ProForma 2.0
validated simple linear
CYGSR
Back to Primary literature
Primary-literature peptidehigh evidence PepDiana evidence grade for a literature-derived identity; Medium indicates retained review flags, while High has no unresolved review flag.
PEPDIA-PLP-000379
C-CR5
Aliases: C-CR5, L-CR5
Normalized sequence
CYGSR
5 amino acids
Release PepDiana-V1-20260620
21
Activity records
1
Source papers
18
Positive
3
Negative / threshold
Versioned scientific descriptors
Physicochemical properties and standard representations
Reported properties
No separate physicochemical property was reported in the primary-literature identity record. PepDiana release values are shown as computed properties.
Computed properties
physchem-20260620Molecular weight
584.6457 Da
Molecular formula
C23H36N8O8S
Theoretical pI
8.222
Net charge at pH 7
0.7492
GRAVY
-0.9
Instability index
29.54
Aromaticity
0.2
Topological polar surface area
282.08 Ų
cLogP
-3.8974
Hydrogen-bond donors
12
Hydrogen-bond acceptors
10
Rotatable bonds
17
Method: PepDiana sequence/structure descriptor pipeline
Tool: Biopython+RDKit · Biopython 1.85; RDKit 2024.09.6
Calculated: 2026-06-20T11:01:38.358971+00:00
Assumptions: ph=7; termini=free N-terminus and free C-terminus for simple linear peptides; stereochemistry=standard L-amino acids; smiles_length_limit=200
Sequence representations
HELM 2.0
validated simple linear
PEPTIDE1{C.Y.G.S.R}$$$$V2.0
Canonical SMILES
validated
N=C(N)NCCCC(NC(=O)C(CO)NC(=O)CNC(=O)C(Cc1ccc(O)cc1)NC(=O)C(N)CS)C(=O)O
Isomeric SMILES
validated
N=C(N)NCCC[C@H](NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CS)C(=O)O
Paper-level observations
Experimental evidence grouped by activity
Antioxidant 19 records
PMC12839123
19 observationsAntioxidantPositive observation
Record detail The IC50 of L-CR5 was calculated as 377.83 ± 34.32 μg/mL (646.25 ± 58.70 μM). Figure 3 caption: IC50 = 377.83 ± 34.32 μg/mL; (646.25 ± 58.70 μM), n = 3.
- Measure
- other
- Value
- 377.83 ± 34.32 μg/mL (646.25 ± 58.70 μM)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantPositive observation
Record detail the IC50 of C-CR5 was found to be 16.92 ± 0.47 μg/mL (29.86 ± 0.83 μM). Figure 3 caption: IC50 = 16.92 ± 0.47 μg/mL; (29.86 ± 0.83 μM), n = 3.
- Measure
- other
- Value
- 16.92 ± 0.47 μg/mL (29.86 ± 0.83 μM)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantAntibacterialPositive observation
Record detail The scavenging ability of L-CR5 gradually increased from 18% to 53% in the range of 25–400 μg/mL.
- Measure
- percent_inhibition
- Value
- 18% (at 25 μg/mL)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantAntibacterialPositive observation
Record detail The scavenging ability of L-CR5 gradually increased from 18% to 53% in the range of 25–400 μg/mL.
- Measure
- percent_inhibition
- Value
- 53% (at 400 μg/mL)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantAntibacterialPositive observation
Record detail C-CR5 showed very high activity from 73% to 83% under the same concentration condition [25–400 μg/mL].
- Measure
- percent_inhibition
- Value
- 73% (at 25 μg/mL)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantAntibacterialPositive observation
Record detail C-CR5 showed very high activity from 73% to 83% under the same concentration condition [25–400 μg/mL].
- Measure
- percent_inhibition
- Value
- 83% (at 400 μg/mL)
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantPositive observation
Record detail IC50 of L-CR5 was calculated as 377.83 ± 34.32 μg/mL (646.25 ± 58.70 μM).
- Measure
- other
- Value
- 646.25 ± 58.70 μM
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantPositive observation
Record detail IC50 of C-CR5 was found to be 16.92 ± 0.47 μg/mL (29.86 ± 0.83 μM).
- Measure
- other
- Value
- 29.86 ± 0.83 μM
- Target
- Not reported by source
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail The IC50 of L-CR5 was calculated as 680.63 ± 15.68 μg/mL (1164.0 ± 27.3 μM). Figure 4 caption: IC50 = 680.63 ± 15.68 μg/mL; (1164.0 ± 27.3 μM), n = 3.
- Measure
- other
- Value
- 680.63 ± 15.68 μg/mL (1164.0 ± 27.3 μM)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail the IC50 of C-CR5 was identified as 104.2 ± 5.3 μg/mL (184.0 ± 9.4 μM). Figure 4 caption: IC50 = 104.2 ± 5.3 μg/mL; (184.0 ± 9.4 μM), n = 3.
- Measure
- other
- Value
- 104.2 ± 5.3 μg/mL (184.0 ± 9.4 μM)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail IC50 of L-CR5 was calculated as 680.63 ± 15.68 μg/mL (1164.0 ± 27.3 μM).
- Measure
- other
- Value
- 1164.0 ± 27.3 μM
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail IC50 of C-CR5 was identified as 104.2 ± 5.3 μg/mL (184.0 ± 9.4 μM).
- Measure
- other
- Value
- 184.0 ± 9.4 μM
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail In the range of 50–800 μg/mL, the tyrosinase inhibitory rate of L-CR5 gradually increased from 1.16% to 57.35%.
- Measure
- percent_inhibition
- Value
- 1.16% (at 50 μg/mL)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail In the range of 50–800 μg/mL, the tyrosinase inhibitory rate of L-CR5 gradually increased from 1.16% to 57.35%.
- Measure
- percent_inhibition
- Value
- 57.35% (at 800 μg/mL)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail C-CR5 showed a rapid increase from 30.91% to 97.79% in the same concentration section [50–800 μg/mL].
- Measure
- percent_inhibition
- Value
- 30.91% (at 50 μg/mL)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantTyrosinase InhibitorPositive observation
Record detail C-CR5 showed a rapid increase from 30.91% to 97.79% in the same concentration section [50–800 μg/mL].
- Measure
- percent_inhibition
- Value
- 97.79% (at 800 μg/mL)
- Target
- mushroom tyrosinase
- Assay
- Not reported by source
AntioxidantMMP-1 InhibitorNegative / threshold
Record detail L-CR5 showed expression levels of 99–100% in the 25–100 μg/mL range, had little tendency to decrease, and no significant inhibitory effect was observed at 100.80% even at 200 and 400 μg/mL.
- Measure
- percent_inhibition
- Value
- 99–100% expression (no inhibition) at 25–100 μg/mL; 100.80% expression at 200–400 μg/mL
- Target
- CCD-986sk
- Assay
- Not reported by source
AntioxidantMMP-1 InhibitorPositive observation
Record detail the expression level of C-CR5 decreased sharply from 200 μg/mL to 63.31%.
- Measure
- percent_inhibition
- Value
- 63.31% expression (36.69% inhibition) at 200 μg/mL
- Target
- CCD-986sk
- Assay
- Not reported by source
AntioxidantMMP-1 InhibitorPositive observation
Record detail decreased to 7.78% at 400 μg/mL, showing almost complete inhibitory effects.
- Measure
- percent_inhibition
- Value
- 7.78% expression (92.22% inhibition) at 400 μg/mL
- Target
- CCD-986sk
- Assay
- Not reported by source
Toxicity 2 records
PMC12839123
2 observationsToxicityCytotoxicNegative / threshold
Record detail As a result of treating the RAW 264.7 macrophages with L-CR5 and C-CR5 at a concentration of 6.25–400 μg/mL, cell viability was maintained at 95% or more in all treatment groups.
- Measure
- cell_viability
- Value
- ≥95%
- Target
- RAW 264.7
- Assay
- Not reported by source
ToxicityCytotoxicNegative / threshold
Record detail As a result of treating the RAW 264.7 macrophages with L-CR5 and C-CR5 at a concentration of 6.25–400 μg/mL, cell viability was maintained at 95% or more in all treatment groups.
- Measure
- cell_viability
- Value
- ≥95%
- Target
- RAW 264.7
- Assay
- Not reported by source
Citation and release provenance
Cite this identity as PepDiana record PEPDIA-PLP-000379, release PepDiana-V1-20260620. Experimental observations retain their paper, mention, and activity-record identifiers.
Record updated: 2026-06-22T07:14:26.229247+00:00