- Actinomyces viscosus
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MICs of LH12 against A. naeslundii were from 8.00 μg/mL to 16.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 8.00 μg/mL
- Target
- Actinomyces naeslundii
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBCs were from 16.00 μg/mL to 32.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 16.00 μg/mL
- Target
- Actinomyces naeslundii
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MICs of LH12 against L. casei were from 8.00 μg/mL to 16.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 8.00 μg/mL
- Target
- Lactobacillus casei
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBCs were from 16.00 μg/mL to 32.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 16.00 μg/mL
- Target
- Lactobacillus casei
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MICs of LH12 against L. fermentum were from 8.00 μg/mL to 16.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 8.00 μg/mL
- Target
- Lactobacillus fermentum
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBCs were from 16.00 μg/mL to 32.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 16.00 μg/mL
- Target
- Lactobacillus fermentum
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MIC of LH12 against S. salivarius was 48.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- 48.00 μg/mL
- Target
- Streptococcus salivarius
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail The MBC was 64.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- 64.00 μg/mL
- Target
- Streptococcus salivarius
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. sanguinis were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus sanguinis
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. sanguinis were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus sanguinis
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. gordonii were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus gordonii
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. gordonii were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus gordonii
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. mitis were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MIC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus mitis
- Assay
- Not reported by source
AntimicrobialAntibacterialNegative / threshold
Record detail The MICs and MBCs of LH12 against S. mitis were no less than 128.00 μg/mL. Figure 4a.
- Measure
- MBC
- Value
- ≥128.00 μg/mL
- Target
- Streptococcus mitis
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail 8 μg/mL LH12 could reduce 84.04% of biofilm mass. Figure 6a.
- Measure
- biofilm_inhibition
- Value
- 84.04%
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail 16 μg/mL and 32 μg/mL LH12 totally inhibited the biofilm formation. Figure 6a.
- Measure
- biofilm_inhibition
- Value
- totally inhibited
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAntibacterialPositive observation
Record detail 32 μg/mL LH12 reduced the viable S. mutans in the preformed biofilms. Figure 6e.
- Measure
- other
- Value
- reduced
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail 32 μg/mL LH12 reduced the biofilm thickness to a half of that in the control group. Figure 6f.
- Measure
- biofilm_inhibition
- Value
- reduced the biofilm thickness to a half
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialOtherPositive observation
Record detail 16 μg/mL and 32 μg/mL LH12 could decrease the lactic acid production of the preformed biofilms. Figure 6c.
- Measure
- percent_inhibition
- Value
- decreased
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail 16 μg/mL and 32 μg/mL LH12 could decrease the water-insoluble EPS synthesis of the preformed biofilms. Figure 6d.
- Measure
- percent_inhibition
- Value
- decreased
- Target
- Streptococcus mutans
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail LH12 could inhibit the formation of the dual-species biofilm. Figure 7a.
- Measure
- biofilm_inhibition
- Value
- inhibited
- Target
- Streptococcus mutans; Streptococcus gordonii
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail LH12 could reduce its strength to make it easier to remove. Figure 7b.
- Measure
- biofilm_inhibition
- Value
- reduced
- Target
- Streptococcus mutans; Streptococcus gordonii
- Assay
- Not reported by source
AntimicrobialOtherPositive observation
Record detail The short-term treatment of LH12 reduced the lactic acid production of the dual-species biofilm. Figure 7d.
- Measure
- percent_inhibition
- Value
- reduced
- Target
- Streptococcus mutans; Streptococcus gordonii
- Assay
- Not reported by source
AntimicrobialAnti-BiofilmPositive observation
Record detail The short-term treatment of LH12 reduced the water-insoluble EPS synthesis of the dual-species biofilm. Figure 7e.
- Measure
- percent_inhibition
- Value
- reduced
- Target
- Streptococcus mutans; Streptococcus gordonii
- Assay
- Not reported by source
Toxicity 3 records
PMC9788686
3 observationsToxicityCytotoxicPositive observation
Record detail Only at a high concentration of 128 μg/mL would LH12 show slight cytotoxicity on HGECs with an inhibition ratio around 9.64% but without a significant reduction of the OD450 value after 45 min treatment. Figure 3a.
- Measure
- percent_inhibition
- Value
- 9.64%
- Target
- HGECs
- Assay
- Not reported by source
ToxicityHemolyticPositive observation
Record detail LH12 showed no hemolytic toxicity below 64 μg/mL and exhibited an increase of hemolysis ratio to 17.15% at 128 μg/mL. Figure 3c.
- Measure
- hemolysis
- Value
- 17.15%
- Target
- Not reported by source
- Assay
- Not reported by source
ToxicityHemolyticNegative / threshold
Record detail LH12 showed no hemolytic toxicity below 64 μg/mL. Figure 3c.
- Measure
- hemolysis
- Value
- no hemolytic toxicity
- Target
- Not reported by source
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-001008, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00